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0022-3565/00/2942-0753$03.00/0 THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS Copyright © 2000 by The American Society for Pharmacology and Experimental Therapeutics JPET 294:753–761, 2000 /2467/838126 Vol. 294, No. 2 Printed in U.S.A. Bioactivation of Selenocysteine Se-Conjugates by a Highly Purified Rat Renal Cysteine Conjugate -Lyase/Glutamine Transaminase K1 JAN N. M. COMMANDEUR, IOANNA ANDREADOU,2 MARTIJN ROOSEBOOM, MARCEL OUT, LAURENS J. DE LEUR, ED GROOT, and NICO P. E. VERMEULEN Leiden/Amsterdam Center for Drug Research, Division of Molecular Toxicology, Department of Pharmacochemistry, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands Accepted for publication April 19, 2000 This paper is available online at http://www.jpet.org The conjugation of electrophilic substrates to glutathione (GSH) and subsequent disposition of the GSH S-conjugates formed are mediated by a large number of different enzymes and transport systems present in various tissues (Commandeur et al., 1995). One of the pathways involved in the catabolism of GSH-conjugates is the -elimination reaction of the corresponding cysteine S-conjugates by cysteine conjugate -lyases (-lyases), resulting in the formation of ammonia, pyruvic acid, and thiol compounds. In the case of glutathione S-conjugates of halogenated alkenes, the thiols formed by -lyase may rearrange rapidly to chemically highly reactive intermediates, such as thionoacyl halides, thiiranes, and/or thioketenes (Dekant et al., 1988; Commandeur et al., Received for publication January 6, 2000. 1 Financial support was provided by the European Science Foundation. 2 I.A. was a visiting scientist from School of Pharmacy of the Aristotelian University of Thessaloniki, Greece. cated by the rapid consumption of ␣-keto-␥-methiolbutyric acid, purified -lyase/GTK also catalyzed transamination reactions, which appeared to even exceed that of -elimination. The corresponding sulfur analogs also showed significant transamination but were -eliminated at an extremely low rate. Comparison of the obtained enzyme kinetic data of purified -lyase/ GTK with previously obtained data from rat renal cytosol showed a poor correlation. By determining the activity profiles of cytosolic fractions applied to anion exchange fast protein liquid chromatography and gel filtration chromatography, the involvement of multiple enzymes in the -elimination of selenocysteine Se-conjugates in rat renal cytosol was demonstrated. The identity and characteristics of these alternative selenocysteine conjugate -lyases, however, remain to be established. 1996). The combination of active uptake mechanisms and a relatively high activity of -lyase in the kidney may explain the relatively selective nephrotoxicity of many halogenated alkenes in rodents. A number of studies by the group of Elfarra have demonstrated that the biochemical basis of this kidney selectivity may also be applied to target pharmacologically active thiol-containing antitumor agents, such as mercaptopurine and thioguanine, to the kidney for the treatment of renal cell carcinoma (Hwang and Elfarra, 1989, 1991; Elfarra and Hwang, 1993; Elfarra et al., 1995). More recently, the structurally strongly related selenocysteine Seconjugates were proposed as alternative prodrugs to target pharmacologically selenol compounds to the kidney by local -lyase enzyme systems (Andreadou et al., 1996). Although the above-mentioned prodrugs appear to be activated by renal subcellular fractions, the enzymes that are actually involved in these -elimination reactions have not ABBREVIATIONS: GSH, glutathione; -lyase, cysteine S-conjugate -lyase; GTK, glutamine transaminase K; KMB, ␣-keto-␥-methiolbutyric acid; OPD, o-phenylene diamine; FPLC, fast protein liquid chromatography; BTC, S-(benzothiazolyl)-L-cysteine; 1,2-DCV-Cys, S-(1,2-dichlorovinyl)-Lcysteine; TFE-Cys, S-(1,1,2,2-tetrafluoroethyl)-L-cysteine; PMSF, phenylmethylsulfonyl fluoride; CTFE-Cys, S-(2-chloro-1,1,2-trifluoroethyl)-Lcysteine; DCDFE-Cys, S-(2,2-dichloro-1,1-difluoroethyl)-L-cysteine; MALDI-TOF, matrix-assisted laser desorption time-of-flight. 753 Downloaded from jpet.aspetjournals.org at ASPET Journals on August 3, 2017 ABSTRACT Selenocysteine Se-conjugates have recently been proposed as potential prodrugs to target pharmacologically active selenol compounds to the kidney. Although rat renal cytosol displayed a high activity of -elimination activity toward these substrates, the enzymes involved in this activation pathway as yet have not been identified. In the present study, the possible involvement of cysteine conjugate -lyase/glutamine transaminase K (lyase/GTK) in cytosolic activity was investigated. To this end, the enzyme kinetics of 15 differentially substituted selenocysteine Se-conjugates and 11 cysteine S-conjugates was determined using highly purified rat renal -lyase/GTK. The results demonstrate that most selenocysteine Se-conjugates are -eliminated at a very high activity by purified -lyase/GTK, implicating an important role of this protein in the previously reported -elimination reactions in rat renal cytosol. As indi- 754 Commandeur et al. Experimental Procedures Materials. ␣-Keto-␥-methiolbutyric acid (KMB), -chloro-L-alanine, S-methyl-L-cysteine, S-ethyl-L-cysteine, S-benzyl-L-cysteine, and a diagnostic kit for aspartate aminotransferase (DG158-K) were purchased from Sigma Chemical Co. (St. Louis, MO). Amino-oxyacetic acid and phenylmethylsulfonyl fluoride (PMSF) were obtained from Aldrich Chemie (Brussels, Belgium). 4-Methoxythiophenol was obtained from Fluka (Buchs, Switzerland). S-(4-Methylbenzyl)-L-cysteine was purchased from Advanced Chemtech (Louisville, KY). S-(4Methoxybenzyl)-L-cysteine was purchased from Bachem Feinchemikalien AG (Bubendorf, Switzerland). 