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Biology Principles of Heredity Part I Learning Objectives 1. Define genetics and explain why it is important. 2. Explain what factors govern genetics. 3. Explain how organisms reproduce. 4. Explain what Gregor Mendel learned about genetics. 5. Explain the outcome of a monohybrid cross for complete dominance. Terms Allele Cell Chromosome Complete dominance Cross-pollination Dominant Gametes Gene Genetics Genotype Gregor Mendel Heredity Heterozygous Homozygous Inheritance Meiosis Mitosis Monohybrid cross Terms Phenotype Pollination Principle of Independent Assortment Principle of Segregation Probability Punnett square Recessive Self-pollination Species Zygote I. What is genetics and why is it important to understand genetics? A. Genetics is the study of how traits are passed from parents to offspring 1.The passing of traits from one generation to another is heredity 2. traits are governed by genetics as well as the environment 3. The amount of environmental influence can vary greatly microsoft.com microsoft.com B. Each living thing produces offspring like itself C. Traits 1. common characteristics that distinguish one organism from other kinds of organisms 2. i.e. cats, dogs, humans, and corn plants have different defining traits http://www.acc.umu.se/~zqad/cats/index.html; http://www.fotosearch.com/NGF002/71952708/; http://puppydogweb.com/gallery/labradorretrievers/labradorretriever_tink. D. Organisms within a species have traits that vary and distinguish them as different from each other 1. white leaves vs. green leaves 2. purple seeds vs. white seeds E. Understanding genetics is important because: 1. Gain disease resistance 2. Gain insect resistance 3. Improve environmental adaptation 4. Improve productivity 5. Make a species more suited to cultural practices 6. Obtain a more desirable product II. What governs genetics and how are genes passed from offspring to parents? A. cell - the basic unit of life 1. made up of the cell membrane, cytoplasm, and nucleus 2. cell membrane protects the cell and regulates what can go in and out of the cell 3. cytoplasm contains the organelles which are like small organs that perform a variety of functions that are vital to the cell 4. organelles include the mitochondria, microtubules, ribosomes, endoplasmic reticulum, golgi complex, vacuoles, plastids 5. nucleus is the “brain” of the cell and contains the genetic information that directs the activities of the cell B. Chromosomes 1. found in the nucleus 2. carry the genes which govern specific traits 3. Chromosomes are found in pairs in all cells except in the reproductive cells 4. corn has 20 chromosomes or 10 pairs C. Gene 1. a sequence of DNA that codes for a protein and thus determines a trait 2. Both members of the chromosome pair contain the same genes in the exact same location on the chromosome 3. for any one trait there exists a pair of genes responsible for its expression D. chromosomes numbers corn—20 barley—14 alfalfa—32 potato—48 cotton—52 carrot—18 garden pea—20 lettuce—18 wheat—42 Need animals, etc. Comparative Scale of a Gene Map Mapping of Earth’s Mapping of Cells, Features Chromosomes, and Genes Earth Country State Cell Chromosome Chromosome fragment City Gene People Nucleotide base pairs III. How do organisms reproduce? Cells can reproduce by two main methods in multicellular organisms—mitosis & meiosis A. Mitosis - type of asexual reproduction where two new cells are created from the original cell 1. Each new cell is genetically identical to the parent 2. continues throughout an organism’s life 3. steps of cell cycle Interphase Mitosis prophase metaphase anaphase telophase Cytokinesis a. Interphase - the cell grows in size and replication of the chromosomes occurs b. Mitosis – division of the nucleus c. Cytokinesis - division of the cytoplasm 4. Mitosis is important to the growth of many organisms 5. For example, human cells contain 46 chromosomes a. During the cell cycle, the number of chromosomes is doubled b. the cell divides into two new cells each containing 46 identical chromosomes B. Meiosis - cell division that creates four sex cells 1. occurs in the flower (in angiosperms) to form the sex cells - pollen grains and the embryo sac (contains the egg) 2. Animals - sperm microsoft.com 3. The four new cells or daughter cells are not genetically identical nor are they identical to the parent cell 4. The chromosomes in the daughter cells do not contain chromosomes in pairs (haploid) 5. Steps of Meiosis: Interphase, Prophase I, Metaphase I, Anaphase I, Telophase I, Interphase, Prophase II, Metaphase II, Anaphase II, Telophase II, and Cytokinesis 6. Meiosis allows for the random assortment of parental genes a. Crossing over 1) homologous chromosomes exchange portions of their chromatids 2) results in new combinations of alleles 3) occurs during meiosis I 7. For example, human cells - 46 chromosomes a. The chromosomes replicate — divide once forming two cells containing 46 chromosomes—and then divide again forming four daughter cells of 23 chromosomes b. The egg and sperm cells are examples of cells created through meiosis C. When the gametes which are created by meiosis unite or fertilize sexual reproduction has occurred 1. Offspring produced by sexual reproduction receive half of their genetic information from their female parent and the other half from their male parent microsoft.com D. zygote 1. protoplast resulting from the fusion of gametes 2. the beginning of a new individual in sexual reproduction microsoft.com E. species 1. a group of related organisms that produce fertile offspring Bulldog - http://puppydogweb.com/gallery/bulldogs/bulldog_shallow.jpg; cocker spaniel http://puppydogweb.com/gallery/cockerspaniels/cockerspaniel_wade1.jpg; yorkshire terrier http://puppydogweb.com/gallery/yorkshireterriers/yorkshireterrier_diaz.jpg