Download Principal Investigator/Program Director (Last, First, Middle): Halonen

Principal Investigator/Program Director
(Last, First, Middle):
Halonen, Sandra K.
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors in the order listed on
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NAME
POSITION TITLE
Sandra K. Halonen
Associate Professor
eRA COMMONS USER NAME
SHALONEN
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing,
and include postdoctoral training.)
DEGREE
INSTITUTION AND LOCATION
(if
YEAR(s)
FIELD OF STUDY
applicable)
University of Illinois, Champaign-Urbana, IL.
B.S.
1976-1980 Biology
M.S.
University of Illinois, Champaign-Urbana,
IL.
1982-1984 Evolutionary Ecology
Champaign-Urbana.
M.S.
Ph.D.
Louisiana
University,
Baton Rouge, LA.
1989-1993
UniversityState
of Illinois,
Champaign-Urbana.
IL.
1982-1984 Parasitology
Evolutionary Ecology
A.
Personal
Statement
University
of Illinois,
Champaign-Urbana.
IL.
1982-1984
Louisiana State
University,
Baton Rouge, LA.
1989-1993 Parasitology
My
research
is
focused
on
understanding
the
role
of
astrocytes
in
the
intracerebral immune response to
Louisiana State University, Baton Rouge, LA.
1989-1993
T. gondii in latent infections in immunocompetant hosts and in reactivated Toxoplasmosis in immunosuppressed
individuals. Recently my research has begun to define the functional impact of the parasite in neurons and to
investigate chronic Cerebral Toxoplasmosis and development of neuropsychiatric disorders such as Schizophrenia.
B. Positions and Honors
Positions and Employment
1994-1997
Aaron Diamond Post Doctoral Research Fellow, Dept. of Neurology, Albert Einstein
College of Medicine, Bronx, NY
1997-1998
Research Associate, Dept. of Pathology, Albert Einstein College of Medicine,
Bronx, NY
1998-2003
Assistant Professor, Dept. of Natural Sciences, Mercy College, Dobbs Ferry, NY
1998-present Visiting Assistant Professor, Dept. of Pathology; Albert Einstein Coll. of Medicine,
Bronx, NY
2003-present Affiliate Assistant Professor, Dept. of Microbiology, University of Washington,
Seattle, WA
2003-2009
Assistant Professor, Dept. of Microbiology, Montana State University, Bozeman, MT
2010-present Associate Professor, Dept. of Microbiology and Immunology, Montana State
University, Bozeman, MT
Honors
1989-93
LSU Alumni Federation Fellowship
1994-97
Aaron Diamond Postdoctoral Fellowship, Albert Einstein College of Medicine;
Bronx, N.Y.
2001
Sr. Gratia Mahia Award for Excellence in Teaching, Mercy College, Dobbs Ferry NY
2008
Lucy Logan Research Award; Montana State University, Bozeman, MT.
2007-09
External Advisory Committee Member, NIH-Fogarty Global Infectious Disease
Training Grant; Albert Einstein College of Medicine; Bronx, N.Y
2011-present Associate Editor for Journal of Neuroinfectious Diseases
PHS 398/2590 (Rev. 09/04)
Page
1
Biographical Sketch Format Page
Principal Investigator/Program Director (Last, First, Middle):
Halonen, S.K.
C. Peer-Reviewed Publications (in chronological order)
Weidner, E. and S.K. Halonen. 1993. Microsporidian spore envelope keratins phosphorylate and
disassemble during spore activation. J. Euk. Microbiol. 40:783-788.
Halonen, S.K. and E.Weidner. 1994. Overcoating of Toxoplasma parasitophorous vacuoles with host cell
vimentin type intermediate filaments. J. Euk. Microbiol. 41:65-71.
Weidner, E., S.B. Manale, S.K. Halonen, and J.D. Lynn. 1994. Microsporidian spore invasion tubes as
revealed by fluorescent probles. Biol. Bull. 187:255-6.
Weidner, E., S.B. Manale, S.K. Halonen, and J.D. Lynn. 1995. Protein-membrane interaction is essential to
normal assembly of the microsporidian spore invasion tube. Biol Bull. 188: 128-135.
Halonen, S.K., E. Weidner and J.F. Siebenaller. 1996. Evidence for heterotrimeric GTP-binding proteins in
Toxoplasma gondii. J. Euk. Microbiol. 43:187-192.
Halonen, S.K., W.D. Lyman and F.C. Chiu. 1997. Growth and development of Toxoplasma gondii in a
human neurons and astrocytes. J. Neuropath. Exp. Neurology 55:1150-56.
Halonen, S.K., F.C. Chiu and L.M. Weiss. 1998. Association of host cell intermediate filaments with
Toxoplasma gondii cysts in murine astrocytes. Int. J. Parasitology 28:815-823.
Halonen, S.K., F.C. Chiu and L.M. Weiss. 1998. Effect of cytokines on the growth of Toxoplasma in
murine astrocytes. Infect. Immun. 66:4989-4993.
