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THE PROTEOME RESPONSE OF LARVAL STAGES OF Crassostrea hongkongensis TO LONG TERM EXPOSURE TO HIGH-CO 2 R Dineshram, V Thiyagarajan The Swire Institute of Marine Science, The University of Hong Kong, Cape d'Aguilar, Shek O, Hong Kong, Email: [email protected] Oysters are one of the most important commercially exploited species cultured in the molluskan hatcheries around the world. Due to rising CO 2 and subsequent decrease in seawater pH, their survival and shell forming processes are threatened globally. Our large-scale CO 2 perturbation experiments at a commercial hatchery setting showed that larval shell growth rate is significantly reduced at projected OA scenarios for the year >2100 in the oyster species (Crassostrea hongkongensis) compared to that of ambient CO 2 levels. 2DE-MALDI TOF/TOF based proteomics approach was used to examine the global (total) and the post translational modified proteins of oyster larvae exposed to OA over 32 d. Proteomic analysis of the eye-spot larvae of statistically similar size at the time of settlement, displayed protein expression pattern and/or phosphorylation levels decreased with increasing OA stress. The differential expressed proteins that are identified in this study are related to energy metabolism, calcium binding and cytoskeletal proteins. Disruption of cytoskeletal and calcification responsible proteins observed due to OA in the early stages of the edible oyster can be estimated to have a significant implication with its population structure and in its commercial production in particular to aquaculture. This study on early larval stages coupled with proteome change in OA conditions is the first step towards the search for novel protein expression signatures that can provide new insights into their role in tolerance and/or as biomarker for OA.