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Comparative Analysis of Novel Proteins from the CATH Family of Zinc Peptidases Debanu Das1,2, Abhinav Kumar1,2, Lukasz Jaroszewski1,3 and Ashley Deacon1,2 1Joint Center for Structural Genomics, 2Stanford Synchrotron Radiation Laboratory, Menlo Park, CA 94025, 3Burnham Institute, La Jolla, CA, 92037 I. Introduction In March 2007, the JCSG initiated a study of the CATH homologous superfamily of zinc peptidases (3.40.630.10), which belong to the phosphorylase/hydrolase-like fold in SCOP. These proteins show significant sequence divergence and have a broad phylogenetic distribution across both prokaryotes and eukaryotes. At the time, despite 40 unique experimentally determined structures in the PDB, only half of the family members had reliable homology models. JCSG is improving the structural coverage by determining novel structures which share <30% sequence identity with those in the PDB. Hidden Markov Models from the CATH database were used to identify sequences in the JCSG genome pool. PSI-Blast seeded with these sequences was used to find additional proteins. These two sets yielded 226 unique targets. After removing targets with more than 30% sequence identity to any PDB structure or to any crystallized target from a structural genomics center, 161 targets remained. Further clustering at 90% (in order to avoid nearly identical sequences), produced a set of 137 targets. To date, JCSG has contributed 6 new structures to the family and 7 other targets have been crystallized. We present our progress towards complete structural coverage of this family, highlighting common and variant structural features that support different molecular and cellular roles, focusing on active site residues, ligand binding, protein size and oligomerization state. This analysis may provide insights into structural themes that dictate protein function and also allows modeling of protein structures related by sequence. Our structures serve as a nucleation point for the design of further structure-based experiments to probe the biochemical and biomedical roles of these proteins. III. General structure and biochemistry II. Background and Significance CATH 3.40.630.10 proteins are distributed across 8 PFAM families, which form the large peptidase_MH clan (CL0035). Also known in the MEROPS peptidase database as the clan MH/MC/MF of metallopeptidases: PF04952 Succinylglutamate desuccinylase / Aspartoacylase family (AstE-AspA ) 458 proteins PF02127 Aminopeptidase I Zinc metalloprotease M18 227 1 JCSG structures, 5 all other SG 4 all other SG PF01546 Peptidase family M20/M25/M40 3779 3 JCSG structures, 7 all other SG 6 non-SG PF00246 Zinc carboxypeptidase M14 1013 2 JCSG structures 10 non-SG PF04389 Peptidase family M28 812 PF00883 Cytosol aminopeptidase family, catalytic domain 827 1 all other SG 1 non-SG PF05343 M42 Glutamyl aminopeptidase 427 1 JCSG structures, 1 all other SG 1 non-SG PF05450 Nicastrin (eukaryotic, not known to be peptidase, part of γsecretase complex, no structures) 48 None None 5 non-SG These proteins are involved in a variety of proteolytic activities, have a range of substrate specificities and are present in numerous microbial organisms, many of which are important human pathogens like S. aureus, S. typhimurium, T. vaginalis, M. tuberculosis, N. gonorrhea, N. meningitidis, C. trachomatis, G. intestinalis, and E. coli. Several of these proteins have been investigated for their therapeutic potential and diseases roles (Canavan’s disease, cancer therapy and prohormone/propeptide processing). IV. Progress of structure determination These metallopeptidases show a high degree of structural conservation in the CATH domain which has a α/β/α sandwich architecture. The active site usually comprises of histidines and carboxylates interacting with two zinc ions. Despite the variety of molecular functions and substrate specificities of these proteins, the catalysis most likely involves a hydroxyl ion ligand involved in a nucleophilic attack. The full proteins often oligomerize and display some differences in their oligomerization state, however, the exact role of the oligomer in the molecular function is still unclear. In some cases, dimer formation results in assembly of a productive catalytic site. Dimerization is usually mediated by a dimerization domain. Higher oligomeric forms such as tetramers or octamers are also observed for some proteins. Distribution of selected targets across Pfam families Current status of 137 targets 140 120 Targets assigned in PfamA Targets unassigned in PfamA * 100 PF04952 32 3 80 60 PF02127 0 0 40 PF01546 56 1 