Ma Y.F., Y.W. Zhang, S.K. Halonen, M.M. McAllister, and L.M. Weiss. 1999. The in vitro development of
Neospora caninum bradyzoites. Int J. Parasitol. 10:1713-23.
Halonen, S.K. & L.M. Weiss. 1999. Effect of cytokines and quercetin on Toxoplasma gondii cyst induction
in murine astrocytes. J. Euk. Microbiol. 46(5):83S-84S.
Halonen, S.K. and L.M. Weiss. 2000. Investigation into the mechanism of IFN-γ mediated inhibition of
Toxoplasma gondii in murine astrocytes. Infect. Immun. 68:3426-3430.
Huang, H., S.B. Petkova, R.G. Pestell, B. Bouzahzah, J. Chan, H. Magazine, L.M. Weiss, G.J. Christ, P.
Lisanti, S.A. Douglas, V. Shtutin, S.K. Halonen, M. Wittner and H.B. Tanowitz. 2000.Trypanosoma
cruzi infection (Chagas’ disease) of mice causes activation of the mitogen-activated protein kinase
cascade and expression of endothelin-1 in the myocardium. J. Cardiovasc. Pharmacol. 36:S148-50.
Zhang, Y.W., S.K. Halonen, Y.F. Ma, M. Wittner and L.M. Weiss. 2001. Initial characterization of CST, a
Toxoplasma gondii cyst wall glycoprotein. Infect. & Immun. 69:501-507.
Halonen, S.K. and L.M. Weiss. 2001. Interferon gamma induced inhibition of Toxoplasma gondii is
mediated by IGTP. Infect. Immun. 69: 5573-6.
Halonen, S.K. 2004. World Class Parasites Vol. 8: Opportunistic Infections: Toxoplasma, Sarcocystis,
Microsporidia. Chapter 5. Immune response to Toxoplasma gondii in the central nervous system.
David S. Lindsey and Louis M. Weiss Editors. Kluwer Academic Publishers; pp 67-88.
Suzuki, Y. and S.K. Halonen. 2006. Cerebral Toxoplasmosis: Pathogenesis and Host Resistance. In:
Toxoplasma gondii - a Model Apicomplexan; Perspectives and Methods. Kami Kim and L.M. Weiss,
Eds; Elsevier Press
Halonen, S.K., T. Woods, K. McInnerney, and L.M. Weiss. 2006. Microarray Analysis of the IFNγ
response genes in Astrocytes. J. Neuroimmunology 175:19-30.
Dzierszinski, F., M., M. Pepper, J.S. Stumhofer, D.F. LaRosa, E. H. Wilson, L.A. Turka, S.K.
Halonen, C.A., Hunter, and D.S. Roos. 2007. Presentation of Toxoplasma gondii antigens via
the endogenous major histocompatibility complex Class I pathway in non-professional and
professional antigen presenting cells. J. Immunol. 75 (11): 5200-9.
Halonen, S.K. and L.M. Weiss. 2008. Toxoplasma gondii IWOP10 2008: One Hundred Years and
Counting. Submitted to J. Eukaryotic Microbiology July 2008; invited submission
Melzer, T.A., A. Duffy, L.M. Weiss and S.K. Halonen. 2008. IGTP is necessary for Toxoplasma
Vacuolar Disruption and Induces Parasite Egression in IFNγ Stimulated Astrocytes;
Infection and Immunity 76(11):4883-4894.
Halonen, S.K. 2009. Role of Autophagy in the Host Defense against Toxoplasma gondii in
Astrocytes. Autophagy 5(8):268-269.
PHS 398/2590 (Rev. 09/04)
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Principal Investigator/Program Director (Last, First, Middle):
Halonen, S.K.
Halonen, S.K., T.C. Melzer, H.J. Cranston, and L.M. Weiss. 2010. Host Cell Preference of
Toxoplasma gondii in Murine Brain: A confocal study. Journal of Neuroparasitology; June 2010.
Zhang, Y.W., S.K. Halonen, Y.F. Ma, H.B. Tanowitz and L.M. Weiss. 2010. A Purification
Method for Enrichment of the Toxoplasma gondii Cyst Wall. J. Neuroparasitology.
Halonen, S.K. and L.M. Weiss. 2013. Toxoplasmosis; Handbook of Clinical Neurology, Volume
114 (3rd Series); Neuroparasitology and Tropical Neurology; pgs. Elsevier Science.
Y. Suzuki, O. Sa, E. Ochai, J. Mullins, R. Yolken, and S. Halonen. 2013. Cerebral
Toxoplasmosis: Pathogenesis, Host Resistance and Behavioral Consequences In: Toxoplasma
gondii - a Model Apicomplexan; Perspectives and Methods; p. 751 – 783; Second Edition. Kami
Kim and L.M. Weiss, Elsevier Press.
S.K. Halonen., Melzer, T.A. H.J. Cranston, J. Gress, K. McInnerney, A. Mazurie, and E. Dratz. 2014.
