Download Androgen Receptor (D6F11) XP® Rabbit mAb

Store at -20°C
Androgen Receptor (D6F11)
XP® Rabbit mAb
#5153
Orders n 877-616-CELL (2355)
[email protected]
Support n 877-678-TECH (8324)
[email protected]
Web n www.cellsignal.com
rev. 01/28/16
For Research Use Only. Not For Use In Diagnostic Procedures.
Entrez-Gene ID #367
UniProt ID #P10275
Applications
Species Cross-Reactivity*
Molecular Wt.
Isotype
W, IHC-P, IF-IC, F
Endogenous
H
110 kDa
Rabbit IgG**
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
**Anti-rabbit secondary antibodies must be used to
detect this antibody.
Background: Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily,
is activated by phosphorylation and dimerization upon
ligand binding (1). This promotes nuclear localization
and binding of AR to androgen response elements in the
androgen target genes. AR plays a crucial role in several
stages of male development and the progression of prostate
cancer (2,3).
Recommended Antibody Dilutions:
Western blotting
1:2000
Immunohistochemistry (Paraffin)
1:400†
Unmasking buffer: Citrate
Antibody diluent: SignalStain® Antibody Diluent #8112
Detection reagent: SignalStain® Boost (HRP, Rabbit) #8114
†Optimal IHC dilutions determined using SignalStain® Boost IHC
Detection Reagent.
Immunofluorescence (IF-IC)1:600
Flow Cytometry
1:400
Specificity/Sensitivity: Androgen Receptor (D6F11)
XP® Rabbit mAb detects endogenous levels of total androgen receptor protein.
Source/Purification: Monoclonal antibody is produced
by immunizing animals with recombinant protein corresponding to residues near the amino terminal region of
human androgen receptor protein.
For application specific protocols please see the web
page for this product at www.cellsignal.com.
Please visit www.cellsignal.com for a complete listing
of recommended companion products.
Background References:
(1) Li, J. and Al-Azzawi, F. (2009) Maturitas 63, 142-8.
(2) Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol.
76, 135-142.
kDa
200
140
LN
Ca
P
M
CF
7
PC
-3
DU
14
5
© 2015 Cell Signaling Technology, Inc.
XP, SignalStain and Cell Signaling Technology are trademarks of Cell Signaling Technology, Inc.
(3) Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.
Confocal immunofluorescent analysis of LNCaP (positive,
upper) and DU145 (negative, lower) cells using Androgen
Receptor (D6F11) XP® Rabbit mAb (green). Actin filaments have
been labeled with DyLight™ 554 Phalloidin #13054 (red).
Androgen
Receptor
100
80
60
50
40
200
140
100
80
60
50
β-Actin
40
Western blot analysis of extracts from LNCaP (AR+), MCF7
(AR+), PC-3 (AR-), and DU 145 (AR-) cells using Androgen Receptor (D6F11) XP® Rabbit mAb (upper) and β-Actin Antibody
#4967 (lower).
30
20
Applications Key:
W—Western
Species Cross-Reactivity Key:
IP—Immunoprecipitation
H—human
M—mouse
Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans
IHC—Immunohistochemistry
R—rat
Hr—horse
Hm—hamster
ChIP—Chromatin Immunoprecipitation
Mk—monkey
Mi—mink
All—all species expected
C—chicken
IMPORTANT: For western blots, incubate membrane
with diluted antibody in 5% w/v nonfat dry milk, 1X
TBS, 0.1% Tween®20 at 4°C with gentle shaking,
overnight.
DyLight is a trademark of Thermo Fisher Scientific Inc. and its
subsidiaries.
Tween is a registered trademark of ICI Americas, Inc.
IF—Immunofluorescence
F—Flow cytometry
Dm—D. melanogaster X—Xenopus
Z—zebrafish
E-P—ELISA-Peptide
B—bovine
Species enclosed in parentheses are predicted to react based on 100% homology.
Immunohistochemical analysis of paraffin-embedded human
prostate carcinoma using Androgen Receptor (D6F11) XP®
Rabbit mAb.
Events
Immunohistochemical analysis of paraffin-embedded LNCaP (AR+, left) and DU145 (AR-, right) using Androgen Receptor (D6F11)
XP® Rabbit mAb.
Androgen Receptor
® 2015 Cell Signaling Technology, Inc.
Flow cytometric analysis of DU-145 cells (red) and LNCaP cells
(blue) using Androgen Receptor (D6F11) XP® Rabbit mAb.
Orders n 877-616-CELL (2355)
[email protected]
Support n 877-678-TECH (8324)
[email protected]
Web n www.cellsignal.com