Download Cytotoxicity and Uptake of Nanoparticles in Cells

Cytotoxicity
and Uptake of
Nanoparticles in Cells
Courtney Rister
Faculty Advisors:
Virginia Ferguson
Conrad Stoldt
Department of Mechanical Engineering
University of Colorado, Boulder
Objective

How does nanoparticle presence affect
cellular function and behavior?


Little is known on how they interconnect
with cytoskeleton
Long term goals
Which nanoparticles are most efficient?
 What is the best way to deliver the
nanoparticles?

October 15, 2004
Introduction to Research
Presentation
2
Background

Uses of magnetic nanoparticles in bioengineering

Therapeutic



Hyperthermia
Drug Delivery
Diagnostic

Nuclear Magnetic
Resonance (NMR)
October 15, 2004
Introduction to Research
Presentation
3
Methodology



Perform cell cultures with Mammalian SaOS-2 cells
Obtain either Magnetite or Gadolinium nanoparticle
cells
Encapsulate nanoparticles in liposomes


Prevents formation of large aggregates and increases
biocompatibility
Introduce nanoparticles to SaOS-2 cells and record
effect
October 15, 2004
Introduction to Research
Presentation
4
Requirements


TEM (Transmission Electron Microscope)
BioServe equipment & supplies
Cells
 Culture media and facilities

October 15, 2004
Introduction to Research
Presentation
5
Timeline
Oct.
Nov.
Dec.
Jan.
Feb.
March
April
May
Literature
Review
Initial
Project
Design
Cell
Cultures
Finalize
Project
Plans
Finalize
Experiment
al Plans
Perform
Experiments
October 15, 2004
Introduction to Research
Presentation
6