Download CURRICULUM VITAE - GITAM Institute of Science

Satyanarayana Swamy Cheekatla, Ph.D
Assistant Professor
Department of Biotechnology
Institute of science
GITAM University
Visakhapatnam – 530 045
India
20-6/1-5/1
SBI North Street
Kakinada – 533 001
Andhra Pradesh, India.
e-mail: [email protected]
Mob: +91-9491941088
BACKGROUND SUMMARY
~4 years post-Ph.D experience in Infection Immunology with expertise in handling Mycobacterium
tuberculosis and other Infectious agents in Bio-safety Level 3 conditions; Strong background in
Autoimmune and Inflammatory Immunology.
PROFESSIONAL EXPERIENCES
1.
ASSISTANT PROFESSOR (September 2016 - Present)
Department of Biotechnology, GITAM University, Visakhapatnam, India
2.
POST-DOCTORAL FELLOW (Jan 2013 – August 2016)
Department of Pulmonary Immunology, UT Health Northeast, Tyler, Texas, USA
 Developed type 2 diabetic mouse model and involved in identifying cellular and molecular
mechanisms involved in the predisposition of diabetic mice to mycobacterium tuberculosis
infection
 Characterization of Innate memory like Natural Killer cells in BCG vaccine induced protective
immunity to M. tuberculosis
 Identification of vital role of IL-21 in sustaining antigen specific T cell responses during chronic
tuberculosis infection
 Mechanism of sustained prolonged pancreatic Islet Allograft survival during transplantation
3.
JUNIOR RESEARCH FELLOW (Nov 2005 – Feb 2007)
Department of Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences
(SGPGIMS), Lucknow, India
 Characterization of peripheral blood and synovial fluid T cell responses to citrullinated selfproteins in patients with Rheumatoid Arthritis
 Phenotypic and Functional characterization of peripheral blood and synovial fluid T cells in
Juvenile Idiopathic Arthritis (JIA)
 Urinary CXCL-10/IP-10 and MCP-1 as markers to assess activity of lupus nephritis
4.
RESEARCH INTERN (Jan 2005 - Nov 2005)
GRB Division, Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, India.
 Pharmacological screening of various medicinal plants for antiepileptic potential in different
animal models (Mouse & Drosophila Mutants)
EDUCATION
DOCTORAL
Feb 2012; PhD (Immunology), Department of Immunology, SGPGIMS,
Lucknow, India (under supervision of Prof. Ramnath Misra). The thesis
title is "Mechanisms of cytokine modulation in macrophages by L.
donovani (protozoan parasite) infection ".
Identified the signal transduction pathway responsible for the cytokine
modulation from Th1 to Th2 cytokine profile (THP-1 cell line & Human
Monocyte derived macrophages).
POSTGRADUATE
June 2004; M.Sc.(Biotechology), Andhra University, Visakhapatnam,
India. Dissertation title was ‘Comparative study of liver enzymes in
relation with serum protein profile in liver diseases’. To establish serum
biomarkers and diagnose different groups of Liver Hepatotoxicity
(Cirrhosis, Viral hepatits & Amoebic liver abscess).
GRADUATE
June 2002; B.Sc.(Biotechology), Andhra University, Visakhapatnam,
India. Major subjects: Biotechnology, Biochemistry & Chemistry
REWARDS
1.
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6.
International travel award from ICMR, New Delhi to attend AAI-2011, San Francisco on may 13th 17th, 2011.
International travel award from Department of Biotechnology, New Delhi to attend AAI-2011,
San Francisco, USA on may 13th -17th, 2011
Qualified joint CSIR-UGC National Eligibility Test (NET) in Life Sciences as Junior Research Fellow
(Council for Scientific and Industrial Research, Government of India) held on June 2006 and
recipient of JRF and SRF research fellowships during Ph.D
Lectureship – National Eligibility Test (NET) conducted by CSIR-UGC, India. qualified 5 successive
times (December 2003, 2004, 2005, 2006 & June 2005)
Research Intern – “CSIR Diamond Jubilee Research Interns Awards” Scheme for a period of 2
years.
Graduate Aptitude Test in Engineering (GATE)- 2005 in Life Sciences discipline with 90.86
Percentile (All India Rank-591), conducted by Indian Institute of Technology (IIT), Madras, India.
