Download Method to Amplify DNA Barcode Sequences from

Method to Amplify DNA Barcode Sequences from
Degraded Sources
Description
Patent Status:
PCT Application
CA2016050970
DNA Barcoding Explained | DNA barcoding allows non-experts to objectively
identify species. Just as the unique pattern of bars in a universal product code
(UPC) identifies each consumer product, a DNA barcode is a unique pattern of
DNA sequence that identifies each living organism.
License Status:
Seeking Licensee
The Challenge | DNA is a highly stable molecule that can last for thousands of
years when adequately preserved, but if left to the elements – such as light, heat
or humid conditions – it can degrade rapidly and severely. This presents a
challenge for DNA barcoding applications where material isn’t fresh or well
preserved.
Contact:
Tyler Zemlak
Tech Transfer Officer
[email protected]
519-824-4120 x53808
The Solution | Long DNA barcodes can now be recovered from trace amounts of
DNA using a method that stitches together tiny DNA fragments using nested
PCR.
Applications
Research Services | Sequence the DNA barcode region from dried or formalinfixed museum specimens.
QA/QC Testing | Authenticate highly processed food ingredients.
Wildlife Enforcement | Positively identify obfuscated items entering the global
wildlife trade.
Benefits
Low cost and customizable method with high success rates that can overcome
DNA damage caused by a variety of sources as long as DNA is still present in
sample.
Development Status
R&D work planned to increase analytical throughput and expand taxonomic
breadth.
ICHTHYOLOGY BUILDING #92
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GUELPH
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ONTARIO
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CANADA
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N1G 4T2
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519-824-4120, EXT. 58878
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