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Transcript
Section 4.3 NLRD GMO details
Instructions
1.
Use the table below to enter the details of all GMOs being used in the project (See example table at the end for reference). Add more rows if
necessary.
2. Determine the GMO dealing classification
3. Upload completed table in Section 7 of the online form
4.3a
Common
name of
parent
organism
Scientific name of
parent organism
(must include genus
and species name if
applicable)
4.3b
4.3c
4.3e and f
4.3g
Vector(s) and
method of transfer
Exempt
Host/vector
system?
Identity and function
of gene(s) and
organism of origin
Organisms or
tissues to be used
with the GMO(s)
NLRD type
PC1 (a)-(c)
NLRD type
PC2
(a)-(m)
Example Table - Examples of responses
The “parent organism” means the organism(s) (or tissue derived from organisms) that you propose to genetically modify and “host” equates to “parent”.
4.1
4.2
4.3
4.5 and 4.6
4.7
Vector(s) and
method of transfer
Exempt
Host/vector
system?
Identity and function
of gene(s) and
organism of origin
Organisms or
tissues to be used
with the GMO(s)
Common
name of
parent
organism
Scientific name of
parent organism
Mouse
Mus musculus
Bacterial artificial
chromosomes
(BACs)
microinjected into
mouse embryos
(knockout IGFII)
no
IGFII from Macropus
eugenii (Tammar
Wallaby)
none
Thale cress
Arabidopsis thaliana
Non tumorigenic
disarmed Ti
plasmid via vacuum
infiltration.
no
GFP gene from
Aequorea victoria
none
Human cell
line
HER911
Transfection with
replication
deficient
adenovirus
no
EGFR from Mus
musculus
none
Bacteria
Enterophathogenic
Escherichia coli
Standard nonconjugative cloning
vector pUC,
pBluescript by
electroporation
no
E. coli DNA fragments
Mus musculus will
be injected with
modified bacteria
NLRD type
PC1 (a)-(c)
NLRD type
PC2
(a)-(m)
a
b
c
d
Yeast
Saccharomyces
cerevisiae
Standard yeast
expression vector
yes
CDNA library from
Homo sapiens (to
investigate cell cycle
regulators- potential
oncogenes)
none
Exempt