3 Due to a typing error, Abraham and Cooper (1996) originally reported a mass of Mr 45,800 for this protein. The correct mass of the protein, based on its cDNA sequence, is Mr 48,500 (D. G. Abraham, personnel communication). Fig. 1. Concept of activation of cysteine S-conjugates and selenocysteine Se-conjugates to pharmacologically active thiols and selenols, respectively. Selenocysteine Se-conjugates and L-selenocystine were prepared as described by Andreadou et al. (1996). S-Allyl-L-cysteine was prepared according to Freeman et al. (1994). S-(1,2-Dichlorovinyl)-Lcysteine (1,2-DCV-Cys) was synthesized as described by McKinney et al. (1959). S-(1,1,2,2-Tetrafluoroethyl)-L-cysteine (TFE-Cys), S-(2chloro-1,1,2-trifluoroethyl)-L-cysteine (CTFE-Cys), and S-(2,2-dichloro-1,1-difluoroethyl)-L-cysteine (DCDFE-Cys) were synthesized as described by Commandeur et al. (1988). Synthesis of Se-Allyl-L-selenocysteine. L-Selenocystine (1.5 mmol, 500 mg) was dissolved in 8 ml of 0.5 N NaOH and 2 ml of ethanol. At 0°C, 0.4 g (15 mmol) of NaBH4 was added while the reaction mixture was stirred. The mixture was allowed to reach room temperature, during which the color of the solution changed from yellow to colorless, After cooling again to 0°C, 4 ml of 2 N NaOH and 6 mmol of allylbromide were added, and the mixture was stirred for 3 h at room temperature. Concentrated HCl was added until pH 5 to 6 and cooled at 4°C. Se-Allyl-L-cysteine precipitated as a yellowish solid. The 1H NMR spectrum obtained was almost identical with that of S-allyl-L-cysteine (17): 1H NMR (D2O, Na2CO3): ␦ (ppm) 2.95 to 3.22 (2H, m, CH2-CH-NH2), 3.30 (2H, d, CH2ACH-CH2), 4.25 to 4.40 (1H, d of d, CH2-CH-NH2), 5.05 to 5.25 (2H, m, CH2ACH-CH2), 5.80 to 6.08 (1H, m, CH2ACH-CH2). Purification of Rat Renal Cysteine Conjugate -Lyase/GTK. -Lyase/GTK was purified from rat renal cytosol obtained from male Wistar rats (160 –250 g) supplied by Harlan (Zeist, the Netherlands). The procedure used is described in detail by Yamauchi et al. (1993) and yielded a highly purified enzyme that was 1000-fold enriched according to its increased specific activity with S-(1,2-dichlorovinyl)L-cysteine (1,2-DCV-Cys) as a substrate. Matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry analysis of this protein revealed a single MH⫹ mass of Mr 47,700 ⫾ 130,4 which is consistent with the mass as predicted from the sequence of -lyase/ GTK as determined by Perry et al. (1993). The purified -lyase/GTK obtained was dissolved in 50 mM TrisHCl buffer, pH 8.0, and was stored in 100-l fractions of 100 g/ml at ⫺20°C. When stored under these conditions, no significant decrease in specific activity toward 1,2-DCV-Cys was observed after 2 years. Enzymatic Incubations. Selenocysteine Se-conjugates and cysteine S-conjugates were incubated with the purified -lyase in 50 mM Tris-HCl buffer, pH 8.6, and at a temperature of 37°C. Unless stated otherwise, the final concentration of enzyme was 1 g/ml. The cofactor KMB was added at a final concentration of 0.2 mM, which allows assessment of transamination reaction by measuring KMB consumption (see later). After 2 min of preincubation at 37°C, incubations were started by the addition of a 10-fold concentrated enzyme solution. -Elimination reactions were assessed by determining the formation of pyruvic acid. To correct for background production of pyruvic acid, parallel incubations were always performed in absence of enzyme. All incubations were always performed in duplicate. Specific activities of transamination of all conjugates were determined at a substrate concentration of 0.5 mM. Transamination re4 MALDI-TOF mass spectrometry measurements were carried out on a VISION 2000 (Finnigan MAT, Bremen, Germany) (for details, see Jespersen et al., 1995). Samples containing 125 fmol of -lyase/GTK in 2,5-dihydroxybenzoic acid as matrix were irradiated with a nitrogen laser at 337 nm. Mass spectra were accumulated for 25 laser shots fired at the same spot on the sample surface. Downloaded from jpet.aspetjournals.org at ASPET Journals on August 3, 2017 yet been identified. To elucidate the possibilities and limitations of this prodrug concept, however, the identity and tissue distribution of the enzymes involved in the activation of cysteine S-conjugates and selenocysteine Se-conjugates remain to be characterized. Three major cysteine conjugate -lyase enzymes have been identified in rat kidney cytosol (Cooper, 1998). All are pyridoxal 5⬘-phosphate-dependent enzymes. One cytosolic -lyase in the kidney appeared to be identical with glutamine transaminase K (GTK), based on its composition and enzyme kinetic properties (Stevens et al., 1986). Originally denoted as GTK, this enzyme was isolated more than 25 years ago (Cooper and Meister, 1974). -Lyase/GTK is a dimeric protein consisting of two identical subunits of Mr 47,470. Recent cloning studies revealed that -lyase/GTK is also identical with kynurenine aminotransferase (Perry et al., 1993; Mosca et al., 1994). More recently, a closely related cysteine conjugate -lyase, with subunits of a slightly higher3 molecular weight, 48,500, was characterized with considerable overlap in substrate selectivity (Abraham and Cooper, 1996). Finally, a -lyase enzyme with high molecular weight (330,000) was demonstrated to be present in both cytosolic and mitochondrial fractions. This third -lyase has different enzyme characteristics compared with -lyase/GTK as demonstrated by its ability to convert leukotriene E4 and 5⬘-S-cysteinyldopamine and by its lower specific activity toward cysteine conjugates of halogenated alkenes (Abraham et al., 1995). Recently, we demonstrated that replacing the sulfur of cysteine S-conjugates by a selenium atom resulted in a dramatic increase in -elimination activity in rat renal cytosol (Andreadou et al., 1996). Therefore, selenocysteine Se-conjugates were proposed as alternative prodrugs to target pharmacologically active selenol compounds to the kidney (Fig. 1). The aim of the present investigation was to study whether selenocysteine Se-conjugates are substrates for purified rat renal -lyase/GTK. Next to the selenocysteine Se-conjugates, a number of corresponding cysteine S-conjugates were tested as substrates for purified rat renal -lyase/GTK as well. Because the enzyme kinetics obtained with the purified enzyme showed a relatively poor correlation with results previously obtained with rat renal cytosol, the possible involvement of multiple enzymes was studied by determining the activity profiles after fractionation of rat renal cytosol by two different chromatographic methods: anion exchange chromatography and