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COST OF INVESTMENTS We do not ask any investments. MATERIAL COST Operating cost Materials Materials comprise of expenses for bacteria and Arabidopsis cultivation media, selection antibiotics, polymerases for cloning and plant genotyping, restriction endonucleases for conventional cloning, BP and LR clonases for Gateway cloning, new destination vectors for the Gateway system, isolation kits for DNA and RNA, DNA purification kits, oligonucleotide synthesis, microtubule and actin destabilizing drugs. Further operating cost will cover laboratory plastics and consumption material for microscopy. We also intend to order several insertional Arabidopsis mutants (SALK Institute). Overhead Overhead represents 15% of total material cost. Social and medical insurances Social and medical insurances represent 37% of total labor cost. Services In the first year, larger amount of total material cost will be devoted to services because we intend to analyze transcriptome of the exo70A1 mutant. This service is commercially available in NASC's International Affymetrix Service. Further, services comprise of payment for sequencing of prepared constructs, printing of a poster for a conference. Traveling cost Traveling cost covers registration fee and (partially) traveling expenses for a scientific conference where our preliminary results will be presented. It is not possible to specify a particular conference yet, however, we consider the International Workshop on Plant Membrane Biology for the first year. LABOR COST The principle investigator does not ask salary, due to a participation in the LC 06034 project. Labor cost covers bonus for the principle investigator, salary (0.25 work load) for the coinvestigator and other personal expenses for technical stuff (approx. 80 hours per year) for occasional cultivation of a large amount of experimental plants.