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Section 4.3 NLRD GMO details Instructions 1. Use the table below to enter the details of all GMOs being used in the project (See example table at the end for reference). Add more rows if necessary. 2. Determine the GMO dealing classification 3. Upload completed table in Section 7 of the online form 4.3a Common name of parent organism Scientific name of parent organism (must include genus and species name if applicable) 4.3b 4.3c 4.3e and f 4.3g Vector(s) and method of transfer Exempt Host/vector system? Identity and function of gene(s) and organism of origin Organisms or tissues to be used with the GMO(s) NLRD type PC1 (a)-(c) NLRD type PC2 (a)-(m) Example Table - Examples of responses The “parent organism” means the organism(s) (or tissue derived from organisms) that you propose to genetically modify and “host” equates to “parent”. 4.1 4.2 4.3 4.5 and 4.6 4.7 Vector(s) and method of transfer Exempt Host/vector system? Identity and function of gene(s) and organism of origin Organisms or tissues to be used with the GMO(s) Common name of parent organism Scientific name of parent organism Mouse Mus musculus Bacterial artificial chromosomes (BACs) microinjected into mouse embryos (knockout IGFII) no IGFII from Macropus eugenii (Tammar Wallaby) none Thale cress Arabidopsis thaliana Non tumorigenic disarmed Ti plasmid via vacuum infiltration. no GFP gene from Aequorea victoria none Human cell line HER911 Transfection with replication deficient adenovirus no EGFR from Mus musculus none Bacteria Enterophathogenic Escherichia coli Standard nonconjugative cloning vector pUC, pBluescript by electroporation no E. coli DNA fragments Mus musculus will be injected with modified bacteria NLRD type PC1 (a)-(c) NLRD type PC2 (a)-(m) a b c d Yeast Saccharomyces cerevisiae Standard yeast expression vector yes CDNA library from Homo sapiens (to investigate cell cycle regulators- potential oncogenes) none Exempt