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III. - Sugars and Polysaccharides Gérald Monard Structure et réactivité des biopolymères (Biopolymer structure and reactivity) Master Chimie 2ème année 1 Contents 1 Monosaccharides 1.1 Classification . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Configurations and Conformations . . . . . . . . . . . . . . . 1.3 Sugar derivatives . . . . . . . . . . . . . . . . . . . . . . . . 5 5 8 14 2 Polysaccharides 2.1 Disaccharides . . . . . . . . . . . . . . . . . . . . . . . . . . 2.2 Cellulose and chitin . . . . . . . . . . . . . . . . . . . . . . . 2.3 Starch and Glycogen . . . . . . . . . . . . . . . . . . . . . . 16 17 21 25 3 Glycoproteins 3.1 Bacterial Cell Walls . . . . . . . . . . . . . . . . . . . . . . . 3.2 Glycoprotein Structure . . . . . . . . . . . . . . . . . . . . . 30 31 38 2 Carbohydrates or saccharides are the most abundant biological molecules. They are chemically simpler than nucleotides or amino acids, containing just three elements (C, O, H) combined according to the formula (C.H2 O)n where n ≥ 3. The basic carbohydrate units are called monosaccharides. They differ in their number of carbon atoms and in the arrangement of the H and O atoms attached to the carbons. Many of these compounds are synthesized from simpler substances in a process named gluconeogenesis. Others are the products of photosynthesis. The metabolic breakdown of monosaccharides provides much of the energy used to power biological processes. Monosaccharides are also principal components of nucleic acids, as well as important elements of complex lipids. 3 Oligosaccharides consist of a few covalently linked monosaccharide units. They are often associated with proteins (glycoproteins) and lipids (glycolipids) in which they have both structural and regulatory functions (glycoproteins and glycolipids are collectively called glycoconjugates). Polysaccharides consist of many covalently linked monosaccharide units and have molecular masses ranging well into the millions of daltons. They have indispensable structural functions in all types of organisms but are most conspicuous in plants because cellulose, their principal structural material, comprises up to 80% of their dry weight. Polysaccharides such as starch in plants and glycogen in animals serve as important nutritional reservoirs. 4 1 Monosaccharides Monosaccharides are aldehyde or ketone derivatives of straight-chain polyhydroxy alcohols containing at least three carbon atoms. 1.1 Classification Monosaccharides are classified according to the chemical nature of their carbonyl group and the number of their C atoms. If the carbonyl group is an aldehyde, the sugar is an aldose. If the carbonyl group is a ketone, the sugar is a ketose. The smallest monosaccharides, those with three carbon atoms, are trioses. Those with four, five, six, seven, etc. C atoms are, respectively, tetroses, pentoses, hexoses, heptoses, etc. These terms may be combined so that, for example, glucose is an aldohexose, whereas ribulose is a ketopentose. 5 Figure 1: The stereochemical relationships, shown in Fischer projection, among the D-aldoses with three to six carbon atoms. 6 Figure 2: The stereochemical relationships among the D-ketoses with three to six carbon atoms. 7 1.2 Configurations and Conformations Alcohols react with the carbonyl groups of aldehydes and ketones to form hemiacetals and hemiketals, respectively. Figure 3: The reactions of alcohols with (a) aldehydes to form hemiacetals and (b) ketones to form hemiketals. 8 The hydroxyl and either the aldehyde or the ketone functions of monosaccharides can likewise react intramolecularly to form cyclic hemiacetals and hemiketals. The configurations of the substituents to each carbon atom of these sugar rings are conveniently represented by their Haworth projection formulas. A sugar with a six-membered ring is known as a pyranose in analogy with pyran, the simplest compound containing such a ring. Similarly, sugars with five-membered rings are designated furanoses in analogy with furan. 9 Figure 4: Cyclization reactions for hexoses. (a) D-Glucose in its linear form reacting to yield the cyclic hemiacetal α-D-glucopyranose and (b) D-fructose in its linear form reacting to yield the hemiketal α-D-fructofuranose. 