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Download Week 5 Assignment 1. Reverse Phase Arrays (RPA) involve printing
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Transcript
Week 5 Assignment 1. Reverse Phase Arrays (RPA) involve printing of ________? 0.5 points Tissue sections Cell lysates Purified proteins Peptide segments 2. A protein array with purified proteins printed on it belongs to which of the following categories of arrays when probed with serum samples containing autoantibodies? 0.5 points Forward Phase arrays Reverse phase arrays 3. Which of the following is not true for direct labeling? 0.5 points Only single antibodies are required High reproducibility High sensitivity in detecting low abundance proteins Multi-sample assay 4. Which of the following is a conventional labeling technique in protein microarrays? 0.5 points Chromogenic detection techniques Chemiluminiscence based detection techniques Fluorescence based detection techniques Radioactivity based detection techniques All of the above 5. Raman dye labelled proteins/antibodies can be used in which of the following novel detection techniques? 0.5 points SWNTs Quantum dots Raman dye labeled nanoparticles a and c b and c 6. Which of the following novel detection techniques relies on DNA hybridization? 0.5 points SWNTs Quantum dots Nano particle based Bio-barcodes Raman dye labeled nanoparticles 7. Recombinational cloning through gateway cloning DOES NOT require which of the following components? 0.5 points Expression clone Donor Vector Expression Vector Co-dominant clone 8. Once a master clone is sequence verified, is it necessary to re-sequence the recombinant expression clone generated through gateway-cloning? 0.5 points Yes, is it necessary. These is a high probability of sequence could be modified during recombination process. No, it is not necessary. There is a high probability that the sequence would continue to remain unchanged. 9. An ideal cell free expression for a bacterial protein is? 0.5 points Rabbit reticulocyte lysate E.coli cell lysate Human Hela cell lysate None of the above 10. To have a cell free expression based array, one must print a chip with ________? peptides DNA proteins PNA 0.5 points
