Download The subject of the offer are unique sequences of single

ANTI-HISTIDINE TAG DNA APTAMERS FOR BIOTECHNOLOGICAL
APPLICATIONS
(TECHNOLOGY OFFER P-187)
The subject of the offer are unique sequences of single‐stranded DNA aptamers with high affinity to any molecule (peptide, protein or DNA derivative) containing His‐Tag and their application in biotechnology. Prokaryotic and eukaryotic expression systems for the production of recombinant proteins are used on a large scale in scientific research. The genetically engineered fusion tags, e.g. histidine tag (His‐Tag) are used for fast and efficient purification of the desired recombinant proteins. The high affinity of His‐Tag to the certain metal ions (cobalt, copper, nickel or zinc) enables the application of chromatographic media with the above mentioned ions for the purification or immobilization of His‐Tagged proteins. His‐Tag recombinant protein can be detected in western blotting using anti‐His mono‐ and polyclonal antibodies. Short His‐Tag can be used to investigate protein‐
protein interactions in techniques such as pull down assays, immunoprecpitation, co‐immunoprecipitation or Far‐Western blot analysis. DNA aptamers are single‐stranded DNA oligonucleotides of approximately 4‐100 nucleotides that exhibit a high affinity and specificity to their corresponding ligand. An optimal binding between target molecule and selected aptamer is possible due to the rich secondary and tertiary structures of the latter one. The subject of the offer are unique sequences of single‐stranded DNA aptamers that recognize His‐Tag and their application in biotechnology in analysis of His‐
Tagged molecules (peptides, proteins or DNA derivatives). The offered DNA aptamers can be used for analysis of molecules containing a His‐
Tag, and in particular to: 
application as a agonist for anti‐histidine antibodies; 
purification and detection of the target molecules containing a His‐Tag. In addition to the above applications, DNA aptamers can be used as molecules with attached tags, especially fluorescent, and in particular to label the molecular targets containing His‐Tag. This type of molecules can be successfully used e.g. to study the interaction between proteins using spectroscopic techniques. The offered Anti‐Histidine Tag DNA aptamers for biotechnological applications is patent pending. Further research and development of the invention are continued at the Faculty of Biochemistry, Biophysics and Biotechnology of the Jagiellonian University. Currently the Centre for Innovation, Technology Transfer and University Development (CITTRU) is looking for entities interested in commercial application of the invention. More information:
PhD Klaudia Polakowska
phone. 12 663 3832, fax: 12 663 3831
Technology Transfer Officer
e-mail: [email protected]
CITTRU, Jagiellonian University
www.cittru.uj.edu.pl