Download RTP DNA/RNA Virus Mini Kit

RTP DNA/RNA Virus Mini Kit
Starting Material : serum, urine, plasma, cerebrospinal fluid, cell culture
supernatants
and other cell-free body fluids as well as swabs or tissue biopsies
The RTP® DNA/RNA Virus Mini Kit is the ideal tool for simultaneous isolation of
DNA and from up to 200 μl serum, urine, plasma, cerebrospinal fluid, cell culture
supernatants and other cell-free body fluids.
Specification :
Amount of starting material
-
200 µl serum, plasma, cell-free body fluids
1-10 mg tissue
200 µl cell culture supernatant
swab
time for preparation
- about 35 min (incl. lysis time)
Yield
- depends on starting material
Downstream-Application
-
Technology
- membrane adsorption
- RTP® technology
Amplification
qualitativ or quantitativ virus detection
quantitativ RT-PCR
virus diagnostic
Applications :
Virus RNA was isolated from 200 µl HCV infected human
serum sample using the RTP® DNA/RNA Virus Mini Kit
(serum contain 50.000 HCV copies/ml, detected by Cobas
Amplicor Monitor® HCV Roche, Mannheim). 5 µl out of 60 µl
of the eluted virus RNA was used in a in-house HCV-RT-PCR
(EZ buffer/rTth). The RT-PCR was done on a GeneAmp 5700
SDS quantified against a Roboscreen Hepatitis C Virus Control
Strip, coated with defined amounts of in vitro synthezised
control HCV-RNA used for the generation of a standard graph
(10....2500, 1000, 250 copies per tube).
(Data kindly provided by Dr. Mona Amin, Roboscreen GmbH, Leipzig)
Advantages :
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remarkable reduction of handling steps and
processing time by using preformulated
and coated reagents
addition of internal standards is not necessary
highly efficient recovery of high-quality viral DNA
and RNA from different sources,
allowing very sensitive virus detection
integrated Internal Controls allow the exclusion
of false negative results
simple storage at room temperature
Procedure:
Using the RTP® DNA/RNA Virus Mini Kit, the sample
will be lysed in the prefilled Extraction Tubes
containing preformulated lysis reagents (nonchaotropic
lysis buffer, Proteinase K) and coated carrier nucleic
acids as well as Internal Controls. The lysis reagents
furthermore stabilize the viral nucleic acids and enhance
the selective DNA/RNA adsorption to the membrane.
Before the viral nucleic acids are eluted, the
membranes are efficiently washed in order to
completely remove all PCR inhibitors.
By using the prefilled Extraction Tubes, the
preparation of carrier nucleic acid solution or Proteinase
K solution, as well as the pipetting of different
components to the sample during the lysis, are no
longer necessary. This reduces handling steps and
processing time to a minimum and makes the system
very simple. As a result of high extraction efficiency, the
kit realizes high detection sensitivities. Purified nucleic
acids are free of proteins, nucleases and other
impurities and are ready to use for any downstream
application, such as qualitative or quantitative virus
detection.
Validated human and animal viruses:
SARS, HCV, HIV, HBV, EBV, CMV, Influenza virus
(human, poultry), Herpes virus (Aujeszky disease),
NLV, RSV, TTV, Enterovirus, Circovirus, BVDVPestiviruses