All targets selected in March 2007 20 0 PF00246 9 8 PF04389 10 7** PF00883 2 0 PF05343 5 1 PF05450 0 7** Selected Cloned Expressed Purified Crystallized Diffracted Solved PDB Deposit * PFAM assigned based on sequence homology detected with FFAS http://ffas.ljcrf.edu/ffas-cgi/cgi/ffas.pl There are 3 targets not assigned by PfamA or FFAS. ** 7 targets indicated show significant FFAS match to both PF04389 and PF05450, and could possibly be distant bacterial homologs to the exclusively eukaryotic nicastrin family (PF05450). Figure of the representative CATH structure fro http://cathwww.biochem.ucl.ac.uk/cgi-bin/cath/GotoCath.pl?cath=3.40.630.10 V. Structures solved by JCSG 2QJ8.pdb (HP10622H), 2.0Å, R/Rf= 20.7/25.4%, Unknown function, PF04952 Homolog involved in Canavan’s disease 2QVP.pdb (HP10645A), 2.0Å, R/Rf= 16.1/21.3% Unknown function, PF04952 Structure suggests target may be closer in homology To PF00246 family VI. Phylogenetic tree and structure tree 3B2Y.pdb (HP10645E), 1.74Å, R/Rfr=17.45/21.51% Unknown function, PF04952, Ni+2 bound Structure suggests target may be closer in homology To PF00246 family 2RB7.pdb (HP1666A), 1.6Å, R/Rfr=15.4/18.0% Unknown function, PF01546 48 close homologs from important human pathogens Potential in cancer therapy VIII. Comparison of two proteins with >30% sequence identity within the same Pfam PF01546: 1CG2, 2RB7 Sequence with >30% identity within a particular Pfam also cluster together in structure space Target was selected based on 30% sequence id over full length protein and despite similairty in CATH domain, more diversity is observed in the dimerization domain. 2QYV.pdb (HP9625C), 2.11Å, R/Rf= 22.0, 24.4% Putative Xaa-His dipeptidase, PF01546, Zn+2 bound 7 close homologs from important human pathogens XI. Elucidation of a unique oligomeric form 2RB7 (cyan) and 1CG2, PF01546. Proteins in this Pfam with solved structures and >30% seq id with one another have functions which include succinyl-diaminopimelate desuccinylase activity; Carboxypeptidase G2 which cleaves C-terminal glutamate moiety from folic acid and its analogues, such as methotrexate; N-acetyl-L-citrulline deacetylase and Peptidase T tripeptidase. The 2QYV (PepD, MEROPS M20.007, clan MH, subfamily C) monomer is very similar in structure to the 1LFW monomer (PepV, MEROPS M20.004, subfamily A). Both are dipeptidases belonging to PF01546. However, 1LFW is known to function as a monomer in which the molecular structure mimics that of a dimer seen in most other proteins in this Pfam. PepD in E. coli and PrevotellaAcknowledgements albensis are seen to function as dimers. 2QYV represents the first crystal structure of a PepD, revealing it to be dimeric in the crystal structure (monomers in magenta and gold) as well as by size exclusion chromatography and shows the structural nature of the dimer. This novel structure serves as a starting point for further experiments to probe the effect of this unique dimer formation on protein function. Based on this information, it would now be possible to perform targeted biochemical assays to determine substrate for 2RB7, to try to understand the structural basis for substrate selection and specificity and to exploit this information for its therapeutic potential. For example, can 2RB7 hydrolyse methotrexate? Can it do so more efficiently? Can active site engineering based on structural information produce a more potent enzyme? Active site in 2RB7 1CG2:C-terminal glutamate moiety from folic acid and its analogues, such as methotrexate Common core ~290 aa, RMSD ~3.0 Å http://fatcat.burnham.org/POSA For structures that cluster together at 30% level, structural conservation in the common core is the highest, Generally only slight rearrangement of secondary structural elements is observed (within the domain). VII. Suggestion of PfamA assignment based on structure IX. Proteins with <30% sequence id. within the same Pfam HP10645A (2QVP) and HP10645E (3B2Y) sequences are assigned to PF04952 in PfamB. However, structural comparisons of the CATH domain show a stronger similarity to a member of PF00246 (1QMU, left) than to a member of PF04952 (2QJ8, center). This is also supported by structure & phylogenetic trees and FFAS. Also, like 1QMU, HP10645A/E lacks an ~70 amino acid insertion that forms a “C-terminal domain” (right, black circle), which is present in all PF04952 proteins and is important for biochemical function. These two pieces of evidence suggest and support the assignment of HP10645A/E in PF00246 in PfamA. PF01546: 2RB7, 2QYV (green) Common core ~250 aa, RMSD ~3.0 Å HP10625B, 2.3Å, work in progress PF01546 50 close homologs from important