Download Exercise Follow up and Conclusion for: DNA Fingerprinting and Big

Exercise Follow up and Conclusion for:
DNA Fingerprinting and Big Idea #3 – Genetics Information and Transfer – Biotech
Restriction Enzyme Analysis of DNA
I.
Read page S120 in Big Idea #3 – Genetics Information and Transfer – Biotech Restriction
Enzyme Analysis of DNA.
 What is ‘bp length’ and what is it used for in this type of lab work?

What is important about the molecular weight of a DNA fragment and its ability to
navigate a gel?

What is a DNA standard and why is it necessary?
II.
Read over the handout on ESTIMATING SIZES OF DNA BANDS BY SEMI-LOG PLOT (Science
Education Partnership. Pay careful attention to pages 1, 2 and 4.
III.
Now, Read the rest of the information on S120 – S121 in Big Idea #3 – Genetics Information
and Transfer – Biotech Restriction Enzyme Analysis of DNA.

Using this data, complete the exercise as directed. Perform the appropriate
measurements for all fragments on the Ideal Gel on page S121n and record on the
chart in this handout.

Using the data for fragment HINDIII, plot your line of ‘Best fit’ on the semi-log paper
blank provided for you with the handout in II. Make the Best Fit line RED.
Determine the BP lengths for the other fragments using your graph and fill in the
rest of your chart.

HINDIII
Distance BP Length
Traveled
*27491
*23130
9416
6557
4361
2322
2027
**564
**125
BAMHI
Distance
BP Length
Traveled
ECORI
Distance
BP Length
Traveled