gel permeation chromatography. Vol. 294 2000 755 cytosolic protein) was applied to the Mono-Q column, which was equilibrated with the same buffer as used for dialysis. The column was eluted at a flow rate of 1 ml/min. After an initial 10 min of elution with buffer A, a linear gradient was started by mixing with buffer B (buffer A containing 900 mM sodium chloride). Fifty minutes after the start of the gradient, the eluent reached 100% buffer B. During chromatography, elution of proteins was monitored by UV detection at 280 nm. From the start of the FPLC, 40 fractions of 2 ml were collected and stored at ⫺20°C until analysis for enzyme activity. Activity profiles of -elimination were determined by incubating all fractions with five different substrates: CTFE-Cys, Se-phenyl-Lselenocysteine, Se-allyl-L-selenocysteine, Se-isopropyl-L-selenocysteine, and Se-(4-methylbenzyl)-L-selenocysteine. Then, 15 l of the FPLC fractions was added to 135 l of 50 mM Tris-HCl buffer, pH 8.6, containing 2 mM substrate and 0.2 mM KMB. Incubations were performed for 20 min at 37°C, after which the reaction was terminated by adding 500 l of 12 mM OPD in 3 N HCl. After 60 min of derivatization at 60°C, the samples were analyzed by HPLC, as described earlier. Elution of aspartate aminotransferase, which was previously proposed as an alternative -eliminating enzyme (Kato et al., 1996), was monitored spectrophotometrically by coupling oxaloacetate formation to NADH oxidation with malate dehydrogenase, using the diagnostic kit from Sigma Chemical Co. Fractionation of Rat Kidney Cytosol by Gel Permeation Chromatography. Rat renal cytosol (5 ml) was applied to a HiLoad 16/60 Superdex 200 column (Pharmacia Biotech) and eluted at a flow rate of 0.5 ml/min with 50 mM sodium phosphate buffer, pH 7.4, containing 150 mM sodium chloride and 40 M PMSF. After 65 min, representing the time necessary to elute the dead volume, fractions of 0.5 ml were collected and placed on ice. Elution of proteins was monitored continuously by UV detection at 280 nm. Activities were determined using Se-(4-methylbenzyl)-L-selenocysteine and CTFECys as substrates. Then, 15 l of the fractions was added to 135 l of 50 mM Tris-HCl buffer, pH 8.6, containing substrate and 0.5 mM KMB. Incubations were performed for 20 min at 37°C, after which the reaction was terminated by adding 500 l of 12 mM OPD in 3 M HCl. After 60 min of derivatization at 60°C, the samples were analyzed by HPLC, as described earlier. Retention times of gel permeation column were calibrated by eluting a mixture of marker proteins of known molecular weight. Observed retention times were ␣-lactalbumin (14,200 Da), 302 min; carbonic anhydrase (29,000 Da), 257 min; chicken egg albumin (45,000 Da), 188 min; BSA monomer (66,000 Da), 177 min; BSA dimer (132,000 Da), 161 min; urease trimer (272,000 Da), 146 min; and urease hexamer (545,000 Da), 125 min. Results Time Course of Biotransformation of Cysteine S-Conjugates and Selenocysteine Conjugates. A significant time-dependent formation of pyruvic acid was observed on incubation of purified -lyase/GTK with cysteine S-conjugates of halogenated alkenes and with various selenocysteine Se-conjugates (Fig. 2). Because pyruvate formation significantly deviated from linearity after approximately 5 min, the enzyme kinetic parameters Km and kcat for the conjugates were based on the specific activities obtained using incubation times of 5 min. In the present incubation systems, the cofactor KMB is added to reactivate -lyase/GTK, which is converted to the PMP form by a concurrent transamination-reaction route (Stevens et al., 1986). The rate of KMB consumption therefore reflects the rate of transamination reactions. As shown in Fig. 1, the cofactor KMB is consumed during the incuba- Downloaded from jpet.aspetjournals.org at ASPET Journals on August 3, 2017 actions was assessed by determining consumption of the ␣-keto acid cofactor KMB as described previously (Cooper and Meister, 1974; Stevens et al., 1986). This approach precludes the necessity to develop standards and analytical assays for all individual ␣-keto acid products formed from the conjugates studied. Both pyruvic acid and KMB can be assessed in a single assay after derivatization with the ␣-keto acid-reagent o-phenylene diamine (OPD), followed by analysis by HPLC equipped with fluorescence detection (Stijntjes et al., 1992). In the case of selenocysteine Se-conjugates, specific activities of the transaminase pathway were determined by incubation for 5 min at 37°C in presence of 1 g/ml -lyase/GTK (final concentration). To obtain significant KMB consumption with cysteine S-conjugates, these compounds were incubated for 30 min at 37°C in presence of 2 g/ml -lyase/GTK. Time Course of Enzyme Reactions. Before determining enzyme kinetics, the time course of product formation was determined to assess the linearity of the -elimination reactions. To this end, incubations were performed at an incubation volume of 2 ml. After starting the reaction by the addition of 200 l of 10 g/ml enzyme solution, samples of 100 l were taken from the incubation at several time points and mixed with 500 l of OPD solution (12 mM OPD in 3 M HCl) in 1-ml Eppendorf cups. The Eppendorf cups were closed and heated for 60 min at 60°C to complete the derivatization reaction (Stijntjes et al., 1992). To the resulting solutions, 600 l of HPLC eluent (45% methanol, 54% water, 1% acetic acid) was added and transferred to HPLC vials for automated HPLC analysis (see later). Enzyme Kinetic Parameters. Enzyme kinetic parameters (Km and kcat) of conjugates were determined by incubating substrates at six to eight concentrations ranging from 0.05 to 4 mM. Due to their poor solubility, the conjugates with phenyl and benzyl substituents were incubated at concentrations up to 2 mM. Corrections for nonenzymatic degradation were made by performing parallel incubations in absence of enzyme. All incubations were performed at 37°C and at a total volume of 300 l and were started by adding 30 l of 10 g/ml purified -lyase/GTK. The final concentration of KMB in all incubations was 0.2 mM. Reactions were stopped after 5 min of incubation by the addition of 1 ml of 12 mM OPD in 3 M HCl. The