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Exercise Follow up and Conclusion for: DNA Fingerprinting and Big Idea #3 – Genetics Information and Transfer – Biotech Restriction Enzyme Analysis of DNA I. Read page S120 in Big Idea #3 – Genetics Information and Transfer – Biotech Restriction Enzyme Analysis of DNA. What is ‘bp length’ and what is it used for in this type of lab work? What is important about the molecular weight of a DNA fragment and its ability to navigate a gel? What is a DNA standard and why is it necessary? II. Read over the handout on ESTIMATING SIZES OF DNA BANDS BY SEMI-LOG PLOT (Science Education Partnership. Pay careful attention to pages 1, 2 and 4. III. Now, Read the rest of the information on S120 – S121 in Big Idea #3 – Genetics Information and Transfer – Biotech Restriction Enzyme Analysis of DNA. Using this data, complete the exercise as directed. Perform the appropriate measurements for all fragments on the Ideal Gel on page S121n and record on the chart in this handout. Using the data for fragment HINDIII, plot your line of ‘Best fit’ on the semi-log paper blank provided for you with the handout in II. Make the Best Fit line RED. Determine the BP lengths for the other fragments using your graph and fill in the rest of your chart. HINDIII Distance BP Length Traveled *27491 *23130 9416 6557 4361 2322 2027 **564 **125 BAMHI Distance BP Length Traveled ECORI Distance BP Length Traveled