10 When a monosaccharide cyclizes, the carbonyl carbon, called the anomeric carbon, becomes a chiral center with two possible configurations. The pair of stereoisomers that differ in configuration at the anomeric carbon are called anomers. In the α anomer, the OH substituent of the anomeric carbon is on the opposite side of the sugar ring from the CH2OH group at the chiral center that designates the D or L configuration. The other form is known as the β anomer. Figure 5: α and β anomers . 11 The pyranose ring, like the cyclohexane ring, may assume a boat or a chair conformation. Figure 6: Conformations of the cyclohexane ring. The relative stabilities of these various conformations depend on the stereochemical interactions between the substituents on the ring. The boat conformer crowds the substituents on its “bow” and “stern” and eclipses those along its sides, so that in cyclohexane it is ∼25 kJ/mol less stable than the chair conformer. 12 The ring substituents on the chair conformer fall into two geometrical classes: the axial groups and the equatorial groups. Figure 7: The two alternative chair conformations of β -D-glucopyranose. Since the axial and equatorial groups on a cyclohexane ring are conformationally interconvertible, a given ring has two alternative chair forms; the one that predominates usually has the lesser crowding among its axial substituents. The conformational situation of a group directly affects its chemical reactivity. For example, equatorial OH groups on pyranoses esterify more readily than do axial OH groups. 13 1.3 Sugar derivatives The chemistry of monosaccharides is largely that of their hydroxy and carbonyl groups. For example, in an acid-catalyzed reaction, the anomeric hydroxyl of a sugar reversibly condenses with alcohols to form α- and β -glycosides. The bond connecting the anomeric carbon to the acetal oxygen is termed a glycosidic bond. Figure 8: The acid-catalyzed condensation of α-D-glucose with methanol to form an anomeric pair of methyl-D-glucosides. 14 Polysaccharides are held together by glycosidic bonds between neighboring monosaccharide units. The glycosidic bond is therefore the carbohydrate analog of the peptide bond in proteins. The bond in a nucleoside linking its ribose residue to its base is also a glycosidic bond. The hydrolysis of glycosidic bonds is catalyzed by enzymes known as glycosidases. 15 2 Polysaccharides Polysaccharides, which are also known as glycans, consist of monosaccharides linked together by glycosidic bonds. They are classified as homopolysaccharides or heteropolysaccharides if they consist of one type or more than one type of mono saccharide. Homopolysaccharides may be further classified according to the identity of their monomeric unit. For example, glucans are polymers of glucose, whereas galactans are polymers of galactose. Although the monosaccharide sequences of heteropolysaccharides can, in principle, be even more varied than those of proteins, many are composed of only a few types of monosaccharides that alternate in a repetitive sequence. Polysaccharides, in contrast to proteins and nucleic acids, form branched as well as linear polymers. This is because glycosidic linkages can be made to any of the hydroxyl groups of a monosaccharide. Fortunately for structural biochemists, most polysaccharides are linear and those that branch do so in 16 only a few well-defined ways. A complete description of an oligosaccharide or polysaccharide includes the identities, anomeric forms, and linkages of all its component monosaccharide units. 2.1 Disaccharides Oligosaccharides containing three or more residues are relatively rare, occur ring almost entirely in plants. Disaccharides, the simplest polysaccharides, are more common. Many occur as the hydrolysis products of larger molecules. 17 Sucrose, the most abundant disaccharide, occurs throughout the plant kingdom and is familiar to us as common table sugar. To name a polysaccharide systematically, one must specify its component monosaccharides, their ring types, their anomeric forms, and how they are linked together. Sucrose is therefore O-α-D-glucopyranosyl-(1→2)-β -D-fructofuranoside, where the symbol (1→2) indicates that the glycosidic bond links C1 of the glucose residue to C2 of the fructose residue. 18 Lactose [O-β -D-galactopyranosyl-(1→4)-D-glucopyra nose] or milk sugar occurs naturally only in milk, where its concentration ranges from 0 to 7% depending on the species. 