Download Autoantibody-Mediated Dysfunction of Sympathetic Neurons in

From: Autoantibody-Mediated Dysfunction of Sympathetic Neurons in Guillain-Barré Syndrome
Arch Neurol. 2010;67(2):203-210. doi:10.1001/archneurol.2009.331
Figure Legend:
Staining properties, cell viability, and noradrenaline synthesis of sympathetic neurons during treatment with IgG from patients with
Guillain-Barré syndrome (GBS) and control patients. A, Staining of sympathetic neurons with GBS-IgG (green) and antibody against
tyrosine hydroxylase (red, bar = 50 μm). Cell nuclei are labeled with 4′,6-diamidino-2-phenylindol (DAPI). The IgG from controls and
patients with GBS stained predominantly the cell bodies of rodent sympathetic neurons (see the Table for summary of staining
pattern). B, Survival of sympathetic neurons in the
presence
of different
human
IgG. Treatment with GBS-IgG or control IgG does
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American
Medical
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cell viability after 24 hours, as determined
by
fluorescence-based
cell viability assay. C, Average change of
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noradrenaline levels in supernatants of sympathetic neurons 24 hours after treatment with GBS-IgG or control IgG. Noradrenaline
From: Autoantibody-Mediated Dysfunction of Sympathetic Neurons in Guillain-Barré Syndrome
Arch Neurol. 2010;67(2):203-210. doi:10.1001/archneurol.2009.331
Figure Legend:
Guillain-Barré syndrome (GBS)-IgG and the beat rate of cardiomyocytes innervated by sympathetic neurons. A, Cocultures of
sympathetic neurons and cardiomyocytes stained against tyrosine hydroxylase (red) and α-actinin (green), a marker to visualize
cardiomyocytes (bar = 250 μm). B, Staining against tyrosine hydroxylase (green) and synaptophysin (red), a presynaptic marker for
synaptic vesicle glycoprotein confirms synapse formation in cocultures of sympathetic neurons and cardiomyocytes (indirectly
labeled with 4′,6-diamidino-2-phenylindol [bar = Copyright
50 μm]). C,
Fluorescence-staining
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American Medical (left) and light microscopy (right) of a
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5/12/2017
representative
cluster
of cardiomyocytes and innervating
sympathetic
Association. All rights neurons.
reserved. Cells are stained with antibodies against tyrosine
hydroxylase (red) and α-actinin (green) (bar = 100 μm). Arrowheads indicate the cell bodies of innervating sympathetic neurons. (A
From: Autoantibody-Mediated Dysfunction of Sympathetic Neurons in Guillain-Barré Syndrome
Arch Neurol. 2010;67(2):203-210. doi:10.1001/archneurol.2009.331
Figure Legend:
Tyrosine hydroxylase expression of sympathetic neurons. A, Mean expression of tyrosine hydroxylase messenger RNA (mRNA) in
sympathetic neurons in the presence of different IgG and after treatment with intravenous immunoglobulins (IVIg). Guillain-Barré
syndrome (GBS)-IgG increased the expression of tyrosine hydroxylase mRNA in sympathetic neurons. Prior treatment of cells with
IVIg prevented the increase of tyrosine hydroxylase mRNA (IVIg and GBS-IgG), in contrast to preincubation of IVIg and GBS-IgG
before cell exposure. B, Western blot of cell lysates
revealed
increased
tyrosine
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American
Medicalhydroxylase expression in sympathetic neurons
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exposed
to GBS-IgG.
C, Densitometric analysis ofAssociation.
Western blot
tyrosine
hydroxylase
bands in relation to protein concentration as
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determined by actin band intensity confirms mRNA data. Error bars indicate standard error of the mean; *P < .05; AU, arbitrary units;