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Transcript
Genetic Engineering Videos Powerpoint embedded videos: DNA probe (McGraw/Hill animation, 2:00) https://highered.mheducation.com/sites/9834092339/student_view0/chapter17/dna_probe__dna_hybridizat ion_.html Genetic Engineering (7:20 min, history, restriction enzymes cutting paper, frog DNA & plasmid) • https://www.youtube.com/watch?v=nfC689ElUVk PBS Transforming Bacteria (2:35, P- LB/amp, P+ LB/amp & P+ LB/amp/ara plates shown) • https://ca.pbslearningmedia.org/resource/biot11.sci.life.gen.transbact/transforming-bacteria/ Transgenic Organisms (10:02 minutes, voiced over ppt, steps of make transgenic organisms-large hand human, antibiotic resistant marker, restriction enzymes, ) • https://www.youtube.com/watch?v=RqQi7mR8C6o Spider goats (2:39): • http://science.discovery.com/videos/kapow-superhero-science-spider-silk-gene-goats.html The Science of Transgenic Technology Part 1 of 2 (HHMI, transgenic mice with human disease genes by microinjection or forced crossing over 5:00) • https://www.youtube.com/watch?v=ujZHrR1mro8 Part 2 of 2 (6:07 min, transgenic mice creation, lots of detail) • https://www.youtube.com/watch?v=EFNtTfhhQ70 Other Good Videos: The Micropipette and Micropipetting Technique (review, 3 wrences? Otherwise good, 3:08) https://www.youtube.com/watch?v=FE676kkvH-g How to use a Micropipette (University lab, 1st & 2nd stops, new tips each time, check setting, 2:20) https://www.youtube.com/watch?v=8Afh_0IAfrQ Mastering Micropipettes (University of Colorado, Biology lab, setting volume reveiw, adding tip, 1st & 2nd stops, proper time to release plunger, 3:30) https://www.youtube.com/watch?v=wcLfqKnAE-k Laboratory Techniques: Using A Micropipette (review of diff sizes, showing ranges for each, uses same pipette we use, safe use review, tips, 1st & 2nd stops, 5:26) https://www.youtube.com/watch?v=4eFlUuPGxSk Genetic Engineering (walk thru lab, history& overview, restriction enzymes, plasmids, how recombinated plasmids are made, e.coli plasmid insertion with heat shocking, growing e.coli on different media plates, 7:20) https://www.youtube.com/watch?v=nfC689ElUVk What is Genetic Engineering? (good overview, starts with DNA, uses creation of humulin as e.g. 2:32) https://www.youtube.com/watch?v=3IsQ92KiBwM Genetic Engineering-Ann Johnson (universal genetic code, restriction & ligase enzymes, e.g. of diff. restriction enzymes making sticky ends, good overview before using recombinated plasmid, shows how these are made, bacteria diagram with its DNA + plasmids, 9:24) https://www.youtube.com/watch?v=BK12dQq4sJw Glowing Rats and Extreme Genetic Engineering (synthetic biology, 2010 started, inserting created genes into organisms, discussion of safety, moral issues, etc.4:08) https://www.youtube.com/watch?v=k7-ofCWJiUg Genetic Engineering Animation (9th grader created, overviews genes, restriction enzymes cutting genes, adding into plasmids, 3:04) https://www.youtube.com/watch?v=AEINuCL-5wc Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls (how to tell which bacteria got the right plasmid, antibiotic resistance, control groups,7:44)-watch as much as you can, sign in if you want http://study.com/academy/lesson/bacterial-transformation-screening-and-interpreting-experimentalresults.html Bacterial Transformation: Definition & Process of Genetic Engineering of E.coli (making competent cells, focus on neutralizing negative cell membrane & DNA charge, heat shock)-watch as much as you can http://study.com/academy/lesson/bacterial-transformation-definition-process-and-genetic-engineeringof-e-coli.html Bacterial Transformation: Definitions, steps & analysis (starts with mother/daughter bacterial cell conversation, ~6) -watch as much as you can, sign in if you want http://study.com/academy/lesson/bacterial-transformation-definition-steps-analysis.html Genetic Engineering https://www.youtube.com/watch?v=gIgV_VQpv7s GCSE Biology - Genetic Engineering Insulin (restriction enzymes cutting out desired gene, adding it to plasmid, using electric shock to insert into bacteria, 2:12) https://www.youtube.com/watch?v=H7FdzpE2GIE Molecular Biology, Bozeman Bio (Yellowstone part enzyme connection, restriction enzymes cut, hydrogen bonds recombine, ligase “re-tapes”, genetic markers, restriction enzymes to protect bacteria against viruses, uses scissors for enzymes, glue for H-bonds, game analogy for electrophoresis with DNA ladder, PCR, 14:32) https://www.youtube.com/watch?v=yYIZgS-L5Sc DNA Fingerprinting, Bozeman Biology (University of Leicester, non-coding regions, Short tandom repeats, introns, Mr. Blond, Ms. Red & Mr. Mustache, 6:09) https://www.youtube.com/watch?v=DbR9xMXuK7c&t=2s Genetic Recombination and Gene Mapping (fruit fly chromosomes, crossing over, 9:49) https://www.youtube.com/watch?v=TU44tR0hJ8A Plasmids: Tools of Genetic Engineering (bacterial animation with restriction enzymes, antibiotic resistance, Eukaryotic DNA transformation, 4:14) https://www.youtube.com/watch?v=GNMJBMtKKWU Construction of a Plasmid Vector [HD Animation] (close up of plasmids & transforming process, restriction enzymes, lac Z gene in vectors, 2:02) https://www.youtube.com/watch?v=KRpik9mNRm0 Plasmids and Recombinant DNA Technology (lecture in front of whiteboard, plasmid review, 14:31) https://www.youtube.com/watch?v=2YxTcBimxlw Plasmid Rap (male & female students sing, 2:53) https://www.youtube.com/watch?v=QFfUFyUvP-w DNA - Science Rap Academy (2:48) https://www.youtube.com/watch?v=VrTGclugG0k