Download This project aims to identify differences in DNA structure of cells in

STAT 450 application
YORK, Elisa
PURPOSE: This project aims to identify differences in DNA structure of cells in young
vs. aged mice.
BACKGROUND: Neuron death in Alzheimer’s disease may be amplified by a loss of
protection by microglia, the immune cells of the central nervous system. Like replicating
cells, microglia become less functional as they age. Aging of cells throughout the body
can be regulated by how tightly their DNA is stored. Addition of chemical groups to the
DNA-packaging proteins can either tighten or loosen the DNA. Therefore, I will
determine if compounds that loosen DNA are capable of restoring the ability of aged
microglia to protect neurons from age-related damage.
METHODS: Using cell culture, young and aged cells will be compared for differences in
protein expression, various functions (migration, response to injury, etc.), levels of
secreted proteins, and level of DNA compaction. Both young and aged cells will also be
compared after drug treatment to alter DNA compaction.
STATISTICAL QUESTIONS:
1. Is there a difference in level of specific protein expression between young and
aged cells?
2. Does DNA compaction increase in aged cells (compared by cell counts)?
3. Is migration distance greater in young cells when compared with aged cells?
4. Does drug treatment decrease DNA compaction?