PUBLICATIONS
1.
Tripathi D, Venkatasubramanian S, Cheekatla SS, Paidipally P, Welch E, Tvinnereim AR,
Vankayalapati R. A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in
type 1 diabetic mice. Nat Commun. 2016 Dec 16;7:13896. doi: 10.1038/ncomms13896.
2.
Venkatasubramanian S, Cheekatla S, Paidipally P, Tripathi D, Welch E, Tvinnereim AR, Nurieva R,
Vankayalapati R. IL-21-dependent expansion of memory-like NK cells enhances protective
immune responses against Mycobacterium tuberculosis. Mucosal Immunol. 2016 Dec 7. doi:
10.1038/mi.2016.105. [Epub ahead of print]
3.
Cheekatla SS, Tripathi D, Venkatasubramanian S, Nathella PK, Paidipally P, Ishibashi M, Welch E,
Tvinnereim AR, Ikebe M, Valluri VL, Babu S, Kornfeld H, Vankayalapati R. NK-CD11c+ cell crosstalk
in diabetes enhances IL-6-mediated inflammation during Mycobacterium tuberculosis infection.
PLoS Pathog. 12(10):e1005972 (2016)
4.
Venkatasubramanian S, Tripathi D, Tucker T, Paidipally P, Cheekatla S, Welch E, Raghunath A,
Jeffers A, Tvinnereim AR, Schechter ME, Andrade BB, Mackman N, Idell S, Vankayalapati R. Tissue
factor expression by myeloid cells contributes to protective immune response against
Mycobacterium tuberculosis infection. Eur J Immunol. 46(2):464-79 (2016).
5.
Venkatasubramanian S, Dhiman R, Paidipally P, Cheekatla SS, Tripathi D, Welch E, Tvinnereim AR,
Jones B, Theodorescu D, Barnes PF, Vankayalapati R. A Rho GDP Dissociation inhibitor produced
by apoptotic T-cells inhibits growth of Mycobacterium tuberculosis. PLoS Pathog. 11(2):e1004617
(2015).
6.
Reddy VV, Myles A, Cheekatla SS, Singh S, Aggarwal A. Soluble CD25 in serum: a potential marker
for subclinical macrophage activation syndrome in patients with active systemic onset juvenile
idiopathic arthritis. Int J Rheum Dis. 17(3):261-7 (2014).
7.
Abujam B, Cheekatla S & Aggarwal A; Urinary CXCL-10/IP-10 and MCP-1 as markers to assess
activity of lupus nephritis. Lupus. 22(6):614-23 (2013).
8.
Cheekatla SS, Aggarwal A, Naik S; mTOR signaling pathway regulates the IL-12/IL-10 axis in
Leishmania donovani infection. Med Microbiol Immunol. 201(1):37-46 (2012).
9.
Verma A, Prasad KN, Cheekatla SS, Nyati KK, Paliwal VK, Gupta RK; Immune response in
symptomatic and asymptomatic Neurocysticercosis. Med Microbiol Immunol. 200(4):255-61
(2011)
10.
Cheekatla SS and Vikas Agarwal; Autoimmunity
Indian Journal of Rheumatology (2011) 6, 26–27 (Review Article)
PAPERS PRESENTED IN CONFERENCES
1. Cheekatla S, Tripathi D, Venkatasubramanian S, Nathella P, Paidipally P, Ishibashi M, Welch E,
Tvinnereim AT, Ikebe M, Subash Babu, Kornfeld H and Vankayalapati R. NK-DC crosstalk in diabetes
enhances IL-6-mediated inflammation during tuberculosis infection. Tuberculosis Co-Morbidities
and Immunopathogenesis (Keystone Symposia, March 2016), Colorado, USA. [Short talk]
2. Satyanarayana Swamy Cheekatla, Deepak Tripathi, Sambasivan Venkatasubramanian, Padmaja
Paidipally, Elwyn Welch, Amy R. Tvinnereim, Hardy Kornfeld and Ramakrishna Vankayalapati. IL-6
regulates pro- and anti-inflammatory cytokine production and mortality of Mycobacterium
tuberculosis infected type 2 diabetic mice. Immunology 2015, New Orleans, USA.
