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Chapter 13 Meiosis and Sexual Life Cycles PowerPoint® Lecture Presentations for Biology Eighth Edition Neil Campbell and Jane Reece Lectures by Chris Romero, updated by Erin Barley with contributions from Joan Sharp Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Overview: Variations on a Theme • Living organisms are distinguished by their ability to reproduce their own kind • Genetics is the scientific study of heredity and variation • Heredity is the transmission of traits from one generation to the next • Variation is demonstrated by the differences in appearance that offspring show from parents and siblings Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Concept 13.1: Offspring acquire genes from parents by inheriting chromosomes • In a literal sense, children do not inherit particular physical traits from their parents • It is genes that are actually inherited Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Inheritance of Genes • Genes are the units of heredity, and are made up of segments of DNA • Genes are passed to the next generation through reproductive cells called gametes (sperm and eggs) • Each gene has a specific location called a locus on a certain chromosome • Most DNA is packaged into chromosomes • One set of chromosomes is inherited from each parent Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Comparison of Asexual and Sexual Reproduction • In asexual reproduction, one parent produces genetically identical offspring by mitosis • A clone is a group of genetically identical individuals from the same parent • In sexual reproduction, two parents give rise to offspring that have unique combinations of genes inherited from the two parents Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Concept 13.2: Fertilization and meiosis alternate in sexual life cycles • A life cycle is the generation-to-generation sequence of stages in the reproductive history of an organism Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Sets of Chromosomes in Human Cells • Human somatic cells (any cell other than a gamete) have 23 pairs of chromosomes • A karyotype is an ordered display of the pairs of chromosomes from a cell • The two chromosomes in each pair are called homologous chromosomes, or homologs • Chromosomes in a homologous pair are the same length and carry genes controlling the same inherited characters Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 13-3b TECHNIQUE 5 µm Pair of homologous replicated chromosomes Centromere Sister chromatids Metaphase chromosome • The sex chromosomes are called X and Y • Human females have a homologous pair of X chromosomes (XX) • Human males have one X and one Y chromosome • The 22 pairs of chromosomes that do not determine sex are called autosomes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Each pair of homologous chromosomes includes one chromosome from each parent • The 46 chromosomes in a human somatic cell are two sets of 23: one from the mother and one from the father • A diploid cell (2n) has two sets of chromosomes • For humans, the diploid number is 46 (2n = 46) Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • A gamete (sperm or egg) contains a single set of chromosomes, and is haploid (n) • For humans, the haploid number is 23 (n = 23) • Each set of 23 consists of 22 autosomes and a single sex chromosome • In an unfertilized egg (ovum), the sex chromosome is X • In a sperm cell, the sex chromosome may be either X or Y Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Behavior of Chromosome Sets in the Human Life Cycle • Fertilization is the union of gametes (the sperm and the egg) • The fertilized egg is called a zygote and has one set of chromosomes from each parent • The zygote produces somatic cells by mitosis and develops into an adult Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • At sexual maturity, the ovaries and testes produce haploid gametes • Gametes are the only types of human cells produced by meiosis, rather than mitosis • Meiosis results in one set of chromosomes in each gamete • Fertilization and meiosis alternate in sexual life cycles to maintain chromosome number Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 13-5 Key Haploid gametes (n = 23) Haploid (n) Egg (n) Diploid (2n) Sperm (n) MEIOSIS Ovary FERTILIZATION Testis Diploid zygote (2n = 46) Mitosis and development Multicellular diploid adults (2n = 46) The Variety of Sexual Life Cycles • The alternation of meiosis and fertilization is common to all organisms that reproduce sexually • The three main types of sexual life cycles differ in the timing of meiosis and fertilization Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • In animals, meiosis produces gametes, which undergo no further cell division before fertilization • Gametes are the only haploid cells in animals • Gametes fuse to form a diploid zygote that divides by mitosis to develop into a multicellular organism Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 13-6a Key Haploid (n) Diploid (2n) n Gametes n n MEIOSIS 2n Diploid multicellular organism (a) Animals FERTILIZATION Zygote 2n Mitosis Fig. 13-6 Key Haploid (n) n Gametes n Mitosis n n MEIOSIS FERTILIZATION Diploid multicellular organism (a) Animals Zygote 2n Mitosis Mitosis n Spores Mitosis Mitosis n n n n MEIOSIS 2n Haploid unicellular or multicellular organism Haploid multicellular organism (gametophyte) Diploid (2n) n Gametes n n Gametes FERTILIZATION MEIOSIS 2n Diploid multicellular organism (sporophyte) n 2n Mitosis (b) Plants and some algae Zygote FERTILIZATION 2n Zygote (c) Most fungi and some protists • Plants and some algae exhibit an alternation of generations • This life cycle includes both a diploid and haploid multicellular stage • The diploid organism, called the sporophyte, makes haploid spores by meiosis Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Each