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Download BACTERIA & VIRUSES - Bishop Shanahan High School
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Prokaryotes & Viruses PROKARYOTES SINGLE CELLED; WITHOUT NUCLEUS 1 – 5 MICROMETERS IN SIZE ARCHAEBACTERIA LACK PEPTIDOGLYCAN IN CELL WALL LIPIDS IN CELL MEMBRANE DIFFERENT DNA SEQUENCES HAVE INTRONS – LIKE EUKARYOTES EUBACTERIA (BACTERIA) GREAT VARIETY; INCLUDES MOST BACTERIA LIVE ALMOST EVERYWHERE! CELL WALL: PEPTIDOGLYCAN SOME HAVE CAPSULE; PILI SHAPES OF BACTERIA BACILLUS – ROD SHAPED COCCUS – SPHERICAL SPIRRILLUM – SPIRAL CHAINS: Strepto-; CLUSTERS: STAPHO- CELL WALL GRAM STAIN: 2 dyes – violet (main); red Gram +: cell wall thicker; mostly peptidoglycan; absorb violet stain Gram -: second layer of lipid & carbohydrate; absorbs red; more resistant to antibiotics CAPSULE MANY HAVE ONE; SURROUNDS CELL WALL POLYSACCHARIDES; PROTECTS CELL FROM DRYING OUT IF FUZZY: glycocalyx – allows cell to stick to host cell or substrate PILI SHORT EXTENSIONS ON SOME ADHERENCE TO HOST; SOME USED TO TRANSFER GENES FROM ONE CELL TO ANOTHER NUTRITION (GETTING ENERGY) AUTOTROPHIC Photoautotrophs Cyanobacteria (in jellylike covering). Heterocysts - N fixation Phycocyanin & Chl a AUTOTROPHS (CONTD) Chemoautotrophs – Use inorganic chemical energy to make organic molecules – Gram – bacteria HETEROTROPHS MOST DECOMPOSERS/SAPROPHYTES ENTERIC: Gram - inhabit animal intestine. E. coli – Produces Vitamin K RELEASING ENERGY AEROBIC ANAEROBIC – Clostridium botulinum FACULTATIVE ANAEROBE GROWTH & REPRODUCTION GROW AND REPRODUCE RAPIDLY BINARY FISSION: no genetic exchange or recombination; asexual CONJUGATION: hollow bridge forms between organisms; genetic exchange ENDOSPORE DORMANT FORM MAY FORM IN UNFAVORABLE CONDITONS THICK WALLED; ENCLOSES CYTOPLASM & DNA NOT FORM OF REPRODUCTION! ROLE IN NATURE DECOMPOSERS – MAJOR ROLE! Recycle nutrients Breakdown of dead matter Important in sewage treatment plants: organic matter broken down can be reused as fertilizer ROLE IN NATURE NITROGEN FIXATION Nitrogen need to make amino acids for protein Air: contains 79% nitrogen Plants can’t use N2 directly Bacteria “fix” Nitrogen into NH3 or NO3 Rhizobium – Nitrogen fixing bacteria in root nodules of legumes ROLE IN NATURE DISEASE CAUSING: Pathogenic Damage tissues directly or release toxins (Streptococcus) Antibiotics: Block growth and reproduction of cell; breakdown or weaken cell wall POSITIVE USES CHEESE; YOGURT; BUTTERMILK; SOUR CREAM CLEAN UP OIL SPILLS MINE MINERALS SYNTHESIZE DRUGS IN GENETIC ENGINEERING VIRUSES Tobacco Mosaic Virus – first isolated Subcellular; Obligate Intracellular parasite Can pass through a filter; can be crystallized STRUCTURE OF VIRUS NUCLEIC ACID CORE SURROUNDED BY A PROTEIN COAT (CAPSID) NUCLEIC ACID: EITHER DNA OR RNA SOME HAVE ENVELOPE: derived from host cell membrane during replication (flu; HIV; Chicken pox) CLASSIFICATION OF VIRUSES CLASSIFIED BY ORGANISM THEY INFECT VIRUSES ARE SPECIFIC TO WHAT THEY INFECT BACTERIOPHAGE: infect bacteria; DNA VIRAL REPLICATION LYTIC CYCLE – Virus enters the cell; makes viral copies; cell bursts and is destroyed LYSOGENIC CYCLE- Viral DNA becomes embedded into DNA of host and replicates with host DNA. Cell is not destroyed LYTIC CYCLE: VIRULENT VIRUS 5 STAGES: ATTACHMENT ENTRY OF NUCLEIC ACID REPLICATION OF VIRAL PARTICLES ASSEMBLY RELEASE: Lysis of cell LYSOGENIC CYCLE: TEMPERATE VIRUS ATTACHMENT INJECTION OF NUCLEIC ACID INTEGRATION INTO BACTERIAL CHROMOSOME (prophage) CELL MULTIPLICATION VIRAL DISEASES POLIO; MEASLES; AIDS; MUMPS; FLU; YELLOW FEVER; COLD SOME VIRUSES ARE ASSOCIATED WITH CANCER VACCINE: preparation of weakened or killed virus RETROVIRUS RNA CONTAINING VIRUS RNA DNA uses enzyme: reverse transcriptase DNA produced from RNA is inserted into host cell DNA: PROVIRUS HIV PRIONS PROTEIN PARTICLE CAPABLE OF CAUSING DISEASE ABNORMAL FORM OF PROTEIN THAT CLUMPS INSIDE A CELL; CLUMPING KILLS CELL MAD COW DISEASE; SCRAPIE IN SHEEP (nervous system degeneration) VIROIDS SMALLEST PARTICLES ABLE TO REPLICATE SHORT, SINGLE STRAND OF RNA WITHOUT CAPSID HARMFUL TO PLANTS ORIGIN OF VIRUSES? Many believe viruses evolved from early cells First viruses may have been naked nucleic acid that could move from cell to cell Derived from degenerated form of evolution