F. breed, cultivar or variety 1. an international term denoting certain cultivated plants or other organisms that are clearly distinguishable from others by any characteristic 2. when reproduced they retain their distinguishing characters 2. different cultivars can be crossed to obtain organisms with unique characteristics IV. What did Mendel learn about how traits are passed form parents to offspring? A. Gregor Mendel an Austrian monk that conducted experiments on pea plants http://www.accessexcellence.org/RC /AB/BC/Gregor_Mendel.html Figure 11-3 Mendel’s Seven F1 Crosses on Pea Plants Section 11-1 Seed Coat Color Pod Shape Pod Color Smooth Green Seed Shape Seed Color Round Yellow Gray Wrinkled Green White Constricted Round Yellow Gray Smooth Flower Position Plant Height Axial Tall Yellow Terminal Short Green Axial Tall 1. Inheritance a. the acquisition of traits by offspring 2. Heredity a. the passing of traits from parents to offspring 3. The passing of pure traits always results in offspring with the same trait B. Plants pass their traits to their offspring through the process of pollination 1. Pollination - the transfer of pollen from one flower to another flower of the same species 2. Self-pollination - the transfer of pollen from the anther (male) of one flower to the stigma (female) of another flower on the same plant 3. Cross-pollination - the transfer of pollen from an anther on one flower to the stigma on another plant C. Mendel’s Conclusions 1. Principle of Independent Assortment a. there are two factors which govern a particular trait and they are distributed independently microsoft.com b. dominant factor is one that hides the other factor for a particular characteristic 1) Green pea pods and purple flowers 2) In humans, tongue rolling and free ear lobes 3) Represented by capital letters c. recessive factor - one that is hidden by the dominant factor 1) Yellow pea pods and white flowers 2) In humans, dwarfism, straight hairline, short eyelashes, and straight thumbs 3) Represented by lowercase letters 2. Principle of Segregation a. each pair of factors is separated during the formation of the gametes (egg and sperm) b. happens through the process of meiosis D. allele 1. a contrasting form of a gene 2. green pea pods vs. yellow pea pods (G = green, g = yellow) 3. short vs. tall pea plants (T = tall, t = short) E. Generations 1. P generation = parental 2. F1 generation = offspring of P cross 3. F2 generation = offspring of self- pollinated F1 generation V. How is the outcome of a monohybrid cross for complete dominance determined? A. monohybrid cross - a cross between two individuals involving one pair of alleles or traits B. Complete dominance 1. a condition where one allele completely masks or hides the other allele 2. it is completely dominant over the other C. punnett square used to determine the genotype, phenotype, and probability of a genetic cross D. Phenotype - the physical makeup or outward appearance of an organism 1. For example, green pea pods or yellow pea pods E. Genotype - the genetic makeup of an organism - alleles are represented by letters 1. “G” may represent green pea pods while “g” represents yellow pea pods 2. Because green pea pods are dominant to yellow pea pods, the green allele is dominant and the yellow allele is recessive 3. a pea plant with the genotype “GG” or “Gg” is green while one with the genotype “gg” is yellow F. Homozygous 1. the same alleles are present 2. “GG” and “gg” G. Heterozygous 1. different alleles are present 2. “Gg” H. Probability - the chance that a specific event will occur 1. calculated by dividing the number of one kind of event by the total number of events 2. four pea pods and one is yellow probability is ¼ or 25% Summary/Review Plants are an important part of our life. A cell is the basic unit of life. The cell is made up of the cell membrane, cytoplasm, and the nucleus. Cells can reproduce by two main methods in multicellular organisms—mitosis and meiosis. One of the keys to understanding genetics is to understand how to determine the outcome of various genetic crosses. The End I. What is genetics and why is it important to understand genetics? Plants are an important part of our life. They not only supply us with food for nutrition, but they also provide us with medicine, latex, oils, latex, and resins. Because plants are so valuable,humans have tried to manipulate plants for our benefit for thousands of years. microsoft.com What is genetics and why is it important to understand genetics? Early man realized that some plants tasted better than others and that seeds could be harvested and planted to increase the availability of certain plants. Modern methods of plant breeding have dramatically improved crop yield and nutritional quality in modern cultivars, (i.e. the development of hybrid corn in the 1930’s is one of the most important developments in agriculture in the 20th Century). What is genetics and why is it important to understand genetics? The planting of hybrid cultivars and good farming practices have resulted in corn yields of 400 bushels per acre. microsoft.com What is genetics and why is it important to understand genetics? The founding of the International Rice Research Institute (IRRI) in 1962 is another example of how man has manipulated plant genetic information for our benefit. This was one of the cornerstones of the green revolution of the 1960’s. The IRRI developed cultivars which dramatically increased yields. By 1974, modern dwarf rice accounted for more than 99% of irrigated rice averaging 45–80 bushels per acre. What is genetics and why is it important to understand genetics? During the 19th century we learned that genetics governs the traits that are expressed in plants. Today scientists are experimenting with genetic engineering to change the genetic make-up of plants.