spore grows by mitosis into a haploid organism called a gametophyte • A gametophyte makes haploid gametes by mitosis • Fertilization of gametes results in a diploid sporophyte Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 13-6b Key Haploid (n) Diploid (2n) Mitosis n Haploid multicellular organism (gametophyte) Mitosis n n n n Spores MEIOSIS Gametes FERTILIZATION 2n Diploid multicellular organism (sporophyte) 2n Mitosis (b) Plants and some algae Zygote • In most fungi and some protists, the only diploid stage is the single-celled zygote; there is no multicellular diploid stage • The zygote produces haploid cells by meiosis • Each haploid cell grows by mitosis into a haploid multicellular organism • The haploid adult produces gametes by mitosis Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 13-6c Key Haploid (n) Haploid unicellular or multicellular organism Diploid (2n) Mitosis Mitosis n n n n Gametes MEIOSIS FERTILIZATION 2n Zygote (c) Most fungi and some protists n • Depending on the type of life cycle, either haploid or diploid cells can divide by mitosis • However, only diploid cells can undergo meiosis • In all three life cycles, the halving and doubling of chromosomes contributes to genetic variation in offspring Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Concept 13.3: Meiosis reduces the number of chromosome sets from diploid to haploid • Like mitosis, meiosis is preceded by the replication of chromosomes • Meiosis takes place in two sets of cell divisions, called meiosis I and meiosis II • The two cell divisions result in four daughter cells, rather than the two daughter cells in mitosis • Each daughter cell has only half as many chromosomes as the parent cell Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings The Stages of Meiosis • In the first cell division (meiosis I), homologous chromosomes separate • Meiosis I results in two haploid daughter cells with replicated chromosomes; it is called the reductional division • In the second cell division (meiosis II), sister chromatids separate • Meiosis II results in four haploid daughter cells with unreplicated chromosomes; it is called the equational division Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 13-7-3 Interphase Homologous pair of chromosomes in diploid parent cell Chromosomes replicate Homologous pair of replicated chromosomes Sister chromatids Diploid cell with replicated chromosomes Meiosis I 1 Homologous chromosomes separate Haploid cells with replicated chromosomes Meiosis II 2 Sister chromatids separate Haploid cells with unreplicated chromosomes • At the end of meiosis, there are four daughter cells, each with a haploid set of unreplicated chromosomes • Each daughter cell is genetically distinct from the others and from the parent cell Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings A Comparison of Mitosis and Meiosis • Mitosis conserves the number of chromosome sets, producing cells that are genetically identical to the parent cell • Meiosis reduces the number of chromosomes sets from two (diploid) to one (haploid), producing cells that differ genetically from each other and from the parent cell • The mechanism for separating sister chromatids is virtually identical in meiosis II and mitosis Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 13-9 MITOSIS MEIOSIS Parent cell Chromosome replication Prophase Chiasma Chromosome replication Prophase I Homologous chromosome pair 2n = 6 Replicated chromosome MEIOSIS I Metaphase Metaphase I Anaphase Telophase Anaphase I Telophase I Haploid n=3 Daughter cells of meiosis I 2n MEIOSIS II 2n Daughter cells of mitosis n n n n Daughter cells of meiosis II SUMMARY Property Mitosis Meiosis DNA replication Occurs during interphase before mitosis begins Occurs during interphase before meiosis I begins Number of divisions One, including prophase, metaphase, anahase, and telophase Two, each including prophase, metaphase, anaphase, and telophase Synapsis of homologous chromosomes Does not occur Occurs during prophase I along with crossing over between nonsister chromatids; resulting chiasmata hold pairs together due to sister chromatid cohesion Number of daughter cells and genetic composition Two, each diploid (2n) and genetically identical to the parent cell Four, each haploid (n), containing half as many chromosomes as the parent cell; genetically different from the parent cell and from each other Role in the animal body Enables multicellular adult to arise from zygote; produces cells for growth, repair, and, in some species, asexual reproduction Produces gametes; reduces number of chromosomes by half and introduces genetic variability amoung the gametes • Three events are unique to meiosis, and all three occur in meiosis l: – Synapsis and crossing over in prophase I: Homologous chromosomes physically connect and exchange genetic information – At the metaphase plate, there are paired homologous chromosomes (tetrads), instead of individual replicated chromosomes – At anaphase I, it is homologous chromosomes, instead of sister chromatids, that separate Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Concept 13.4: Genetic variation produced in sexual life cycles contributes to evolution • Mutations (changes in an organism’s DNA) are the original source of genetic diversity • Mutations create different versions of genes called alleles • Reshuffling of alleles during sexual reproduction produces genetic variation Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Origins of Genetic Variation Among Offspring • The behavior of chromosomes during meiosis and fertilization is responsible for most of the variation that arises in each generation • Three mechanisms contribute to genetic variation: – Independent assortment of chromosomes – Crossing over – Random fertilization Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Independent Assortment of Chromosomes • Homologous pairs of chromosomes orient randomly at metaphase I of meiosis • In independent assortment, each pair of chromosomes sorts maternal and paternal homologues into daughter cells independently of the other pairs Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • The number of combinations possible when chromosomes assort independently into gametes is 2n, where n is the haploid number • For humans (n = 23), there are more than 8 million (223) possible combinations of chromosomes • We will do an independent assortment activity in class with crayons and a circle paper to demonstrate this, but n will only equal 2 or 3. Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 13-11-3 Possibility 2 Possibility 1 Two equally probable arrangements of chromosomes at metaphase I Metaphase II Daughter cells Combination 1 Combination 2 Combination 3 Combination 4 Crossing Over • Crossing over produces recombinant chromosomes, which combine genes inherited from each parent • Crossing over begins very early in prophase I, as homologous chromosomes pair up gene by gene Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • In crossing over, homologous portions of two nonsister chromatids trade places • Crossing over contributes to genetic variation by combining DNA from two parents into a single chromosome Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 13-12-5 Prophase I of meiosis Pair of homologs Nonsister chromatids held together during synapsis Chiasma Centromere TEM Anaphase I Anaphase II Daughter cells Recombinant chromosomes Random Fertilization • In some species mates are chosen at random, or there may be more than one mate at a time so who’s the daddy could be a guess. • Even when mates are known, which sperm gets to the egg first is completely random. There are millions to choose from and each is genetically different. • Each time fertilization occurs there will a different combination of genes, thus producing endless variation within every species. Chapter 14 Mendel and the Gene Idea PowerPoint® Lecture Presentations for Biology Eighth Edition Neil Campbell and Jane Reece Lectures by Chris Romero, updated by Erin Barley with contributions from Joan Sharp Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Concept 14.1: Mendel used the scientific approach to identify two laws of inheritance • Mendel discovered the basic principles of heredity by breeding garden peas in carefully planned experiments Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Mendel’s Experimental, Quantitative Approach • Advantages of pea plants for genetic study: – There are many varieties with distinct heritable features, or characters (such as flower color); character variants (such as purple or white flowers) are called traits – Mating of plants can be controlled – Each pea plant has sperm-producing organs (stamens) and egg-producing organs (carpels) – Cross-pollination (fertilization between different plants) can be achieved by dusting one plant with pollen from another Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-2 TECHNIQUE 1 2 Parental generation (P) Stamens Carpel 3 4 RESULTS First filial generation offspring (F1) 5 • Mendel chose to track only those characters that varied in an either-or manner • He also used varieties that were true-breeding (plants that produce offspring of the same variety when they self-pollinate) Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • In a typical experiment, Mendel mated two contrasting, true-breeding varieties, a process called hybridization • The true-breeding parents are the P generation • The hybrid offspring of the P generation are called the F1 generation • When F1 individuals self-pollinate, the F2 generation is produced Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings The Law of Segregation • When Mendel crossed contrasting, truebreeding white and purple flowered pea plants, all of the F1 hybrids were purple • When Mendel crossed the F1 hybrids, many of the F2 plants had purple flowers, but some had white • Mendel discovered a ratio of about three to one, purple to white flowers, in the F2 generation Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-3-3 EXPERIMENT P Generation (true-breeding parents) F1 Generation (hybrids) Purple flowers White flowers All plants had purple flowers F2 Generation 705 purple-flowered 224 white-flowered plants plants • Mendel reasoned that only the purple flower factor was affecting flower color in the F1 hybrids • Mendel called the purple flower color a dominant trait and the white flower color a recessive trait • Mendel observed the same pattern of inheritance in six other pea plant characters, each represented by two traits • What Mendel called a “heritable factor” is what we now call a gene Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Mendel’s Model • Mendel developed a hypothesis to explain the 3:1 inheritance pattern he observed in F2 offspring • Four related concepts make up this model • These concepts can be related to what we now know about genes and chromosomes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • The first concept is that alternative versions of genes account for variations in inherited characters • For example, the gene for flower color in pea plants exists in two versions, one for purple flowers and the other for white flowers • These alternative versions of a gene are now called alleles • Each gene resides at a specific locus on a specific chromosome Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-4 Allele for purple flowers Locus for flower-color gene Homologous pair of chromosomes Allele for white flowers • The second concept is that for each character an organism inherits two alleles, one from each parent • Mendel made this deduction without knowing about the role of chromosomes • The two alleles at a locus on a chromosome may be identical, as in the true-breeding plants of Mendel’s P generation • Alternatively, the two alleles at a locus may differ, as in the F1 hybrids Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • The third concept is that if the two alleles at a locus differ, then one (the dominant allele) determines the organism’s appearance, and the other (the recessive allele) has no noticeable effect on appearance • In the flower-color example, the F1 plants had purple flowers because the allele for that trait is dominant Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • The fourth concept, now known as the law of segregation, states that the two alleles for a heritable character separate (segregate) during gamete formation and end up in different gametes • Thus, an egg or a sperm gets only one of the two alleles that are present in the somatic cells of an organism • This segregation of alleles corresponds to the distribution of homologous chromosomes to different gametes in meiosis Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Mendel’s segregation model accounts for the 3:1 ratio he observed in the F2 generation of his numerous crosses • The possible combinations of sperm and egg can be shown using a Punnett square, a diagram for predicting the results of a genetic cross between individuals of known genetic makeup • A capital letter represents a dominant allele, and a lowercase letter represents a recessive allele Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Useful Genetic Vocabulary (on your vocab sheet) • An organism with two identical alleles for a character is said to be homozygous for the gene controlling that character • An organism that has two different alleles for a gene is said to be heterozygous for the gene controlling that character • Unlike homozygotes, heterozygotes are not true-breeding Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Because of the different effects of dominant and recessive alleles, an organism’s traits do not always reveal its genetic composition • Therefore, we distinguish between an organism’s phenotype, or physical appearance, and its genotype, or genetic makeup • In the example of flower color in pea plants, PP and Pp plants have the same phenotype (purple) but different genotypes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-6 3 Phenotype Genotype Purple PP (homozygous) Purple Pp (heterozygous) 1 2 1 Purple Pp (heterozygous) White pp (homozygous) Ratio 3:1 Ratio 1:2:1 1 The Testcross • How can we tell the genotype of an individual with the dominant phenotype? • Such an individual must have one dominant allele, but the individual could be either homozygous dominant or heterozygous • The answer is to carry out a testcross: breeding the mystery individual with a homozygous recessive individual • If any offspring display the recessive phenotype, the mystery parent must be heterozygous Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-7 TECHNIQUE Dominant phenotype, Recessive phenotype, unknown genotype: known genotype: PP or Pp? pp Predictions If PP Sperm p p P Pp Eggs If Pp Sperm p p or P Pp Eggs P Pp Pp pp pp p Pp Pp RESULTS or All offspring purple 1/2 offspring purple and 1/2 offspring white The Law of Independent Assortment • Mendel derived the law of segregation by following a single character • The F1 offspring produced in this cross were monohybrids, individuals that are heterozygous for one character • A cross between such heterozygotes is called a monohybrid cross Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Mendel identified his second law of inheritance by following two characters at the same time • Crossing two true-breeding parents differing in two characters produces dihybrids in the F1 generation, heterozygous for both characters • A dihybrid cross, a cross between F1 dihybrids, can determine whether two characters are transmitted to offspring as a package or independently Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-8 EXPERIMENT YYRR P Generation yyrr Gametes YR F1 Generation YyRr Hypothesis of dependent assortment Predictions yr Hypothesis of independent assortment Sperm or Predicted offspring of F2 generation 1/ 4 Sperm 1/ YR 1/ 2 2 yr 1/ 4 1/ 2 YR 1/ 4 1/ 4 Yr yR 1/ 4 yr YR YYRR YYRr YyRR YyRr YYRr YYrr YyRr Yyrr YyRR YyRr yyRR yyRr YyRr Yyrr yyRr yyrr YR YYRR Eggs 1/ 2 YyRr 1/ 4 Yr Eggs yr YyRr 3/ 4 yyrr 1/ 4 yR 1/ 4 Phenotypic ratio 3:1 1/ 4 yr 9/ 16 3/ 16 3/ 16 1/ 16 Phenotypic ratio 9:3:3:1 RESULTS 315 108 101 32 Phenotypic ratio approximately 9:3:3:1 • Using a dihybrid cross, Mendel developed the law of independent assortment • The law of independent assortment states that each pair of alleles segregates independently of each other pair of alleles during gamete formation • Strictly speaking, this law applies only to genes on different, nonhomologous chromosomes • Genes located near each other on the same chromosome tend to be inherited together Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Concept 14.2: The laws of probability govern Mendelian inheritance • Mendel’s laws of segregation and independent assortment reflect the rules of probability • When tossing a coin, the outcome of one toss has no impact on the outcome of the next toss • In the same way, the alleles of one gene segregate into gametes independently of another gene’s alleles Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings The Multiplication and Addition Rules Applied to Monohybrid Crosses • The multiplication rule states that the probability that two or more independent events will occur together is the product of their individual probabilities • Probability in an F1 monohybrid cross can be determined using the multiplication rule • Segregation in a heterozygous plant is like flipping a coin: Each gamete has a 12 chance of 1 carrying the dominant allele and a 2 chance of carrying the recessive allele Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-9 Rr Segregation of alleles into eggs Rr Segregation of alleles into