resulting mixtures were subsequently heated for 60 min at 60°C. After this derivatization reaction, a volume of 600 l was mixed with 600 l of HPLC eluent (45% methanol, 54% water, 1% acetic acid) and transferred to HPLC vials for automated HPLC analysis (see later). HPLC Analysis of Pyruvic Acid and KMB. HPLC vials were placed in a Waters 707 Autoinjector cooled at 4°C. Then, 100-l samples were injected at intervals of 20 min. The analytes were chromatographed on two ChromSpher C18 columns (5-m particles, 100 ⫻ 4.6 mm; Chrompack, Bergen op Zoom, The Netherlands), which was eluted isocratically with the above-mentioned HPLC eluent at a flow rate of 0.4 ml/min. Detection of derivatized pyruvic acid and KMB was accomplished with a Jasco fluorescence detector (model 821-FP) set at an excitation wavelength of 336 nm and an emission wavelength of 420 nm. Chromatograms were analyzed using the Class VP 4.1 software package of Shimadzu (Columbia, MD). Under these conditions, retention times of derivatized pyruvic acid and KMB were 5.8 and 14.9 min, respectively. For quantification of the analytes, calibration curves were constructed by derivatizing known concentrations (ranging from 5 to 200 M) of pyruvic acid and KMB in 50 mM Tris-HCl, pH 8.6, with 12 mM OPD in 3 M HCl. After heating for 60 min at 60°C, samples were treated and analyzed in the same manner as described above. Fractionation of Rat Kidney Cytosol by Anion Exchange Fast Protein Liquid Chromatography (FPLC). Rat kidney cytosol was fractionated by FPLC using a Mono-Q anion exchange column (Pharmacia Biotech, Uppsala, Sweden). Before application to the column, rat kidney cytosol was dialysed for 20 h against 20 mM triethanol-HCl buffer, pH 7.45, containing 0.1 mM EDTA and 40 M PMSF (buffer A). After dialysis, this enzyme fraction was filtered using a 0.2-m Schlauer filter, and 0.5 ml (containing 19 mg of Bioactivation of Se-Conjugates by -Lyase/GTK 756 Commandeur et al. Vol. 294 Downloaded from jpet.aspetjournals.org at ASPET Journals on August 3, 2017 Fig. 2. Time course of pyruvic acid formation and KMB consumption in incubation of purified cysteine conjugate -lyase/GTK (1 g/ml) with 1 mM cysteine S-conjugates (top) or 1 mM selenocysteine Se-conjugates (middle and bottom). F, pyruvic acid; E, KMB. Error bars represent difference between two data points. tions to a different extent. In the incubations with TFE-Cys and CTFE-Cys as substrates, the concentration of KMB was only decreased from 200 M (initial concentration) to 175 to 180 M. In the incubations of the selenocysteine Se-conjugates, however, the consumption of KMB appeared to be significantly higher, leading to a decrease of more than 70% during a 30-min incubation period. Substrate Selectivity and Kinetics of Purified -Lyase/GTK. The specific activities and enzyme kinetic parameters of purified -lyase/GTK toward 11 cysteine S- Bioactivation of Se-Conjugates by -Lyase/GTK 2000 757 TABLE 1 Enzyme kinetic parameters of purified rat renal cysteine conjugate beta-lyase/glutamine transaminase K toward differentially substituted cysteine S-conjugates (X ⫽ S) and selenocysteine Se-conjugates (X ⫽ Se) Beta-Elimination Reaction X-Bound Substituent Specific Activity Methyl Ethyl n-Propyl i-Propyl n-Butyl Allyl TFE CTFE DCDFE 1,2-DCV Benzyl 4-Methoxybenzyl 4-Chlorobenzyl 3,4-Dichlorobenzyl Phenyl 4-Methylphenyl 4-Methoxyphenyl 4-Chlorophenyl Se S Se S Se Se Se Se S S S S S Se S Se S Se S Se Se Se S Se Se Se a Km kcat kcat/Km Specific Activitya nmol/min 䡠 mg mM min⫺1 min⫺1 䡠 mM⫺1 nmol/min 䡠 mg 1610 ⫾ 40 N.D. 3580 ⫾ 210 122 ⫾ 35 4330 ⫾ 380 4410 ⫾ 400 1430 ⫾ 520 4790 ⫾ 630 146 ⫾ 34 11,240 ⫾ 3120 6260 ⫾ 860 4230 ⫾ 290 4120 ⫾ 460 1110 ⫾ 570 20 ⫾ 12 10,220 ⫾ 3210 N.D. 8300 ⫾ 1210 42 ⫾ 22 3780 ⫾ 1800 9190 ⫾ 1500 9130 ⫾ 1300 53 ⫾ 30 136 ⫾ 12 25 ⫾ 15 65 ⫾ 10 5.0 ⫾ 2.5 N.D. 3.0 ⫾ 1.2 N.D. 0.94 ⫾ 0.31 0.44 ⫾ 0.16 0.26 ⫾ 0.05 1.19 ⫾ 0.23 N.D. 1.24 ⫾ 0.31 0.72 ⫾ 0.25 0.51 ⫾ 0.24 0.51 ⫾ 0.19 0.81 ⫾ 0.34 N.D. 0.66 ⫾ 0.21 N.D. 0.66 ⫾ 0.21 N.D. 0.70 ⫾ 0.04 0.64 ⫾ 0.18 0.92 ⫾ 0.20 N.D. N.D. N.D. N.D. 1790 ⫾ 480 N.D. 2500 ⫾ 950 N.D. 990 ⫾ 110 790 ⫾ 90 210 ⫾ 150 1950 ⫾ 230 N.D. 3520 ⫾ 520 1460 ⫾ 160 985 ⫾ 120 987 ⫾ 89 249 ⫾ 32 N.D. 2270 ⫾ 340 N.D. 2270 ⫾ 340 N.D. 1170 ⫾ 450 2020 ⫾ 640 2430 ⫾ 360 N.D. N.D. N.D. N.D. 340 ⫾ 30 N.D. 850 ⫾ 100 N.D. 1200 ⫾ 360 1810 ⫾ 230 810 ⫾ 290 1640 ⫾ 230 N.D. 3090 ⫾ 210 2030 ⫾ 145 1669 ⫾ 162 1930 ⫾ 250 406 ⫾ 150 N.D. 3430 ⫾ 450 N.D. 3170 ⫾ 190 N.D. 1670 ⫾ 320 3160 ⫾ 120 2740 ⫾ 490 N.D. N.D. N.D. N.D. 7400 ⫾ 1550 1370 ⫾ 250 11,100 ⫾ 1500 1200 ⫾ 175 13,100 ⫾ 1500 24,500 ⫾ 1750 9500 ⫾ 2000 18,000 ⫾ 1900 1500 ⫾ 300 5060 ⫾ 1000 6600 ⫾ 1500 8500 ⫾ 1400 9500 ⫾ 2100 3200 ⫾ 1200 850 ⫾ 180 18,200 ⫾ 2200 2100 ⫾ 230 18,000 ⫾ 2500 1330 ⫾ 250 7100 ⫾ 2000 13,000 ⫾ 3500 21,000 ⫾ 3100 750 ⫾ 150 320 ⫾ 100 420 ⫾ 120 600 ⫾ 200 N.D., not detectable. a Specific activity at 0.5 mM substrate concentration. conjugates and 15 selenocysteine Se-conjugates as substrates are shown in Table 1. From these results, it appears that most cysteine S-conjugates tested showed only a very low -elimination activity. Significant consumption of KMB was observed, indicative for a preference for the transamination reaction. The only L-cysteine S-conjugates showing a sufficiently high -elimination activity enabling assessment of enzyme kinetic parameters were the four cysteine S-conjugates carrying halogenated alkyl and alkenyl substituents. A decrease in specific activity and kcat/Km was observed in the order TFE-Cys ⬎ CTFE-Cys ⬎ DCDFE-Cys ⬇ 1,2-DCV-Cys. These nephrotoxic cysteine S-conjugates also appear to be transaminated to a significant extent as indicated by a significant consumption of KMB (Table 1). The relative importance of transamination appears to slightly increase in the order TFE-Cys ⬍ CTFE-Cys ⬍ DCDFE-Cys ⬇ 1,2-DCV-Cys. Compared with their sulfur analogs, the selenocysteine Se-conjugates appeared to be metabolized at much higher activities by purified cysteine conjugate -lyase/GTK (Table 1). The -elimination activities of several selenocysteine Seconjugates were almost equivalent to that of TFE-Cys, the best -elimination substrate for this enzyme known as yet. Furthermore, transamination appears to be an important pathway of metabolism of selenocysteine