19 There are several common glucosyl-glucose disaccharides. These include maltose [O-α-D-glucopyranosyl-(1→4)-D-glucopyranose], an enzymatic hydrolysis product of starch; isomaltose, its α(1→6) isomer; and cellobiose, its β (1→4) isomer, the repeating disaccharide of cellulose. 20 2.2 Cellulose and chitin Plants have rigid cell walls that can withstand osmotic pressure differences between the extracellular and intracellular spaces of up to 20 atm. Cellulose, the primary structural component of plant cell walls, accounts for over half of the carbon in the biosphere: Approximately 1015 kg of cellulose is estimated to be synthesized and degraded annually. Cellulose is a linear polymer of up to 15,000 D-glucose residues linked by β (1→4) glycosidic bonds. Figure 9: The primary structure of cellulose (here n may be several thousand). 21 X-Ray and other studies of cellulose fibers reveal that cellulose chains are flat ribbons in which successive glucose rings are turned over 180° with respect to each other. This permits the C3-OH group of each glucose residue to form a hydrogen bond with the ring oxygen (O5) of the next residue. Figure 10: Proposed structural model of cellulose 22 Parallel cellulose chains form sheets with interchain hydrogen bonds, including O2-H· · · O6 and O6-H· · · O3 bonds. Stacks of these sheets are held together by hydrogen bonds and van der Waals interactions. This highly cohesive structure gives cellulose fibers exceptional strength and makes them water insoluble despite their hydrophilicity. In plant cell walls, the cellulose fibers are embedded in and cross-linked by a matrix containing other polysaccharides and lignin, a plastic-like phenolic polymer. The resulting composite material can withstand large stresses because the matrix evenly distributes the stresses among the cellulose reinforcing elements. 23 Chitin is the principal structural component of the exoskeletons of invertebrates such as crustaceans, insects, and spiders and is also a major cell wall constituent of most fungi and many algae. It is estimated that ∼ 1014 kg of chitin are produced annually, most of it in the oceans, and therefore that it is almost as abundant as is cellulose. Chitin is a homopolymer of β (1→4)-linked N-acetyl-D-glucosamine residue. It differs chemically from cellulose only in that each C2-OH group is replaced by an acetamido function. X-ray analysis indicates that chitin and cellulose have similar structures. 24 2.3 Starch and Glycogen Starch is a mixture of glycans that plants synthesize as their principal energy reserve. It is deposited in the chloroplasts of plant cells as insoluble granules composed of α-amylose and amylopectin. α-Amylose is a linear polymer of several thousand glucose residues linked by α(1→4) bonds. 25 Note that although α-amylose is an isomer of cellulose, it has very different structural properties. While cellulose’s β -glycosidic linkages cause it to assume a tightly packed, fully extended conformation , α-amylose’s α-glycosidic bonds cause it to adopt an irregularly aggregating helically coiled conformation. 26 Amylopectin consists mainly of α(1→4)-linked glucose residues but is a branched molecule with α(1→6) branch points every 24 to 30 glucose residues on average. Amylopectin molecules contain up to 106 glucose residues, which makes them among the largest molecules occurring in nature. Figure 11: Amylopectin. (left) Its primary structure near one of its α(1→6) branch (red). (right) Its bushlike structure with glucose residues at branch points averages 24 to 30 glucose residues. 27 The digestion of starch, the main carbohydrate source in the human diet, occurs in stages. It begins in the mouth. Saliva contains an amylase, which randomly hydrolyzes the α(1→4) glycosidic bonds of starch. Starch digestion continues in the small intestine under the influence of pancreatic amylase, which degrades starch to a mixture of small oligosaccharides. Further hydrolysis by an α-glucosidase, which removes one glucose residue at a time, and by a debranching enzyme, which hydrolyzes α(1→6) as well as α(1→4) bonds, produces monosaccharides that are absorbed by the intestine and transported to the bloodstream. 