sperm Sperm 1/ R 2 R 1/ 2 r R R Eggs 4 r 2 r 2 R 1/ 1/ 1/ 1/ 4 r r R r 1/ 4 1/ 4 • The rule of addition states that the probability that any one of two or more exclusive events will occur is calculated by adding together their individual probabilities • The rule of addition can be used to figure out the probability that an F2 plant from a monohybrid cross will be heterozygous rather than homozygous Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Solving Complex Genetics Problems with the Rules of Probability • We can apply the multiplication and addition rules to predict the outcome of crosses involving multiple characters • A dihybrid or other multicharacter cross is equivalent to two or more independent monohybrid crosses occurring simultaneously • In calculating the chances for various genotypes, each character is considered separately, and then the individual probabilities are multiplied together Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-UN1 For the cross PpYyRr x Ppyyrr what would be the chance of offspring having at least two recessive traits? You will get these results if you do Punnett squares for each trait separately: PP = ¼, Pp = ¼, pp = ¼ YY = 0, Yy = ½, yy = ½ RR = 0, Rr = ½, rr = ½ These are the genotypes that will show at least two recessive traits: Concept 14.3: Inheritance patterns are often more complex than predicted by simple Mendelian genetics • The relationship between genotype and phenotype is rarely as simple as in the pea plant characters Mendel studied • Many heritable characters are not determined by only one gene with two alleles • However, the basic principles of segregation and independent assortment apply even to more complex patterns of inheritance Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Extending Mendelian Genetics for a Single Gene • Inheritance of characters by a single gene may deviate from simple Mendelian patterns in the following situations: – When alleles are not completely dominant or recessive – When a gene has more than two alleles – When a gene produces multiple phenotypes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Degrees of Dominance • Complete dominance occurs when phenotypes of the heterozygote and dominant homozygote are identical • In incomplete dominance, the phenotype of F1 hybrids is somewhere between the phenotypes of the two parental varieties • In codominance, two dominant alleles affect the phenotype in separate, distinguishable ways Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-10-3 P Generation Red CRCR Incomplete dominance White CWCW CR Gametes CW Pink CRCW F1 Generation Gametes 1/2 CR 1/ CW 2 Sperm 1/ 2 CR 1/ 2 CW F2 Generation 1/ 2 CR Eggs 1/ 2 CRCR CRCW CRCW CWCW CW The Relation Between Dominance and Phenotype • A dominant allele does not subdue a recessive allele; alleles don’t interact • Alleles are simply variations in a gene’s nucleotide sequence • For any character, dominance/recessiveness relationships of alleles depend on the level at which we examine the phenotype Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Frequency of Dominant Alleles • Dominant alleles are not necessarily more common in populations than recessive alleles • For example, one baby out of 400 in the United States is born with extra fingers or toes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • The allele for this unusual trait is dominant to the allele for the more common trait of five digits per appendage • In this example, the recessive allele is far more prevalent than the population’s dominant allele Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Multiple Alleles • Most genes exist in populations in more than two allelic forms • For example, the four phenotypes of the ABO blood group in humans are determined by three alleles for the enzyme (I) that attaches A or B carbohydrates to red blood cells: IA, IB, and i. • The enzyme encoded by the IA allele adds the A carbohydrate, whereas the enzyme encoded by the IB allele adds the B carbohydrate; the enzyme encoded by the i allele adds neither Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-11 Allele IA IB Carbohydrate A B i none (a) The three alleles for the ABO blood groups and their associated carbohydrates Genotype Red blood cell appearance Phenotype (blood group) IAIA or IA i A IBIB or IB i B IAIB AB ii O (b) Blood group genotypes and phenotypes Polygenic Inheritance • Quantitative characters are those that vary in the population along a continuum • Quantitative variation usually indicates polygenic inheritance, an additive effect of two or more genes on a single phenotype • Skin color in humans is an example of polygenic inheritance Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-13 AaBbCc AaBbCc Sperm 1/ Eggs 1/ 8 1/ 8 1/ 8 1/ 8 1/ 1/ 8 1/ 1/ 8 8 1/ 8 1/ 64 15/ 8 1/ 1/ 8 8 8 1/ 8 1/ 8 1/ 8 8 1/ Phenotypes: 64 Number of dark-skin alleles: 0 6/ 64 1 15/ 64 2 20/ 3 64 4 6/ 64 5 1/ 64 6 Nature and Nurture: The Environmental Impact on Phenotype • Another departure from Mendelian genetics arises when the phenotype for a character depends on environment as well as genotype • The norm of reaction is the phenotypic range of a genotype influenced by the environment • For example, hydrangea flowers of the same genotype range from blue-violet to pink, depending on soil acidity Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-14 • Norms of reaction are generally broadest for polygenic characters • Such characters are called multifactorial because genetic and environmental factors collectively influence phenotype Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Concept 14.4: Many human traits follow Mendelian patterns of inheritance • Humans are not good subjects for genetic research – Generation time is too long – Parents produce relatively few offspring – Breeding experiments are unacceptable • However, basic Mendelian genetics endures as the foundation of human