3. Satyanarayana Swamy Cheekatla, Sambasivan Venkatasubramanian, Deepak Tripathi, Padmaja
Paidipally, Elwyn Welch, Amy R. Tvinnereim, and Ramakrishna Vankayalapati IL-21 is essential for
the optimal control of Mycobacterium tuberculosis infection. Immunology 2015, New Orleans, USA.
4. Deepak Tripathi, Sambasivan Venkatasubramanian, Satyanarayana S. Cheekatla, Padmaja
Paidipally, Elwyn Welch, Amy R. Tvinnereim, R. Vankayalapati. Liver NK1.1 cells and IL-22 promote
pancreatic islets allograft survival in Type 1 diabetic mice. Immunology 2015, New Orleans, USA.
5. Deepak Tripathi, Sambasivan Venkatasubramanian, Satyanarayana S. Cheekatla, Padmaja
Paidipally, Elwyn Welch, Amy R. Tvinnereim, R. Vankayalapati. CD4+CD25+Foxp3+ cells from JNK-/mice prolong pancreatic allograft survival in type 1 diabetic mice. Immunology 2015, New Orleans,
USA.
6. Venkatasubramanian S, Dhiman R, Paidipally P, Cheekatla SS, Tripathi D, Welch E, Tvinnereim AR,
Jones B, Theodorescu D, Barnes PF, Vankayalapati R. A Rho GDP Dissociation inhibitor produced by
apoptotic T-cells inhibits growth of Mycobacterium tuberculosis. Immunology 2015, New Orleans,
USA.
7. Sambasivan Venkatasubramanian, Padmaja Paidipally, Satyanarayana Cheekatla, Elwyn Welch,
Anjana Raghunath, Amy R. Tvinnereim, Amanda B. Barnes, Shirley Li, Abhinav Vankayalapati, Deepak
Tripathi, Roza Nurieva, Peter F. Barnes , Vijayalakshmi Valluri and Ramakrishna Vankayalapati. IL-21
dependent expansion of memory-like NK cells enhances protective immune responses against
Mycobacterium tuberculosis. NK2015, Quebec, Canada.
8. Abujam, Bonnie, Swamy, Satyanarayana, Aggarwal, Amita. Urinary and serum MCP-1 and IP-10 are
good markers to assess lupus activity. Asia Pacific League of Associations for Rheumatology, Amman,
Jordan, September 10-14, 2012
9. Satyanarayana Swamy Cheekatla, Amita Aggarwal, Sitanaik and Ramnath Misra. Leishmania
donovani modulates multiple immune genes in THP-1derived macrophages: A gene expression
profile analysis. Federation of Immunological Societies of Asia Oceania, New Delhi, India, March 1417, 2012.
10. Abujam, Bonnie, Swamy, Satyanarayana, Aggarwal, Amita. Urinary and Serum Monocyte
Chemotactic Protein-1 and Interferon Gamma-Induced Protein-10 are good markers to assess Lupus
activity. American College of Rheumatology/Annual Scientific Meeting, Chicago, Illinois November 49, 2011.
11. Abujam, Bonnie, Swamy, Satyanarayana, Aggarwal, Amita. Urinary and serum MCP-1 and IP-10 are
good markers to assess lupus activity. National conference on Pediatric Rheumatology, Lucknow,
India September 2011.
12. B Abujam, S Swamy, A Aggarwal. Urinary MCP-1 and IP-10 as potential biomarkers for lupus
nephritis. Australian Rheumatology Association/Annual Scientific Meeting, Brisbane, Queensland,
May 14-17, 2011
13. Satyanarayana Swamy Cheekatla, Amita Aggarwal & Sitanaik; mTOR signaling pathway regulates
the IL-12/IL-10 axis in Leishmania donovani infection. IMMUNOLOGY 2011, Sanfrancisco, USA.
RELEVANT EXPERTISE
IMMUNOLOGY
IN VIVO
MOLECULAR BIOLOGY
BIOCHEMISTRY
CELL BIOLOGY
Primary cell isolation (including B cells, T cells, Macrophages, DCs) using
magnetic beads (MACS) and other methods, radioactive and nonradioactive-based proliferation assays, Immunophenotyping and
detection of activation markers by flow cytometry, Detecting cytokine
profiles in T-lymphoctyes subsets by intracellular flow cytometry,
ELISPOT, ELISA, and multiplexing techniques, T-cell epitope mapping,
Cytotoxicity assays, Studies on Apoptosis, signal transduction studies, in
vitro T-helper cell differentiation studies, multiplex analyte assays.