28 Glycogen, the storage polysaccharide of animals, is present in all cells but is most prevalent in skeletal muscle and in liver, where it occurs as cytoplasmic granules. The primary structure of glycogen resembles that of amylopectin, but glycogen is more highly branched, with branch points occurring every 8 to 14 glucose residues. In the cell, glycogen is degraded for metabolic use by glycogen phosphorylase, which phosphorolytically cleaves glycogen’s α(1→4) bonds sequentially inward from its nonreducing ends. Glycogen’s highly branched structure, which has many nonreducing ends, permits the rapid mobilization of glucose in times of metabolic need. The α(1→6) branches of glycogen are cleaved by glycogen debranching enzyme. 29 3 Glycoproteins Many proteins are actually glycoproteins, with carbohydrate contents varying from <1% to > 90% by weight. Glycoproteins occur in all forms of life and have functions that span the entire spectrum of protein activities, including those of enzymes, transport proteins, receptors, hormones, and structural proteins. The polypeptide chains of glycoproteins, like those of all proteins, are synthesized under genetic control. Their carbohydrate chains, in contrast, are enzymatically generated and covalently linked to the polypeptide without the rigid guidance of nucleic acid templates. For this reason, glycoproteins tend to have variable carbohydrate composition, a phenomenon known as microheterogeneity. Characterizing the structures of carbohydrates –and their variations– is one goal of the field of glycomics, which complements the studies of genomics (for DNA) and proteomics (for proteins). 30 3.1 Bacterial Cell Walls Bacteria are surrounded by rigid cell walls that give them their characteristic shapes and permit them to live in hypotonic (less than intracellular salt con centration) environments that would otherwise cause them to swell osmotically until their plasma (cell) membranes lysed (burst). Bacterial cell walls are of considerable med ical significance because they are responsible for bacterial virulence (disease-evoking power). In fact, the symptoms of many bacterial diseases can be elicited in animals merely by the injection of bacterial cell walls. Furthermore, the characteristic antigens (immunological markers) of bacteria are components of their cell walls and capsules, so that injection of preparations of these sub- stances into an animal often invokes its immunity against these bacteria. 31 Bacteria are classified as gram-positive or gram-negative depending on whether or not they take up gram stain. 1 Gram-positive bacteria have a thick (∼250 Å) cell wall surrounding their plasma membrane, whereas gram-negative bacteria have a thin (∼30 Å) cell wall covered by a complex outer membrane. Figure 12: Schematic diagram comparing the cell envelopes of (a) gram-positive bacteria and (b) gram-negative bacteria. 1a procedure developed in 1884 by Christian Gram in which heat-fixed cells are successively treated with the dye crystal violet and iodine and then destained with either ethanol or acetone. Gram-negative bacteria possess a complex outer membrane that surrounds their cell wall and excludes gram stain, whereas grampositive bacteria lack such a membrane. 32 The cell walls of both gram-positive and gram-negative bacteria consist of covalently linked polysaccharide and polypeptide chains that form a framework that completely encases the cell. This substance is known as a peptidoglycan. Its polysaccharide component consists of linear chains of alternating β (1→4)linked N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM). The NAM’s lactic acid residue forms an amide bond with a D-amino acidcontaining tetrapeptide to form the peptidoglycan repeating unit. Neighboring parallel peptidoglycan chains are covalently cross-linked through their tetrapeptide side chains. In the gram-positive bacterium Staphylococcus aureus, whose tetrapeptide has the sequence L-Ala-D-isoglutamyl-L-Lys-D-Ala, this cross-link consists of a pentaglycine chain that extends from the terminal carboxyl group of one tetrapeptide to the ε-amino group of the Lys in a neighboring tetrapeptide. 33 Figure 13: Chemical structure of peptidoglycan. The repeating unit of peptidoglycan is an NAG-NAM disaccharide whose lactyl side chain forms an amide bond with a tetrapeptide. The tetrapeptide of S. aureus is shown. 