genetics Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Pedigree Analysis • A pedigree is a family tree that describes the interrelationships of parents and children across generations • Inheritance patterns of particular traits can be traced and described using pedigrees Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-15a Key Male Female Affected male Affected female Mating Offspring, in birth order (first-born on left) Fig. 14-15b 1st generation (grandparents) 2nd generation (parents, aunts, and uncles) Ww ww ww Ww ww ww Ww Ww Ww ww 3rd generation (two sisters) WW or Ww Widow’s peak ww No widow’s peak (a) Is a widow’s peak a dominant or recessive trait? • Pedigrees can also be used to make predictions about future offspring • We can use the multiplication and addition rules to predict the probability of specific phenotypes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Recessively Inherited Disorders • Many genetic disorders are inherited in a recessive manner Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings The Behavior of Recessive Alleles • Recessively inherited disorders show up only in individuals homozygous for the allele • Carriers are heterozygous individuals who carry the recessive allele but are phenotypically normal (i.e., pigmented) • Albinism is a recessive condition characterized by a lack of pigmentation in skin and hair Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-16 Parents Normal Aa Normal Aa Sperm A a A AA Normal Aa Normal (carrier) a Aa Normal (carrier) aa Albino Eggs • If a recessive allele that causes a disease is rare, then the chance of two carriers meeting and mating is low • Consanguineous matings (i.e., matings between close relatives) increase the chance of mating between two carriers of the same rare allele • Most societies and cultures have laws or taboos against marriages between close relatives Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Cystic Fibrosis • Cystic fibrosis is the most common lethal genetic disease in the United States,striking one out of every 2,500 people of European descent • The cystic fibrosis allele results in defective or absent chloride transport channels in plasma membranes • Symptoms include mucus buildup in some internal organs and abnormal absorption of nutrients in the small intestine Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Chapter 15 The Chromosomal Basis of Inheritance PowerPoint® Lecture Presentations for Biology Eighth Edition Neil Campbell and Jane Reece Lectures by Chris Romero, updated by Erin Barley with contributions from Joan Sharp Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Overview: Locating Genes Along Chromosomes • Mendel’s “hereditary factors” were genes, though this wasn’t known at the time • Today we can show that genes are located on chromosomes • The location of a particular gene can be seen by tagging isolated chromosomes with a fluorescent dye that highlights the gene Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-1 Concept 15.1: Mendelian inheritance has its physical basis in the behavior of chromosomes • Mitosis and meiosis were first described in the late 1800s • The chromosome theory of inheritance states: – Mendelian genes have specific loci (positions) on chromosomes – Chromosomes undergo segregation and independent assortment • The behavior of chromosomes during meiosis was said to account for Mendel’s laws of segregation and independent assortment Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-2 P Generation Yellow-round seeds (YYRR) Y Y R r R Green-wrinkled seeds ( yyrr) y y r Meiosis Fertilization y R Y Gametes r All F1 plants produce yellow-round seeds (YyRr) F1 Generation R R y r Y Y LAW OF SEGREGATION The two alleles for each gene separate during gamete formation. y r LAW OF INDEPENDENT ASSORTMENT Alleles of genes on nonhomologous chromosomes assort independently during gamete formation. Meiosis R r Y y r R Y y Metaphase I 1 1 R r Y y r R Y y Anaphase I R r Y y Metaphase II r R Y y 2 2 Y Y Gametes R R 1/ F2 Generation 4 YR y r r r 1/ 4 Y Y y r 1/ yr 4 Yr y y R R 1/ 4 yR An F1 F1 cross-fertilization 3 3 9 :3 :3 :1 Morgan’s Experimental Evidence: Scientific Inquiry • The first solid evidence associating a specific gene with a specific chromosome came from Thomas Hunt Morgan, an embryologist • Morgan’s experiments with fruit flies provided convincing evidence that chromosomes are the location of Mendel’s heritable factors Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Morgan’s Choice of Experimental Organism • Several characteristics make fruit flies a convenient organism for genetic studies: – They breed at a high rate – A generation can be bred every two weeks – They have only four pairs of chromosomes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Morgan noted wild type, or normal, phenotypes that were common in the fly populations • Traits alternative to the wild type are called mutant phenotypes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-3 Correlating Behavior of a Gene’s Alleles with Behavior of a Chromosome Pair • In one experiment, Morgan mated male flies with white eyes (mutant) with female flies with red eyes (wild type) – The F1 generation all had red eyes – The F2 generation showed the 3:1 red:white eye ratio, but only males had white eyes • Morgan determined that the white-eyed mutant allele must be located on the X chromosome • Morgan’s finding supported the chromosome theory of inheritance Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-4c CONCLUSION P Generation w+ X X w+ X Y w Eggs F1 Generation Sperm w+ w+ w+ w w+ Eggs F2 Generation w w+ Sperm w+ w+ w w w w+ Concept 15.2: Sex-linked genes exhibit unique patterns of inheritance • In humans and some other animals, there is a chromosomal basis of sex determination Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings The Chromosomal Basis of Sex • In humans and other