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Download title: green synthesis of silver nanoparticles and its cancer cells
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ABSTRACT SUB SECTOR OF NANOTECHNOLOGY: HEALTHCARE NANO TITLE: GREEN SYNTHESIS OF SILVER NANOPARTICLES AND ITS CANCER CELLS INHIBITION STUDIES V R Keerthi1, S G Bhavya1 and K. N. Chidambara Murthy2 1Department of Biotechnology, M. S. Ramaiah Institute of Technology, Bengaluru- 560 054, India. 2Central Research Laboratory, M.S. Ramaiah Medical College and Teaching Hospital, Bengaluru- 560 054, India. Nanomedicine is one of the fast emerging fields of nanotechnology, which deals with the application of nanoscaled materials in the area of Medicine/Health science. In the proposed study the medicinal properties of Decalepis hamiltonii along with silver has been made use for green synthesis of silver nanoparticle and examined for its Cytotoxic effect on human colon cancer cell lines (Caco-2). Biologically synthesized nanoparticles are among the most widely used nanoproducts which are synthesized using eco-friendly nontoxic reducing agent. The present study deals with the synthesis of silver nanoparticles using aqueous root extract of Decalepis hamiltonii under optimised condition by varying process parameters like volume of plant extract, concentration of silver nitrate, temperature, ratio of plant extract to silver nitrate solution. Additionally, we have also measured proliferation inhibition efficiency of silver nanoparticles on colon cancer cell lines. The synthesis of nanoparticles was confirmed by UV- Visible spectrophotometer. Further, it was characterised by XRD, FTIR & SEM. The micrometric analysis by SEM demonstrated spherical shape of particles with size ranging from 50-70 nm. The cancer cell inhibition study was done using cytotoxicity (MTT) Assay and Cytomorphology. There was more than 50% cell death at 10µM concentration of Silver Nanoparticles after 24h incubation depicting the IC50 concentration of synthesized nanoparticles. The cytomorphology observation showed cytotoxicity caused due to membrane blebbing and induction of programmed cell death (Apoptosis).