Se-conjugates, because KMB consumption was always significantly higher than pyruvic acid production. Of three selenocysteine Seconjugates, the D-selenocysteine Se-conjugates were also tested: Se-(methyl)-D-selenocysteine, Se-(n-propyl)-D-selenocysteine, and Se-(4-methylbenzyl)-D-selenocysteine. For these stereoisomers, however, no significant -elimination or KMB consumption was observed, indicative of absolute stereoselectivity of -lyase/GTK for the L-isomers. The pyruvic acid formation and KMB consumption of all substrates shown in Table 1 could be blocked completely by 1 mM amino-oxyacetic acid. The specific activities of -elimination of selenocysteine conjugates with n-alkyl-substituents were 30 to 50 times higher than that of the corresponding cysteine S-conjugates, enabling Lineweaver-Burke analyses. An increase in the length of the n-alkyl-chain appears to increase the affinity for -lyase/GTK, because the Michaelis-Menten constant Km decreases significantly from 5 mM (methyl substituent) to 0.26 mM (n-butyl-substituent). In contrast, kcat values decrease with increased chain length. Se-(n-Butyl)-L-selenocysteine showed substrate inhibition at concentrations higher than 0.5 mM. Therefore, the enzyme kinetic parameters for this substrate were estimated from the activities obtained below 0.5 mM. Se-Allyl-L-selenocysteine and S-allyl-L-cysteine, compounds known to occur in garlic (Lu et al., 1996), both showed -elimination on incubation with purified -lyase/GTK (Table 1). Again, the selenium compound showed a 30-fold higher specific activity at a concentration of 0.5 mM than its sulfur analog. For S-allyl-L-cysteine, enzyme activities were too low to allow determination of enzyme kinetic parameters Km and kcat. With Se-allyl-L-selenocysteine as substrate, KMB consumption was almost 4-fold higher than pyruvic acid formation, again indicative for extensive concurrent transamination. KMB consumption was also observed in incubations with S-allyl-L-cysteine although at an approximately 10-fold lower rate compared with its selenium analog. Selenocysteine Se-conjugates with benzyl substituents also showed high -elimination activities (Table 1). Interestingly, para-substitution at the benzyl group strongly increased en- Downloaded from jpet.aspetjournals.org at ASPET Journals on August 3, 2017 4-Methylbenzyl Transamination Reaction X 758 Commandeur et al. the initial 0.2 mM KMB was consumed during incubation. As was the case with Se-phenyl-L-selenocysteine and Se-(4methylbenzyl)-L-selenocysteine, pyruvic acid formation in fraction 10 was almost 2-fold higher at a 0.5 mM KMB concentration, resulting in an activity profile comparable with that of CTFE-Cys. To localize the protein -lyase/GTK in the FPLC fractions, Western blotting was performed using serum of rabbits immunized with the purified rat -lyase/GTK. Rabbit antiserum raised against highly purified rat renal -lyase/GTK was kindly provided by Dr. A. Yamauchi (Kobe University, Japan). A very strong cross-reactivity, with a mass identical with to of the purified -lyase/GTK, was observed in fraction 10. Of all other fractions, only a weak staining was observed in fraction 11 (data not shown). Fractions 12 to 18, which showed significant -elimination activity and KMB consumption, apparently did not contain the -lyase/GTK protein at all. Activity of aspartate aminotransferase was present only in fractions 8 and 9, with a 5-fold higher activity in fraction 8 (data not shown). The fact that fraction 8 does not show -elimination activity indicates that aspartate aminotransferase is not involved in the -elimination activity of the conjugates studied. Fractionation of Rat Kidney Cytosol by Gel Filtration Chromatography. Rat renal cytosol was also fractionated by high-resolution gel filtration chromatography using a HiLoad 16/60 Superdex 200 column. By using Se-(4-methylbenzyl)-L-selenocysteine and CTFE-Cys as substrates, activity profiles were determined. As shown in Fig. 4, at least two broad peaks with -elimination activity were shown. For both substrates, maximal activity was present in fraction 20, which eluted 150 min after the application of renal cytosol to the column. According to the calibration by the marker protein mixture, the mass of this protein will be around Mr 300,000. The second peak had its highest activity in fraction 37, which eluted after 167 min. According to the calibration by the marker protein mixture, the mass of this protein will between Mr 66,000 and 132,000, which may be consistent with the 90-kDa -lyase protein or proteins. Discussion Local activation of cysteine S-conjugates by renal -eliminating enzymes has been proposed as a novel approach to target antitumor compounds 6-mercaptopurine and 6-thioguanine to the kidney (Hwang and Elfarra, 1989, 1991). More recently, selenocysteine Se-conjugates were proposed as alternative kidney-selective prodrugs showing much higher -elimination activities in rat renal cytosol than their corresponding sulfur analogs (Andreadou et al., 1996). However, as yet little is known regarding which proteins are actually involved in the -elimination reactions of these S- and Seconjugates because activities have been measured only in crude enzyme fractions such as kidney homogenates, mitochondria, and cytosols. One of the enzymes capable of catalyzing transamination and -elimination reactions is -lyase/GTK. It has been suggested that large noncharged amino acids are transaminated by -lyase/GTK (Cooper and Meister, 1974). Using purified -lyase/GTK from rat kidney, next to transamination, a high -elimination activity has been demonstrated with 1,2-DCVCys and TFE-Cys as substrates. No -elimination activity Downloaded from jpet.aspetjournals.org at ASPET Journals on August 3, 2017 zyme activities compared with the unsubstituted Se-benzylL-selenocysteine; -elimination activities almost equaled those of TFE-Cys. Because KMB consumption even exceeds pyruvic acid formation, these results suggest that overall activity (i.e., -elimination plus transamination) of the substituted Se-benzyl-L-selenocysteines is even higher than that of TFE-Cys. No clear differences were observed between electron-withdrawing (chloro substituents) and electron-donating (methyl and methoxy substituents) substituents, suggesting that steric or lipophilic effects are more important than electronical effects. Se-phenyl-L-selenocysteine also is a very good substrate for -lyase/GTK, as indicated by its high activities of pyruvic acid production and KMB consumption. The specific activity of Se-phenyl-L-selenocysteine at 0.5 mM is more than 150fold higher than that of S-phenyl-L-cysteine (Table 1), again pointing to the superiority of selenocysteine Se-conjugates over cysteine S-conjugates as substrates for -lyase/GTK. However, in contrast to the benzyl-substituted selenocysteine-Se-conjugates, the introduction of para-substituents at the phenyl ring of Se-phenyl-L-selenocysteine results in a dramatic decrease in enzyme activity (Table 1). To generate sufficient pyruvic acid, incubation with these substrates were performed for 20 min and in presence of a 10-fold higher concentration of purified -lyase/GTK. Se-(4-methylphenyl)L-selenocysteine and Se-(4-chlorophenyl)-L-selenocysteine demonstrated substrate inhibition at concentrations higher than 0.5 mM. Se-(4-methoxyphenyl)-L-selenocysteine was the selenocysteine Se-conjugates displaying the lowest pyruvic acid formation (Table 1). Fractionation of Rat Kidney Cytosol by Anion Exchange Chromatography. Rat renal cytosol was fractionated using Mono-Q anion exchange FPLC, and the 2-ml fractions collected were subsequently screened for -lyase activity using five different substrates. Using CTFE-Cys as a substrate, the highest activity was found in fraction 10, whereas lower activities were found in fractions 11, 12, and 13 (Fig. 3A). In incubations of fractions with CTFE-Cys as a substrate, only a relatively small consumption of KMB was observed (⬍20% of the initial 0.2 mM concentration). When using Se-phenyl-L-selenocysteine and Se-(4-methylbenzyl)-Lselenocysteine as substrate, however, a significantly different activity profile was observed, which rules out involvement of only a single enzyme in the -elimination reactions. First, in contrast to CTFE-Cys, maximal activity was observed in fraction 11 (Fig. 3, B and C). Second, using these selenocysteine conjugates, very significant pyruvic acid formation was also observed in fractions 14 to 18. Furthermore, with both substrates, a much stronger, up to 60%, consumption of KMB was observed, which again was significant from fractions 10 to 18. To test whether KMB might have become limiting in these experiments, the activity of these fractions were also determined in presence of 0.5 mM KMB. Interestingly, at this higher KMB concentration, the highest pyruvate formation was observed in fraction 10, as is the case with CTFE-Cys as substrate (data not shown). Se-(isopropyl)-L-selenocysteine (Fig. 3D) and Se-allyl-L-selenocysteine (data not shown), at 0.2 mM KMB, had almost identical activity profiles with maximal activity in fractions 10, 11, and 12 and low but still significant activities in fractions 13 to 18. With these selenocysteine Se-conjugates, KMB consumption was even more significant; up to 80% of Vol. 294 2000 Bioactivation of Se-Conjugates by -Lyase/GTK 759 was observed using S-(benzothiazolyl)-L-cysteine (BTC) (Yamauchi et al., 1993), 5⬘-S-cysteinyldopamine and leukotriene E4 as substrates (Abraham et al., 1995). In the present study, nine additional cysteine S-conjugates were evaluated as substrates for purified renal -lyase/GTK. As shown in Table 1, high -elimination activities were also observed with CTFE-Cys and DCDFE-Cys as substrate. In accordance with the observations by Stevens et al. (1986) using 1,2-DCV-Cys, significant transaminase activity was also observed as implicated by the relatively high rates of KMB consumption that were observed. The present study confirms that cysteine S-conjugates carrying nonhalogenated substituents are poor substrates in comparison with their selenium analogs (Table 1). Enzymatic pyruvic acid production was only 25 to 50% of the low nonenzymatic degradation of the conjugates (data not shown). Only at a higher enzyme concentration and with a longer incubation time was significant consumption of KMB observed for these S-conjugates, indicative of a preference for transamination reactions. Although the present nonhalogenated cysteine S-conjugates have not yet been tested with the purified rat -lyase/GTK, some of them have been tested previously as substrates for purified -lyases from bovine and turkey kidney (Bhattacharya and Schultze, 1967). Consistent with the present study, no -elimination was previously observed with S-methyl-L-cysteine, S-ethyl-L-cysteine, S-propyl-L-cysteine, S-benzyl-L-cysteine, and S-allyl-L-cysteine. Lash et al. (1990) reported that BTC and S-(benzothiazolyl)-L-homocysteine were actively -eliminated by a purified -lyase from human kidney. However, Yamauchi et al. (1993) did not find any activity of purified rat renal -lyase/ GTK toward BTC, suggesting a significant species difference Downloaded from jpet.aspetjournals.org at ASPET Journals on August 3, 2017 Fig. 3. Activity profiles of -elimination reactions (indicated by pyruvate formation) and transamination reactions (as indicated by KMB consumption) with CTFE-Cys and three selenocysteine Se-conjugates in FPLC fractions from rat renal cytosol applied to a Mono-Q anion exchange column. Activities were expressed as percentage of the highest peak area of pyruvate and KMB, respectively. 