mammals, there are two varieties of sex chromosomes: a larger X chromosome and a smaller Y chromosome • Only the ends of the Y chromosome have regions that are homologous with the X chromosome • The SRY gene on the Y chromosome codes for the development of testes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-5 X Y • Females are XX, and males are XY • Each ovum contains an X chromosome, while a sperm may contain either an X or a Y chromosome • Other animals have different methods of sex determination Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Inheritance of Sex-Linked Genes • The sex chromosomes have genes for many characters unrelated to sex • A gene located on either sex chromosome is called a sex-linked gene • In humans, sex-linked usually refers to a gene on the larger X chromosome Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Sex-linked genes follow specific patterns of inheritance • For a recessive sex-linked trait to be expressed – A female needs two copies of the allele – A male needs only one copy of the allele • Sex-linked recessive disorders are much more common in males than in females Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Some disorders caused by recessive alleles on the X chromosome in humans: – Color blindness – Duchenne muscular dystrophy – Hemophilia Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Concept 15.3: Linked genes tend to be inherited together because they are located near each other on the same chromosome • Linked genes do not follow Mendel’s law of independent assortment How Linkage Affects Inheritance • Morgan did other experiments with fruit flies to see how linkage affects inheritance of two characters • Morgan crossed flies that differed in traits of body color and wing size Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-UN1 b vg b+ vg+ Parents in testcross Most offspring b vg b vg b+ vg+ b vg or b vg b vg Fig. 15-9-4 EXPERIMENT P Generation (homozygous) Wild type (gray body, normal wings) Double mutant (black body, vestigial wings) b b vg vg b+ b+ vg+ vg+ F1 dihybrid (wild type) Double mutant TESTCROSS b+ b vg+ vg Testcross offspring b b vg vg b vg b+ vg b vg+ Wild type (gray-normal) Blackvestigial Grayvestigial Blacknormal b+ b vg+ vg b b vg vg b+ b vg vg b b vg+ vg Eggs b+ vg+ b vg Sperm PREDICTED RATIOS If genes are located on different chromosomes: 1 : 1 : 1 : 1 If genes are located on the same chromosome and parental alleles are always inherited together: 1 : 1 : 0 : 0 965 : 944 : 206 : 185 RESULTS • Morgan found that body color and wing size are usually inherited together in specific combinations (parental phenotypes) • He noted that these genes do not assort independently, and reasoned that they were on the same chromosome Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • However, nonparental phenotypes were also produced • Understanding this result involves exploring genetic recombination, the production of offspring with combinations of traits differing from either parent Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Genetic Recombination and Linkage • The genetic findings of Mendel and Morgan relate to the chromosomal basis of recombination Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Recombination of Unlinked Genes: Independent Assortment of Chromosomes • Mendel observed that combinations of traits in some offspring differ from either parent • Offspring with a phenotype matching one of the parental phenotypes are called parental types • Offspring with nonparental phenotypes (new combinations of traits) are called recombinant types, or recombinants • A 50% frequency of recombination is observed for any two genes on different chromosomes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-UN2 Gametes from yellow-round heterozygous parent (YyRr) Gametes from greenwrinkled homozygous recessive parent ( yyrr) YR yr Yr yR YyRr yyrr Yyrr yyRr yr Parentaltype offspring Recombinant offspring Recombination of Linked Genes: Crossing Over • Morgan discovered that genes can be linked, but the linkage was incomplete, as evident from recombinant phenotypes • Morgan proposed that some process must sometimes break the physical connection between genes on the same chromosome • That mechanism was the crossing over of homologous chromosomes Animation: Crossing Over Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-10a Testcross parents Black body, vestigial wings (double mutant) Gray body, normal wings (F1 dihybrid) Replication of chromosomes Meiosis I b+ vg+ b vg b vg b vg b+ vg+ b vg b+ vg+ b vg b vg b vg b vg b vg b+ vg+ b+ Meiosis I and II vg b vg+ b vg Meiosis II Recombinant chromosomes b+ vg+ b vg Eggs b+ vg b vg+ b vg Sperm Replication of chromosomes Fig. 15-10b Recombinant chromosomes Eggs Testcross offspring b+ vg+ b vg b+ vg b vg+ 944 Wild type Black(gray-normal) vestigial 206 Grayvestigial 185 Blacknormal 965 b+ vg+ b vg b+ vg b vg+ b vg b vg b vg b vg Parental-type offspring Recombinant offspring 391 recombinants Recombination 100 = 17% = frequency 2,300 total offspring b vg Sperm Mapping the Distance Between Genes Using Recombination Data: Scientific Inquiry • Alfred Sturtevant, one of Morgan’s students, constructed a genetic map, an ordered list of the genetic loci along a particular chromosome • Sturtevant predicted that the farther apart two genes are, the higher the probability that a crossover will occur between them and therefore the higher the recombination frequency Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • A linkage map is a genetic map of a chromosome based on recombination frequencies • Distances between genes can be expressed as map units; one map unit, or centimorgan, represents a 1% recombination frequency • Map units indicate relative distance and order, not precise locations of genes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Genes that are far apart on the same chromosome can have a