34 Figure 14: Chemical structure of peptidoglycan. The S. aureus bacterial cell wall peptidoglycan. In other gram-positive bacteria, the Gly5 connecting bridges shown here may contain different amino acid residues such as Ala or Ser. In gram-negative bacteria, the peptide chains are directly linked via peptide bonds. 35 The D-amino acids of peptidoglycans render them resistant to proteases. In 1928, Fleming noticed that the chance contamination of a bacterial culture plate with the mold Penicillium notatum lysed nearby bacteria. This was caused by the presence of penicillin, an antibiotic secreted by the mold. Figure 15: Structure of penicillin Penicillin contains a thiazolidine ring (red) fused to a β lactam ring (blue). A variable R group is bonded to the β -lactam ring via a peptide linkage. 36 Penicillin specifically binds to and inactivates enzymes that function to crosslink the peptidoglycan strands of bacterial cell walls. Since cell wall expansion also requires the action of enzymes that degrade cell walls, exposure of growing bacteria to penicillin results in their lysis; that is, penicillin disrupts the normal balance between cell wall biosynthesis and degradation. However, since no human enzyme binds penicillin, it is of low human toxicity, a therapeutic necessity. Most bacteria that are resistant to penicillin secrete a β -lactamase (also known as penicillinase), which inactivates penicillin by hydrolytically cleaving the amide bond of its β -lactam ring. 37 3.2 Glycoprotein Structure Almost all the secreted and membrane-associated proteins of eukaryotic cells are glycosylated. Oligosaccharides are covalently attached to proteins by either N-glycosidic or O-glycosidic bonds. In N-linked oligosaccharides, NAG is invariably β -linked to the amide nitrogen of an Asn residue in the sequence Asn-X-Ser or Asn-X-Thr, where X is any amino acid except Pro and only rarely Asp, Glu, Leu, or Trp. 38 N-Glycosylation occurs cotranslationally, that is, while the polypeptide is being synthesized. Proteins containing N-linked oligosaccharides typically are glycosylated and then processed. All N-linked oligosaccharides have a common core pentasaccharide with the following structure: 39 In some glycoproteins, processing is limited, leaving “high-mannose” oligosaccharides; in other glycoproteins, extensive processing generates large oligosaccharides containing several kinds of sugar residues. There is enormous diversity among the oligosaccharides of N-linked glycoproteins. 40 Figure 16: Some examples of N-linked oligosaccharides. 41 The most common O-glycosidic (O-linked) attachment involves the disaccharide core β -galactosyl-(1→3)-α-N-acetylgalactosamine α-linked to the OH group of either Ser or Thr. Less commonly, glucose, galactose, mannose, and xylose form α-O-glycosides with Ser or Thr. Figure 17: Some common O-glycosidic attachments of oligosaccharides to glycoproteins (red). 42 A single protein may contain several N- and O -linked oligosaccharide chains, although different molecules of the same glycoprotein may differ in the sequences, locations, and numbers of covalently attached carbohydrates (the variant species of a glycoprotein are known as its glycoforms). This heterogeneity makes it difficult to assign discrete biological functions to oligosaccharide chains. In fact, certain glycoproteins synthesized by cells that lack particular oligosaccharide-processing enzymes appear to function normally despite abnormal or absent glycosylation. In other cases, however, glycosylation may affect a protein’s structure, stability, or activity. 43 • Oligosaccharides Help Define Protein Structure. Oligosaccharides are usually attached to proteins at sequences that form surface loops or turns. Since sugars are hydrophilic, the oligosaccharides tend to project away from the protein surface. Because carbohydrate chains are often conformationally mobile, oligosaccharides attached to proteins can occupy time-averaged volumes of considerable size. In this way, an oligosaccharide can shield a protein’s surface, possibly modifying its activity or protecting it from proteolysis. • Oligosaccharides Are Antigenic Determinants. The carbohydrates on cell surfaces are some of the best known immunochemical markers. For example, the ABO blood group antigens are oligosaccharide components of glycoproteins and glycolipids on the surfaces of an individual’s cells (not just red blood cells). 44