Download Micrococcus luteus, rhodospirillum rubrum, and

Second Mountain Park Elementary Conference on Cell Biology, October 2013
irst and last) for
esentations.
Bacterial Growth
Hi kids. Keep up the good w
I made some comments a
corrections for you. Correcti
are mostly in red. I hope th
helps. – Dr. Knotts
Carter, Braeden, Maxwell, and Giles
Our group is G5F1
Mustang
Ms. Moffitt’s Class
Science Lab
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Second Mountain Park Elementary Conference on Cell Biology, October 2013
Our EQs
EQ 1
What does bacteria grow best on?
EQ 2
Can bacteria growth be harmful?
EQ 3 How is bacteria growth influenced by the
concentrations of the nitrousnutrients in the substrate?
EQ 4
Can you use a aseptic culture procedures to
measure the population growth of bacteria?
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Second Mountain Park Elementary Conference on Cell Biology, October 2013
How to cultivate bacteria
These things are used to prepare specimens
for viewing under the microscope
You need to get amber bottles,
cover slips, 1% crystal violet
stain, disposable pipettes,
disposable lighter, gram iodine
solution, metric ruler,
microscope slides, 1% safranin
stain, screw cap plastic
centrifuge vials, you will also
need halobacteriaum, a triple
bacteria, a standard transmitted
light microscope scale, and a
pipettor These things are use to culture –
another name for grow – bacteria.
why?
Name the bacteria you studied:
Halobacterium NRC-1 and a triple species
mix consisting of Micrococcus luteus,
Bacillus subtilis, and Rhodospirillum rubrum
3
d the triple species mix, not
cterium, for this experiment.
k me – maybe I’m wrong.
Second Mountain Park Elementary Conference on Cell Biology, October 2013
Summary of what we did
We added halo bacteria to tubes filled with 10 ml of nutrient broth
containing 1% sugar, 64%sugar, 6412% salt, and nutrient brothno
added salt or sugar. Then our group added the solutions to plates of
nutrient agar using the pippetor (and pipettes).In three weeks the
64%sugar did not have many bacteria colonies. The 6412% salt did
not contain many colonies either. The nutrient broth had many
bacteria colonies, though it did not have as much bacteria as the 1%
sugar. We did the same experiment with a triple mixture of bacteria
(Micrococcus luteus, rhodospirillum rubrum, and Bacillus subtilis).
ents not described in
say something about
pared Halobacterium
cies mix using agar in
nutrient agar and
aining a large quantity
olved serial dilutions
h rates at different
ures.
You let the
allow time
used the
Italicize the scienti
of organisms such
bacteria spec
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Second Mountain Park Elementary Conference on Cell Biology, October 2013
Lesson Review
1 What does bacteria grow best on?
1 In this experiment we learned that bacteria grows best on 1% sugar and nutrient broth
but it grows the least 64% sugar and salt. It grows best in warm temperatures such as 16
degrees.
2 Can bacteria be harmful?
2 Yes bacteria can be harmful but not all bacteria can for example frozen yogurt is full of
bacteria. It is also frozen alive!
3 How is bacteria growth influenced by the concentrations of the nitrous in the substrate?
3 Yes it is affected the bacteria because the need oxygen and Glucose molecules to use in
there mitochondria because large nutrient molecules cant just pass through the cell
membrane.
4
You can use a aseptic culture to produce bacteria.
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Second Mountain Park Elementary Conference on Cell Biology, October 2013
Words to know
Bacteria
Aseptic technique
Bacterial suspension
Broth cultures
Colony counting
Nutrient agar
Salt sensitive
Salt resistant
Serial dilutions
Substrate
Triple mixture
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