760 Commandeur et al. in substrate selectivity between the human and rat enzymes. This species difference is further supported by the overall 18% dissimilarity between the amino acid sequences of the rat and human enzyme (Perry et al., 1993). Selenocysteine Se-conjugates previously were shown to be -eliminated at a high activity by renal cytosol (Andreadou et al., 1996). The results of the present study suggest that -lyase/GTK plays a major role in this reaction, because most Se-conjugates were -eliminated at a very high activity. Several selenocysteine Se-conjugates displayed -elimination activities as high as that observed with TFE-Cys, which was the best substrate known. As was observed previously in rat renal cytosol, the activity of -elimination of Se-conjugates was much higher than -elimination of corresponding Sconjugates (Table 1). Possible explanations for the higher -elimination reactions of selenocysteine Se-conjugates may be the weaker bond strength of the C-Se-bond (234 kJ/mol) versus C-S-bonds (272 kJ/mol) (Guziec, 1987) and/or a more facilitated -proton abstraction of the selenocysteine moiety (Miles, 1986). As indicated by the significant KMB consumption, transamination reaction is a prominent pathway of biotransformation of both selenocysteine Se-conjugates and cysteine S-conjugates. When comparing specific activities of transamination, selenocysteine Se-conjugates as substrates showed 5- to 10-fold higher activities compared with their sulfur analogs (Table 1). Because transamination reactions do not involve C-Se-scission, facilitation of -proton abstraction by electronic effects of the Se atom may be the most likely explanation. Because only very few substrates have been identified as yet, little was known regarding the structure-activity relationship of -lyase/GTK-catalyzed -elimination reactions. Because of their surprisingly high activities, the class of selenocysteine Se-conjugates may be interesting probe substrates to characterize the substrate-binding site. For the alkyl-substituted Se-conjugates, an increase in the alkylchain appears to increase the affinity for the enzyme, as indicated by the decrease in their Km value (Table 1). The benzyl-substituted Se-conjugates appeared to be extremely good substrates as well, especially when substituted at the para-position of the benzyl group. No clear electronic effects were observed because both electron-withdrawing and electron-donating para-substituents appeared to increase -elimination activity compared with Se-benzyl-L-selenocysteine. Therefore, apparently steric properties or lipophilicity plays a more important role. Surprisingly, the introduction of para-substituents in Se-phenyl-L-selenocysteine led to a very strong decrease in enzyme activities (Table 1). When comparing enzyme kinetic parameters of the purified -lyase/GTK with those obtained previously with rat renal cytosol, a very poor correlation is found. The most striking difference is found with the para-substituted Sephenyl-L-selenocysteine compounds, which showed high activity with rat renal cytosol (Andreadou et al., 1996) but demonstrated only a minor activity with purified -lyase/ GTK. By determining the activity profile in fractionated rat renal cytosol, the present study reveals that the poor correlation between cytosolic and purified enzyme activities may be explained by the involvement of multiple enzymes in the cytosolic fractions (Figs. 3 and 4). Aspartate aminotransferase, which was proposed by Kato et al. (1996) as an alternative rat renal -lyase enzyme, does not appear to be involved in -elimination of the conjugates tested. According to the results of the gel filtration chromatography, an important role may be played by the high-molecular-weight -lyase previously characterized by Abraham et al. (1995). Recently, it was shown that selenium-enriched garlic was more effective in cancer prevention than normally grown garlic (Lu et al., 1996). Among the selenium species identified in selenium-enriched garlic were two of the selenocysteine Se-conjugates, Se-methyl-L-selenocysteine and Se-allyl-L-selenocysteine, tested in this study. Dietary administration of these selenocysteine Se-conjugates to rats indeed provided strong protection against methylnitrosourea-induced carcinogenesis in rats (Ip et al., 1999). Chemopreventive activities of these selenocysteine Se-conjugates have been attributed to methyl selenol (Ip and Ganther, 1992) and diallyl selenide (Lu et al., 1996), respectively, both of which are formed via -elimination reactions. The relatively high activity of purified -lyase/GTK toward these selenocysteine Se-conjugates (Table 1) may implicate a role of -lyase/GTK in the chemopreventive activity of selenium-enriched garlic. Diallyl selenide was reported to have a 100-fold higher chemopreventive activity than diallyl sulfide, which is an important chemopreventive agent in normally grown garlic (el-Bayoumy et al., 1996). Therefore, the combination of higher bioactivation activity with formation of a 100-fold more potent product may explain the higher chemopreventive activity of Se-allyl-L-selenocysteine compared with S-allyl-L-cysteine. In conclusion, the results presented here indicate that rat renal -lyase/GTK plays an important role in the -elimination of selenocysteine Se-conjugates by rat renal cytosol. The high -elimination activity in combination with the potent antitumor activities of the formed selenol compounds makes selenocysteine Se-conjugates promising prodrugs to treat renal cell carcinoma. Fractionation of renal cytosol by two different types of chromatography, however, revealed that next to -lyase/GTK, additional enzymes are active in the -elimination of selenocysteine Se-conjugates. The identity and tissue distribution of these additional enzymes remain to Downloaded from jpet.aspetjournals.org at ASPET Journals on August 3, 2017 Fig. 4. Activity profiles of -elimination reactions (indicated by pyruvate formation) with CTFE-Cys (E) and Se-(4-methylbenzyl)-L-selenocysteine (F) in FPLC fractions from rat renal cytosol applied to a HiLoad 16/60 Superdex 200 gel filtration column. Vol. 294 2000 be established to predict the kidney selectivity of selenocysteine Se-conjugates as prodrugs. Acknowledgments Dr. S. Jespersen (TNO Nutrition and Food Research) at the Department of Bio-Pharmaceutical Analysis (Zeist, the Netherlands) is gratefully acknowledged for performing the MALDI-TOF mass spectrometry measurements on -lyase/GTK. References 761 Freeman F, Huang B-G and Lin RI-S (1994) Garlic chemistry: Nitric oxide oxidation of S-(2-propenyl)cysteine and (⫹)-S-(2-propenyl)-L-cysteine sulfoxide. J Org Chem 59:3227–3229. Guziec FS (1987) The Chemistry of Organic Selenium and Tellurium Compounds (Patai S and Rappaport Z eds) pp 215, Wiley and Sons, Chichester, UK. Hwang IY and Elfarra AA (1991) Kidney-selective prodrugs of 6-mercaptopurine: Biochemical basis of the kidney selectivity of S-(6-purinyl)-L-cysteine and metabolism of new analogs in rats. J Pharmacol Exp Ther 258:171–177. Hwang IY