Differential gene expression in murine astrocytes infected with virulent (type I) vs. avirulent (type II)
strains of Toxoplasma gondii. 2014. Journal of Neuroinfectious Diseases 5(2).
S.K.Halonen. Modulation of Host Programmed Cell Death Pathways by the Intracellular
Protozoan Parasite, Toxoplasma gondii: implications for maintenance of chronic infection
and potential therapeutic applications; Manuscript accepted for publication in Book entitled “Cell
Death – Autophagy, Necrosis and Apoptosis”; anticipated publication date: May 2015
Tanaka, N., H.J. Cranston, W. Maaty, B. Bothner and S.K. Halonen. Proteomic Host Response to
Toxoplasma gondii strains of distinct virulence phenotypes. Manuscript in Preparation.
D. Research Support
Completed Research Grants:
IFNγ Inhibition of Toxoplasma gondii in Astrocytes
P.I.: Sandra K. Halonen
Agency: National Institute of Allergy & Infectious Disease
Type: R21 AI64057-01
Funding Period: 3/14/05 – 3/15/07
Major Goal: To investigate the mechanism of IFNγ inhibition of Toxoplasma gondii in astrocytes.
IFNγ Immune Effector Mechanisms in Cerebral Toxoplasmosis
P.I.: Sandra K. Halonen
Agency: National Institute of Allergy & Infectious Disease
Type: R21 AI64652-01
Funding Period: 3/14/05- 3/14/08
Major Goal: To investigate the mechanism of IGTP-dependent IFNγ induced inhibition of
Toxoplasma gondii in astrocyte
Toxoplasma gondii and Schizophrenia: Pilot Study
P.I.: Sandra K. Halonen
Agency: Stanley Medical Research Institute
Funding Period: 3/01/08 - 2/28/11
Major Goal: To investigate the possible role of infection with Toxoplasma gondii and Schizophrenia via
RT-PCR and immunohistochemistry using human brain tissues of schizophrenia positive patients.
Global Analysis of the Host Cell Response to Infection with Toxoplasma gondii in Astrocytes
P.I.: Edward Dratz; Project P.I.: S.K. Halonen
Agency: National Institute of Health
Type: COBRE (P-30)
Funding Period: 9/26/08 – 2/28/14
Major Goal: To define the IFNγ response to Toxoplasma gondii infection in astrocytes at the
transcriptional and proteomic levels.
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Principal Investigator/Program Director (Last, First, Middle):
Halonen, S.K.
Grants Funded:
Neural Stem Cells and 3D Culture Models
P.I.: Sandra K. Halonen
Agency: Gulf Manco Group Co.
Type: Fellowship Award
Funding Period: 09/01/14-05/15/18
Major Goal: Neural Stem Cells (NSCs) are partially differentiated pluripotent stem cells that have
the ability to generate all types of cells found in the central nervous system (CNS). NSCs derived
differentiated cells will be used to generate cerebral organoid cultures, 3-D brain-like masses,
created in vitro. The cerebral organoids created by these studies will be used to model Cerebral
Toxoplasmosis and other disease processes in the CNS.
Grants Submitted (pending):
Toxoplasma gondii and the Nervous System
P.I.: Louis M. Weiss; Consortium Investigator: S.K. Halonen
Agency: NIH-NIAID
Type: R01 (Submitted 06/05/14, not funded;
resubmission in consideration)
Funding Period: April 2016-March 2020
Major Goal: The effect of T. gondii infection will be examined using imaging
mass spectrometry to examine small molecule profiles surrounding T. gondii cysts in mouse
brains with latent infection. Ultrastructural and SeeDB approaches will be used to characterize the
distribution and formation of T. gondii tissue cysts.
Grants in Preparation:
Proteomic Analyses of Astrocyte Host Cell Responses to Toxoplasma gondii strains of Distinct
Virulence Phenotypes
P.I.: Sandra K. Halonen
Agency: NIH-NIAID
Type: R21 (Anticipated Submission Date: 06/16/15)
Funding Period: 12/1/15 -11/30/17
Major Goal: To identify astrocyte host cell proteins and host targets of parasites kinases
and phosphatases that are differentially regulated during parasite infection and
explore how these host cell changes are altered by the parasites of distinct virulent
phenotypes.
Model of Cerebral Toxoplasmosis in Human Neuronal Cells
P.I.: Sandra K. Halonen
Agency: NIH-NIMH
Type: R01 (Anticipated Submission Date: 10/05/15)
Funding Period: 4/1/16-3/30/20
Major Goal: Human neurons and astrocytes, derived from ihPSC’s will be used to model
Cerebral Toxoplasmosis to further our understanding of chronic Toxoplasma infection in the
immunocompetant host and its possible etiological role in psychiatric disorders such as
Schizophrenia. Transcriptional, proteomic and cellular assays incorporating optogenetic approaches,
will be used to assess functional impact of the parasite on neuronal functions.
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