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Second Mountain Park Elementary Conference on Cell Biology, October 2013 irst and last) for esentations. Bacterial Growth Hi kids. Keep up the good w I made some comments a corrections for you. Correcti are mostly in red. I hope th helps. – Dr. Knotts Carter, Braeden, Maxwell, and Giles Our group is G5F1 Mustang Ms. Moffitt’s Class Science Lab 1 Second Mountain Park Elementary Conference on Cell Biology, October 2013 Our EQs EQ 1 What does bacteria grow best on? EQ 2 Can bacteria growth be harmful? EQ 3 How is bacteria growth influenced by the concentrations of the nitrousnutrients in the substrate? EQ 4 Can you use a aseptic culture procedures to measure the population growth of bacteria? 2 Second Mountain Park Elementary Conference on Cell Biology, October 2013 How to cultivate bacteria These things are used to prepare specimens for viewing under the microscope You need to get amber bottles, cover slips, 1% crystal violet stain, disposable pipettes, disposable lighter, gram iodine solution, metric ruler, microscope slides, 1% safranin stain, screw cap plastic centrifuge vials, you will also need halobacteriaum, a triple bacteria, a standard transmitted light microscope scale, and a pipettor These things are use to culture – another name for grow – bacteria. why? Name the bacteria you studied: Halobacterium NRC-1 and a triple species mix consisting of Micrococcus luteus, Bacillus subtilis, and Rhodospirillum rubrum 3 d the triple species mix, not cterium, for this experiment. k me – maybe I’m wrong. Second Mountain Park Elementary Conference on Cell Biology, October 2013 Summary of what we did We added halo bacteria to tubes filled with 10 ml of nutrient broth containing 1% sugar, 64%sugar, 6412% salt, and nutrient brothno added salt or sugar. Then our group added the solutions to plates of nutrient agar using the pippetor (and pipettes).In three weeks the 64%sugar did not have many bacteria colonies. The 6412% salt did not contain many colonies either. The nutrient broth had many bacteria colonies, though it did not have as much bacteria as the 1% sugar. We did the same experiment with a triple mixture of bacteria (Micrococcus luteus, rhodospirillum rubrum, and Bacillus subtilis). ents not described in say something about pared Halobacterium cies mix using agar in nutrient agar and aining a large quantity olved serial dilutions h rates at different ures. You let the allow time used the Italicize the scienti of organisms such bacteria spec 4 Second Mountain Park Elementary Conference on Cell Biology, October 2013 Lesson Review 1 What does bacteria grow best on? 1 In this experiment we learned that bacteria grows best on 1% sugar and nutrient broth but it grows the least 64% sugar and salt. It grows best in warm temperatures such as 16 degrees. 2 Can bacteria be harmful? 2 Yes bacteria can be harmful but not all bacteria can for example frozen yogurt is full of bacteria. It is also frozen alive! 3 How is bacteria growth influenced by the concentrations of the nitrous in the substrate? 3 Yes it is affected the bacteria because the need oxygen and Glucose molecules to use in there mitochondria because large nutrient molecules cant just pass through the cell membrane. 4 You can use a aseptic culture to produce bacteria. 5 Second Mountain Park Elementary Conference on Cell Biology, October 2013 Words to know Bacteria Aseptic technique Bacterial suspension Broth cultures Colony counting Nutrient agar Salt sensitive Salt resistant Serial dilutions Substrate Triple mixture 6