human pathogens Potential in cancer therapy X. Active site study may lead to structural basis of substrate specificity 2RB7: Unknown function, JCSG http://www.phlogeny.fr 2FVG.pdb (TM1049), 2.01Å, R/Rf= 20.3/24.4% Endoglucanase, PF05343 27 close homologs from important human pathogens Active site is 1CG2 is H112, D141, E200, E176, H385 Based on this, putative active site in 2RB7 is H72, D99, D100, E138, E139, D162 Hydrolysis of methotrexate by 1CG2 XII. Inferences and further work •In the quest for increasing structural coverage across protein families, it is expected that proteins similar in sequence within a protein family will be similar in structure. Increasing structural coverage provides better templates for modeling other proteins. The comparative structural analysis presented here provides experimental verification of the validity of this approach. •The structures for the proteins HP10645A and HP10645E suggest that they should be assigned to PF00246 in PfamA instead of the current suggestion of belonging to PF04952 by PfamB. • The 7 structures presented here provide a basis for enhancing the modeling of 2177 out of 7591 proteins (~29%) belonging to this Pfam clan. Furthermore, 3 of these JCSG structures provide the first examples of structures for proteins within a particular sequence cluster (2QYV, 2QJ8 and 3B2Y) and thus provide the basis for modeling 384 unique proteins (10 from organisms listed as top human pathogens) belonging to these 3 clusters from 2 different Pfams (PF01546 and PF04952). Common core of 226 aa, RMSD 2.45 Å Common core of 191 aa, RMSD 2.49 Å GNF & TSRI (Crystallomics Core) UCSD & Burnham (Bioinformatics Core) John Wooley Lukasz Jaroszewski Lian Duan Natasha Sefcovic Andrew Morse Tamara Astakhova Cindy Cook Adam Godzik Slawomir Grzechnik Sri Krishna Subramanian Piotr Kozbial Prasad Burra Josie Alaoen Dana Weekes Superimposition of all 6 structures in PF04952: 1YW4, 1YW6, 2BCO, 2G9D, 2GU2 and 2QJ8 Scott Lesley Dennis Carlton Marc Deller Polat Abdubek Julie Feuerhelm Hope Johnson Sebastian Sudek Glen Spraggon Charlene Cho Jessica Canseco Mark Knuth Heath Klock Thomas Clayton Kevin D. Murphy Daniel McMullan Christina Trout Claire Acosta Linda M. Columbus Joanna C. Hale Thamara Janaratne Linda Okach Edward Nigoghossian Aprilfawn White Bernhard Geierstanger Ylva Elias Sanjay Agarwalla Bi-Ying YehAnna Grzechnik Mimmi Brown Larger rearrangements and extensions of secondary structural elements. Inserts and novel features more common. Common core ~190 aa, RMSD ~3.0 Å PF04952: 2QJ8, 3B2Y (cyan) • 2QYV/HP9625C represents the first crystal structure of a dipeptidase PepD showing a dimer. • Further analysis will be performed to try to understand evolutionary relationships between these proteins based on sequence-based phylogenetic trees and structure-based trees. • Attempts will be made to investigate use of these structures and their comparative analyses in understanding structural basis for enzyme function and substrate specificities by analysis of active site amino acids, and to attempt to exploit information for therapeutic purposes. Stanford /SSRL (Structure Determination Core) Keith Hodgson Mitchell Miller Hsiu-Ju (Jessica) Chiu Christopher Rife Silvya Oommachen Henry van den Bedem Christine Trame Ashley Deacon Debanu Das Kevin Jin Qingping Xu Scott Talafuse Ronald Reyes TSRI (NMR Core) Kurt Wüthrich Reto Horst Maggie Johnson Amaranth Chatterjee Michael Geralt Wojtek Augustyniak Pedro Serrano Bill Pedrini William Placzek TSRI (Admin Core) Ian Wilson Marc Elsliger Gye Won Han David Marciano Henry Tien Xiaoping Dai Lisa van Veen Scientific Advisory Board Sir Tom Blundell Univ. Cambridge Homme Hellinga Duke University Medical Center James Naismith The Scottish Structural Proteomics facility Univ. St. Andrews Soichi Wakatsuki Photon Factory, KEK, Japan James Wells UC San Francisco Robert Stroud Center for Structure of Membrane Proteins Membrane Protein Expression Center UC San Francisco James Paulson Consortium for Functional Glycomics The Scripps Research Institute Todd Yeates UCLA-DOE Inst. for Genomics and Proteomics The JCSG is supported by the NIH Protein Structure Initiative (PSI) Grant U54 GM074898 from NIGMS (www.nigms.nih.gov). Portions of this research were carried out at the Stanford Synchrotron Radiation Laboratory (SSRL). The SSRL is a national user facility operated by Stanford University on behalf of the U.S. Department of Energy, Office of Basic Energy Sciences. The SSRL Structural Molecular Biology Program is supported by the Department of Energy, Office of Biological and Environmental Research, and by the NIH. Annual meeting with SAB 2007