Handling of human clinical samples, eg. blood, synovial fluid, and
tissues.
Organ and primary cell isolation from Mice, Efficacy Models (Vaccine
evaluation studies, Safety, Immunogenicity via multiple immunization
routes, Dose-ranging and correlates of protection Challenge dose,
preparation and optimization via parenteral and respiratory routes,
including aerosol delivery,BSL-2 and BSL-3 containment).Determination
of bacterial loads & infectivity assays. Experience in inducing diabetes &
epilepsy in mouse model.
Electrophoretic mobility shift assay (EMSA), Supershift, DNA & RNA
isolation, Polymerase Chain Reaction (PCR), Agarose electrophoresis
and PAGE, Gel Documentation, Densitometry, Western Blotting, 2D
Electrophoresis and Real-Time PCR. Isolation of Plasmids from
Microbes, Competent cells preparation and transformation. Genecloning.
Experience in siRNA functional studies, protein or Enzyme purification,
Fractionation, protein quantification methods.
Animal cell culture, Generation of synovial fibroblasts from synovial
fluid; Generation of antigen specific T-cell Lines; B-cell immortalization
by EBV; Maintenance of Cell lines: THP-1, RAW 264.7, Jurkat, MDCKII,
K562, B95.8, JAR & JEG-3.
COMPUTER & BIOINFORMATIC PROFICIENCY
MS-office, SPSS, GraphPad Prizm, Multiparametric flow cytometry, sequence analysis, primer design,
phylogeny, alignments and other data analysis and documentation software.
TRAINING
1.
April 2013 “3rd Texas Tuberculosis Research Symposium” at The University of Texas Health
Science Center, San Antonio, USA.
2.
January 2008, International training in global infectious diseases (GID), 3rd Workshop of the
Seattle-India Joint Research Training Program “Research Training on Intracellular Pathogens” at
Indian School of Business, Hyderabad, India
3.
December 2007, “8th Indo-US workshop on Flow-cytometry” (Clinical application), at Department
of Immunology, SGPGIMS, Lucknow, India.
4.
Presented seminars, journal clubs and attended academic sessions of Drug discovery, Immunology
and Molecular biology in the respective organizations
5.
Successfully completed foundation and compliance courses at SGPGIMS and UTHSCT (including
Research Methodology, Statistics, Research Compliance: Conflicts of Interest and Scientific
Misconduct, Lab Safety etc.)
PERSONAL INFORMATION
DATE OF BIRTH
CITIZENSHIP
GENDER
MARITAL STATUS
LANGUAGES
13th Aug 1981
Indian
Male
Married
English, Telugu & Hindi
REFERENCES
1. Ramakrishna Vankayalapati, Ph.D
Professor & Chair
Department of Pulmonary Immunology
The University of Texas Health Center
11937 US Hwy 271
Tyler, TX 75708
Office: +1(903) 877-5190
Lab: +1 (903) 877-7686
Fax: +1(903) 877-7989
E-mail: [email protected]
2. Ramnath Misra (M.D.)
3. Amita Aggarwal (D.M.)
4. Vikas Aggarwal (D.M.)
Professor
Department of Immunology,
Sanjay Gandhi Postgraduate Institute of Medical
Sciences, Raebareilly Road, Lucknow 226014,
India.
Phone: +91-9335265156
(O) +91-522-2494296;
Fax: +91-522-2668017
E-mail: [email protected]
Dean & Professor
Department of Immunology,
Sanjay Gandhi Postgraduate Institute of Medical
Sciences, Raebareilly Road, Lucknow 226014,
India
Phone: +91-8004904387
E-mail: [email protected]
Professor
Department of Immunology,
Sanjay Gandhi Postgraduate Institute of Medical
Sciences, Raebareilly Road, Lucknow 226014,
India.
Phone:+91-522-2494318
FAX:+91-522-2668017
E-mail: [email protected]