and Elfarra AA (1989) Cysteine S-conjugates may act as kidney-selective prodrugs: Formation of 6-mercaptopurine by the renal metabolism of S-(6purinyl)-L-cysteine. J Pharmacol Exp Ther 251:448 – 454. Ip C and Ganther HE (1992) Comparison of selenium and sulfur analogs in cancer prevention. Carcinogenesis 13:1167–1170. Ip C, Zhu Z, Thompson HJ, Lisk D and Ganther HE (1999) Chemoprevention of mammary cancer with Se-allylselenocysteine and other selenoamino acids in the rat. Anticancer Res 19:2875–2880. Jespersen S, Niessen WMA, Tjaden UR and van der Greef J (1995) Quantitative bioanalysis using matrix-assisted laser desorption mass spectrometry. J Mass Spectrom 30:357–364. Kato Y, Asano Y and Cooper AJL (1996) Inactivation of brain and kidney aspartate aminotransferases by S-(1,2-dichlorovinyl)-L-cysteine and by S-(1,1,2,2tetrafluoroethyl)-L-cysteine. Dev Neurosci 18:505–514. Lash LL, Nelson RM, Van Dyke RA and Anders MW (1990) Purification and characterization of human kidney cytosolic cysteine conjugate beta-lyase activity. Drug Metab Dispos 18:50 –54. Lu JX, Pei HY, Ip C, Lisk DJ, Ganther H and Thompson HJ (1996) Effect on an aqueous extract of selenium-enriched garlic on in vitro markers and in vivo efficacy in cancer prevention. Carcinogenesis 17:1903–1907. McKinney LL, Picken JC Jr, Weakley FB, Eldridge AC, Campbell RE, Cowan JC and Biester HE (1959) Possible toxic factor of trichloroethylene-extracted soybean oil meal. J Am Chem Soc 81:909 –915. Miles EW (1986) Coenzymes and cofactors, in Vitamin B6 Pyridoxal Phosphate: Chemical, Biochemical and Medical Aspects (Dolphin D, Poulson R and Avramovic O eds) vol 1, pp 253–310, John Wiley & Sons, New York. Mosca M, Cozzi L, Breton J, Speciale C, Okuno E, Schwarcz R and Benatti L (1994) Molecular cloning of rat kynurenine aminotransferase: Identity with glutamine transaminase K. FEBS Lett 353:21–24. Perry SJ, Schofield MA, MacFarlane M, Lock EA, King LJ, Gibson GG and Goldfarb PS (1993) Isolation and expression of a cDNA coding for rat kidney cytosolic cysteine conjugate beta-lyase. Mol Pharmacol 43:660 – 665. Stevens JL, Robbins JD and Byrd RA (1986) A purified cysteine conjugate beta-lyase from rat kidney cytosol. Requirement for an alpha-keto acid or an amino acid oxidase for activity and identity with soluble glutamine transaminase K. J Biol Chem 261:15529 –15537. Stijntjes GJ, Te Koppele JM and Vermeulen NPE (1992) High-performance liquid chromatography-fluorescence assay of pyruvic acid to determine cysteine conjugate beta-lyase activity: Application to S-1,2-dichlorovinyl-L-cysteine and S-2benzothiazolyl-L-cysteine. Anal Biochem 206:334 –343. Yamauchi A, Stijntjes GJ, Commandeur JNM and Vermeulen NPE (1993) Purification of glutamine transaminase K/cysteine conjugate beta-lyase from rat renal cytosol based on hydrophobic interaction HPLC and gel permeation FPLC. Protein Expr Purif 4:552–562. Send reprint requests to: Dr. Jan N. M. Commandeur, Leiden/Amsterdam Center for Drug Research (LACDR), Division of Molecular Toxicology, Department of Pharmacochemistry, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, the Netherlands. E-mail: [email protected] Downloaded from jpet.aspetjournals.org at ASPET Journals on August 3, 2017 Abraham DG and Cooper AJL (1996) Cloning and expression of a rat kidney cytosolic glutamine transaminase K that has strong sequence homology to kynurenine pyruvate aminotransferase. Arch Biochem Biophys 335:311–320. Abraham DG, Patel PP and Cooper AJL (1995) Isolation from rat kidney of a cytosolic high molecular weight cysteine-S-conjugate beta-lyase with activity toward leukotriene E4. J Biol Chem 270:180 –188. Abraham DG, Thomas RJ and Cooper AJL (1995) Glutamine transaminase K is not a major cysteine S-conjugate beta-lyase of rat kidney mitochondria: Evidence that a high-molecular weight enzyme fulfills this role. Mol Pharmacol 48:855– 860. Andreadou I, Menge WMPB, Commandeur JNM, Worthington EA and Vermeulen NPE (1996) Synthesis of novel Se-substituted selenocysteine derivatives as potential kidney selective prodrugs of biologically active selenol compounds: Evaluation of kinetics of beta-elimination reactions in rat renal cytosol. J Med Chem 39:2040 – 2046. Bhattacharya RK and Schultze MO (1967) Enzymes from bovine and turkey kidneys which cleave S-(1,2-dichlorovinyl)-L-cysteine. Comp Biochem Physiol 22:723–735. Commandeur JNM, Brakenhoff JPG, de Kanter FJJ and Vermeulen NPE (1988) Nephrotoxicity of mercapturic acids of three structurally related 2,2-difluoroethylenes in the rat: Indications for different bioactivation mechanisms. Biochem Pharmacol 37:4495– 4505. Commandeur JNM, King LJ, Koymans L and Vermeulen NPE (1996) Bioactivation of S-(2,2-dihalo-1,1-difluoroethyl)-L-cysteines and S-(trihalovinyl)-L-cysteines by cysteine S-conjugate beta-lyase: Indications for formation of both thionoacylating species and thiiranes as reactive intermediates. Chem Res Toxicol 9:1092–1102. Commandeur JNM, Stijntjes GJ and Vermeulen NPE (1995) Enzymes and transport systems involved in the formation and disposition of glutathione S-conjugates: Role in bioactivation and detoxication mechanisms of xenobiotics. Pharmacol Rev 47:271–330. Cooper AJL (1998) Mechanisms of cysteine S-conjugate beta-lyases. Adv Enzymol 72:199 –238. Cooper AJL and Meister A (1974) Isolation and properties of a new glutamine transaminase from rat kidney. J Biol Chem 249:2554 –2561. Dekant W, Berthold K, Vamvakas S, Henschler D and Anders MW (1988) Thioacylating intermediates as metabolites of S-(1,2-dichlorovinyl)-L-cysteine and S-(1,2,2-trichlorovinyl)-L-cysteine formed by cysteine conjugate beta-lyase. Chem Res Toxicol 1:175–178. el-Bayoumy K, Chae YH, Upadhyaya P and Ip C (1996) Chemoprevention of mammary cancer by diallyl selenide, a novel organoselenium compound. Anticancer Res 16:2911–2915. Elfarra AA, Duescher RJ, Hwang IY, Sicuri AR and Nelson JA (1995) Targeting 6-thioguanine to the kidney with S-(guanin-6-yl)-L-cysteine. J Pharmacol Exp Ther 274:1298 –1304. Elfarra AA and Hwang IY (1993) Targeting of 6-mercaptopurine to the kidneys: Metabolism and kidney-selectivity of S-(6-purinyl)-L-cysteine analogs in rats. Drug Metab Dispos 21:841– 845. Bioactivation of Se-Conjugates by -Lyase/GTK