recombination frequency near 50% • Such genes are physically linked, but genetically unlinked, and behave as if found on different chromosomes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Concept 15.4: Alterations of chromosome number or structure cause some genetic disorders • Large-scale chromosomal alterations often lead to spontaneous abortions (miscarriages) or cause a variety of developmental disorders Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Abnormal Chromosome Number • In nondisjunction, pairs of homologous chromosomes do not separate normally during meiosis • As a result, one gamete receives two of the same type of chromosome, and another gamete receives no copy Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-13-3 Meiosis I Nondisjunction Meiosis II Nondisjunction Gametes n+1 n+1 n–1 n–1 n+1 n–1 n Number of chromosomes (a) Nondisjunction of homologous chromosomes in meiosis I (b) Nondisjunction of sister chromatids in meiosis II n • Aneuploidy results from the fertilization of gametes in which nondisjunction occurred • Offspring with this condition have an abnormal number of a particular chromosome Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • A monosomic zygote has only one copy of a particular chromosome • A trisomic zygote has three copies of a particular chromosome Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Polyploidy is a condition in which an organism has more than two complete sets of chromosomes – Triploidy (3n) is three sets of chromosomes – Tetraploidy (4n) is four sets of chromosomes • Polyploidy is common in plants, but not animals • Polyploids are more normal in appearance than aneuploids Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-14 This mouse is tetraploid. He’s a bit larger than normal. Alterations of Chromosome Structure • Breakage of a chromosome can lead to four types of changes in chromosome structure: – Deletion removes a chromosomal segment – Duplication repeats a segment – Inversion reverses a segment within a chromosome – Translocation moves a segment from one chromosome to another Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-15 (a) (b) (c) (d) A B C D E F G H A B C D E F G H A B C D E F G H A B C D E F G H Deletion Duplication A B C E F G H A B C B C D E Inversion A D C B E R F G H M N O C D E Reciprocal translocation M N O P Q F G H A B P Q R F G H Human Disorders Due to Chromosomal Alterations • Alterations of chromosome number and structure are associated with some serious disorders • Some types of aneuploidy appear to upset the genetic balance less than others, resulting in individuals surviving to birth and beyond • These surviving individuals have a set of symptoms, or syndrome, characteristic of the type of aneuploidy Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Down Syndrome (Trisomy 21) • Down syndrome is an aneuploid condition that results from three copies of chromosome 21 • It affects about one out of every 700 children born in the United States • The frequency of Down syndrome increases with the age of the mother, a correlation that has not been explained Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-16 Down syndrome is caused by having three copies of chromosome 21. Aneuploidy of Sex Chromosomes • Nondisjunction of sex chromosomes produces a variety of aneuploid conditions • Klinefelter syndrome is the result of an extra chromosome in a male, producing XXY individuals • Monosomy X, called Turner syndrome, produces X0 females, who are sterile; it is the only known viable monosomy in humans Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Disorders Caused by Structurally Altered Chromosomes • The syndrome cri du chat (“cry of the cat”), results from a specific deletion in chromosome 5 • A child born with this syndrome is mentally retarded and has a catlike cry; individuals usually die in infancy or early childhood • Certain cancers, including chronic myelogenous leukemia (CML), are caused by translocations of chromosomes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-17 Normal chromosome 9 Normal chromosome 22 Reciprocal translocation Translocated chromosome 9 Translocated chromosome 22 (Philadelphia chromosome) Concept 15.5: Some inheritance patterns are exceptions to the standard chromosome theory • There are two normal exceptions to Mendelian genetics • One exception involves genes located in the nucleus, and the other exception involves genes located outside the nucleus Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Inheritance of Organelle Genes • Extranuclear genes (or cytoplasmic genes) are genes found in organelles in the cytoplasm • Mitochondria, chloroplasts, and other plant plastids carry small circular DNA molecules • Extranuclear genes are inherited maternally because the zygote’s cytoplasm comes from the egg • The first evidence of extranuclear genes came from studies on the inheritance of yellow or white patches on leaves of an otherwise green plant Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-19 • Some defects in mitochondrial genes prevent cells from making enough ATP and result in diseases that affect the muscular and nervous systems – For example, mitochondrial myopathy and Leber’s hereditary optic neuropathy Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-UN4 Sperm P generation D gametes C B A Egg E + c b a d F f The alleles of unlinked genes are either on separate chromosomes (such as d and e) or so far apart on the same chromosome (c and f) that they assort independently. This F1 cell has 2n = 6 chromosomes and is heterozygous for all six genes shown (AaBbCcDdEeFf). Red = maternal; blue = paternal. D Each chromosome has hundreds or thousands of genes. Four (A, B, C, F) are shown on this one. e C B A F e d E cb a f Genes on the same chromosome whose alleles are so close together that they do not assort independently (such as a, b, and c) are said to be linked.