Download Annotated Bibliography: 1. Bose B, Shenoy P. Non insulin producing

Annotated Bibliography:
1. Bose B, Shenoy P. Non insulin producing cell line, MIA PaCa‐2 is rendered insulin
producing in vitro via mesenchymal epithelial transition. Journal of cellular biochemistry.
2013 Jul 1;114(7):1642-52.
In this paper Bose et al., has reported the morphology transition of the non-insulin producing
pancreatic adenocarcinoma cell line, from the mesenchymal to epithelial morphology.
Authors have changed the media condition of the cells, i.e. they kept the cells in serum free
media instead of serum containing media with N2/B27 supplements. In the result the cells
had change their morphology and became epithelial in nature. Here the most important
observation was that, the non-insulin producing pancreatic ductal adenocarcinoma cell were
producing insulin due to this media condition change. Authors have validated the expression
of the insulin and the other epithelial marker with immune cytochemistry and qRT PCR
method. This investigation thus provides a basis for using this basic developmental biology
phenomenon mesenchymal to epithelial transition as a strategy to generate a large number of
functional islets from stem cells of mesenchymal origin.
2. Bose B, Shenoy S. Pluripotent Conversion of Muscle Stem Cells Without Reprogramming
Factors or Small Molecules. Stem Cell Reviews and Reports. 2016 Feb 1;12(1):73-89.
In this paper authors have shown that, muscle derived stem cells (MDSCs) are multipotent
stem cells that can differentiate into several lineages including skeletal muscle precursor
cells, and others. Authors have reported that MDSCs from myostatin null mice (Mstn (-/-) )
can be readily induced into pluripotent stem cells without using reprogramming factors. They
also kept the MDSC in to embryonic stem cell media with LIF (leukaemia inducing factor),
those myostatin null cells had formed embryonic stem like cell, and they have formed
embryed body. They have validated the expression of LIF, Oct4, Nanog, Sox2 and SSEA-1 in
culture induced pluripotent stem cell (Cipsc) by microarray western blotting, gene
expression, and immunocytochemistry method. In addition they have injected the MDSC
(Mstn (-/-) ) in immunocompromised mice and observed the formation of embryoid body and
teratoma formation. They have proposed that lack of myostatin epigenetically reprograms the
MDSCs to become pluripotent stem cells without any other reprogrammed genes.
3. Bose B, Shenoy S. Aging induced loss of stemness with concomitant gain of myogenic
properties of a pure population of CD34+/CD45− muscle derived stem cells. The
international journal of biochemistry & cell biology. 2016 Jan 31;70:1-2.
In this study, Bose et al., has report an inverse correlation between aging and expression of
adult/mesenchymal stem cell markers and a direct correlation between aging and
myogenecity in MDSCs. They have isolated the CD34+/45- cells from the mixed population
of MDSC by the FACS and they have validated with other molecular techniques. After that,
they have transplanted those CD34+/45- cells into the old and aged mice and aged MDSCs
were able to generate a greater number of dystrophin positive myofibres, as compared to, the
young MDSCs when transplanted in muscle of dystrophic mice. Within a tissue, both
differentiated cells and adult stem cells are susceptible to intrinsic and extrinsic changes
while aging this article has also opens the possibilities of using unipotent aged MDSCs as
potential candidates for transplantation in patients with muscular dystrophies.
4. Bose B, Shenoy S. Stem cell versus cancer and cancer stem cell: intricate balance decides
their respective usefulness or harmfulness in the biological system. Journal of Stem Cell
Research & Therapy. 2014 Feb 24;2014.
In this paper, authors have reviewed on the comparison between embryonic stem cell and
cancer stem cells. They have nicely depicted the properties of the stem cells and their
molecular mechanisms and characteristic and markers they have reviewed on cancer stem
cells. Cancer stem cells are the main culprit in the tumor progression and can be said it is the
tumor inducer cells, as per their review. They have also mentioned the aggressive ness of the
cancer stem cells as compare to embryonic stem cell. In the last they have mentioned the
techniques or strategies to target the cancer stem cells by the various approaches, namely
pathway based target, surface marker based, anticancer drug based and shRNA based cancer
therapeutics. They are suggesting that, if we can target the CSCs from a mixed wild type
population it will a nice therapeutic approach for the future cancer therapy.
5. Bose B, Gour RR, Motiwale L, Gupta S, Rao KV. Hyperphosphorylation of
extracellularregulated kinase 2 (ERK2) and inhibition of JNK2 phosphorylation are
associated with increased S-phase during transformation of Syrian hamster embryo cells by
Malachite Green. Cell biology international. 2004 Dec 31;28(12):875-83.
In this study the authors have reported that hyperphosphorylation of ERK2, decreased JNK2
phosphorylation and an increase in S-phase cells seems to be the early changes associated
with the MG induced malignant transformation of SHE cells in primary culture. Malachite
Green (MG), consisting of green crystals with a metallic lustre, is highly soluble in water,
cytotoxic to various mammalian cells and also acts as a liver tumour promoter. In view of its
industrial importance and possible exposure to human beings, MG poses a potential
environmental health hazard. But in this study authors have depicted that the mitogen
activated protein (MAP) kinase signal transduction pathway in preneoplastic cells induced by
malachite green.
Summary:
Here in the all five papers, the authors are mainly focusing on basic research on stem cell
biology and cancer biology. Lastly, they are focusing on the multidisciplinary research on
cancer stem-cell biology too. Their idea of research is to observe the basic developmental
biology and how it’s related to cancer biology and the comparative study between the cancer
cell and cancer stem cell; stem cell and cancer stem cell biology and its associated molecular
mechanisms and characteristics. Their aim is also to modulate the cancer stem cell and its
associated mechanisms, as a therapeutic strategy with cutting age technologies and other
inducible factors like drug. They want to transform the basic research to clinical trials with
consecutive translational values.