Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
C. elegans lecture #2 Suppressor/enhancer genetics as a discovery system epistasis analyses Cell divisions in the development of the vulva lin-4 mutants were found to be vulvaless Suppressor screening on lin-4(e912) Po EMG mutagenize lin-4(e912) hermaphrodites (no vulva) F1 lin-4(e912)/lin-4(e912); */+ (rare dominant suppressor) F2 lin-4(e912)/lin-4(e912); */* (rare recessive suppressor) What the screen identified: lin-14(lf) and lin-28(lf) cause precocious expression of adult lineage! This is a much more subtle phenotype than lin-4(lf) bag-o-worm phenotype, so not easy to identify in a wild type background but they do emerge in a suppressor screen. Pathway building: epistasis analyses Temporal expression patterns of heterochronic pathway components Mechanisms of actions of microRNAs RNAi