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Name: Biology 178-001 Spring 2006 Principles of Biology: Practice Exam 2 Do this exam AFTER studying and under exam conditions. Give yourself approximately ONE HOUR to do the exam. Review session is Weds, Feb 22 at 1 PM in Science 106. MULTIPLE CHOICE: 1. Water molecules cross the plasma membrane by: (A) active transport. (B) endoctytosis. (C) exocytosis. (D) osmosis. (E) B and C. 2. The removal of waste products from a cell is an example of: (A) osmosis. (B) endocytosis. (C) exocytosis. (D) receptor mediated endocytosis. (E) lysis. 3. Which of the following organisms have centrioles? (A) Animals (B) Plants (C) Bacteria (D) Viruses (E) All of the above 4. The endosymbiotic theory states that: (A) mitochondra and chloroplasts were once free-living cells, (B) mitochondria and chloroplasts can exist independently of the eukaryotic cell. (C) lysosomes were once prokaryotes. (D) all organelles have 2 membranes. (E) all of the above. 5. The ___________ can be described as “cell freeways”: (A) centrioles (B) cytoskeleton (C) endomembrane system (D) cilia (E) B and D 6. ___________ are responsible for the formation of a cleavage furrow in mitosis and meiosis. (A) Microtubules (B) Actin filaments (C) Intermediate filaments (D) Spindle fibers (E) Tubulin subunits 1 7. Which of the following is evidence for the Endosymbiotic Theory? (A) Centrioles have 2 membranes. (B) Mitochondria and chloroplasts have their own circular DNA. (C) Lysosomes, mitochondria, and chloroplasts are about the size of a bacterium. (D) B and C. (E) All of the above. 8. Cells crawl by pushing __________ into cytoplasmic extensions. (A) actin (B) tubulin (C) DNA (D) centrioles (E) cilia 9. Plant cells can grow by: (A) increasing the number of chloroplasts. (B) absorption of water into their vacuoles. (C) losing water from their vacuoles. (D) thickening of the plasma membrane. (E) diffusion. 10. Grana is/are: (A) the liquid that bathes the interior of the mitochondrion. (B) found in the chloroplasts. (C) stacks of thylakoids. (D) an aging cell. (E) B and C. 11. Which of the following types of transport require an input of energy? (A) Selective diffusion (B) Receptor mediated endocytosis (C) Osmosis (D) Active transport (E) B and D 12. The function of this organelle is to digest worn-out parts of cells. The organelle is the: (A) Golgi complex. (B) chloroplast. (C) mitochondrion. (D) lysosome. (E) endoplasmic reticulum. 13. The Golgi complex functions in: (A) protein synthesis. (B) heredity. (C) modification of ER products and packing, sorting, and shipping proteins from the cell. (D) detoxification. (E) energy production. 14. The endomembrane system includes: (A) ER, Golgi, and mitochondria. (B) ER, Golgi, and lysosomes. (C) nuclear envelope, plasma membrane, and microtubules. (D) centrioles and basal bodies. (E) none of the above. 2 15. You have genetically engineered tubulin subunits so that they are expressing green fluorescent protein (GFP). You introduce these to a mixture containing non-genetically engineered microtubules and ATP. You then place the mixture on a microscope slide that has non-genetically engineered kinesin stuck to it by the end that would normally bind the cargo molecules. Finally, you observe the slide using a fluorescence microscope. What would you see? (A) No movement (B) The microtubules advancing across the slide with the large fluorescent end in front (C) The microtubules advancing across the slide with the small fluorescent end in front (D) The kinesin moving across the slide (E) Nothing – there would be no fluorescent materials to view in this experiment 16. If you repeated the above experiment with dynein, what cytoskeletal element would you need to include? (A) Microtubules (B) Intermediate filaments (C) Actin filaments (D) Keratin (E) None of the above 17. Chromosome structure is partly maintained by: (A) positive charge on the DNA. (B) no charge on the DNA. (C) positive charge on the histones. (D) no charge on the histones. (F) negative charge on the histones. 18. Which of the following does not involve the plasma membrane? (A) Receptor mediated endocytosis (B) Active transport (C) Secretion (D) Pinocytosis (E) None of the above 19. Which of the following uses clathrin? (A) The sodium-potassium pump (B) The sodium-glucose symport (C) Osmosis (D) Receptor mediated endocytosis (E) ATP hydrolysis 20. Which of the following involves transport of substances up a concentration gradient? (A) Sodium-potassium pump (B) Osmosis (C) Facilitated diffusion (D) Secretion (E) All of the above 21. Protein signal sequences: (A) are hydrophilic. (B) are incorporated into the final protein structure. (C) only occur on proteins that are to remain within the cell. (D) signal the ribosome to dock onto the Golgi apparatus. (E) none of the above. 3 22. The following statement describes ________. This filamentous structure is composed of two intertwined chains of a globular protein and is approximately 7 nm diameter. (A) actin filaments (B) keratin (C) intermediate filaments (D) microtubules (E) none of the above 23. Which of the following is not a component of the extracellular matrix? (A) Cytoplasm (B) Elastin (C) Proteoglycan (D) Collagen (E) Glycoprotein 24. What problem(s) might a person with hypercholesterolemia have? (A) Cancer (B) Defective LDL receptors (C) Cell membrane fluidity problems (D) Atherosclerosis (E) B, C, and D 25. The resting potential of the cell: (A) is a result of DNA replication. (B) is +70 mV. (C) is maintained by the sodium-potassium pump. (D) allows sodium to rapidly diffuse out of the cell. (E) is also known as an action potential. 26. Nuclear pores: (A) allow DNA to pass from the nucleus to the cytoplasm. (B) allow RNA to pass from the nucleus to the cytoplasm. (C) allow ribosomal subunits to pass from the nucleus to the cytoplasm. (D) B and C. (E) all of the above. 27. Ribosomes are made of: (A) DNA and protein. (B) protein only. (C) DNA and RNA. (D) carbohydrate. (E) RNA and protein. (Multiple choice = 27 points) The rest of the answers go on the exam. 28. Briefly describe the structure and function of the following below (you may include diagrams if you wish): (a) Nucleus (i) Structure – (ii) Function – 4 (b) Nucleolus (i) Sructure – (ii) Function – (c) Rough endoplasmic reticulum (i) Structure – (ii) Function – (d) Lysosomes (i) Structure – (ii) Function – (e) Nuclear envelope (i) Structure – (ii) Function – (f) Actin filaments (i) Sructure – (ii) Function – (g) Microtubules (i) Structure – (ii) Function – (h) Golgi complex (i) Structure – (ii) Function – (j) Smooth endoplasmic reticulum (i) Structure – (ii) Function – 5 (k) Mitochondrion (i) Sructure – (ii) Function – (l) Chloroplast (i) Structure – (ii) Function – (m) Centriole (i) Structure – (ii) Function – (n) Plant cell vacuole (i) Structure – (ii) Function – (o) Plant cell wall (i) Sructure – (ii) Function – (p) Intermediate filaments (i) Sructure – (ii) Function – (30 points) 6 29. Read the following and write up in the format of the scientific method (include a question, hypotheses, predictions, controlled experiment, and possible conclusions (based on what you might observe)). The drawing below shows an osmometer, which is an instrument used to measure osmotic pressure. Pure water is separated from a sugar solution by a membrane that is permeable to water, but not the sugar molecules. Osmotic pressure can be measured by the movement of the piston. (6 points) 7