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Glycogen metabolism supports effector function and energy homeostasis of dendritic cells Phyu Thwe1, Saritha Beauchamp1, and Eyal Amiel1 Affiliation Medical Laboratory and Radiation Sciences, Immunobiology Department Background and Objective Dendritic cells (DCs), professional antigen presenting cells of the immune system, serve as a bridge between the innate and adaptive immune responses. Activation of DCs by a stimulus through toll-like receptors (TLRs) is coupled with an increase in energy demand fulfilled by a glycolytic burst, which provides DCs with molecular building blocks for their effector function. Inhibition of glycolysis impairs the survival and effector function of activated DCs. While non-immune cells such as hepatocytes are known to store glucose in the form of glycogen as intracellular energy reserve, the role of glycogen metabolism in DCs has not been studied. The purpose of this study is to understand the role and regulatory mechanisms of glycogen metabolism in DC effector function. Methods We employ immunological techniques such as flow cytometry and enzyme linked immunoabsorbent assays. We also use commercial biochemical assays such as glucose and glycogen assays. Results We show that DCs express the enzymes essential for glycogen metabolism and that glycogen metabolism is regulated upon TLR stimulation. We also demonstrate that inhibition of glycogen metabolism impairs activation of these cells. Discussion and Conclusions Our work suggests that glycogen-derived glucose carbons feed into the tricarboxylic acid cycle to support early maturation of DCs and that glycogen metabolism in DCs plays an important role to support their effector function.