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Course MB151P100E THE CELL NUCLEUS –EXAMINATION 2016 Christian Lanctôt, Ph.D. Department of Cell Biology, Faculty of Science, Charles University in Prague [email protected], www.lanctotlab.org, Tel. 325 873 905 Time and place The exams will take place on: Monday, May 9th Monday, May 16th Tuesday, May 17th BIOCEV in the morning, Viničná 7 in the afternoon BIOCEV in the morning, Viničná 7 in the afternoon BIOCEV in the morning, Viničná 7 in the afternoon Format Oral examination. Duration: no more than 25-30 minutes. The candidate is asked 2 questions on topics that were covered during the lectures. These topics are summarized at the end of each lecture. The candidate provides a detailed answer (approx. 10-15 minutes per answer). Although students are encouraged to answer in English, the candidate can choose to answer in Czech. Grades What is tested is the student’s ability not only to answer a given question, but also to explain how the results that he/she describes were generated (choice of techniques, experimental design, interpretation of results, etc.). The evaluation takes into account answers to both questions. 1 – Excellent 2 – Good 3 – Average 4 - Fail Examples of questions A - Discuss the observation that eukaryotes have a chimeric genome. Fail 3 2 1 Unable to answer. Wrong answer. Consists of bacterial and archeal homologs. Consists of bacterial and archeal homologs. Archae-derived genes are involved in information processing. Bacterial-derived ones are involved in metabolism. At least one example provided for each. Consists of bacterial and archeal homologs. Archae-derived genes are involved in information processing. Bacterial-derived ones are involved in metabolism. At least one example provided for each. The mechanism of lateral gene transfer is discussed. B – List and discuss three possible functions of nuclear bodies. Fail 3 2 1 Unable to answer. Wrong answer. Reaction sites. Regulation. Storage. Reaction sites. Regulation. Storage. Examples for each: nucleolus (rRNA synthesis), PML (viral metabolism), speckles (splicing factors). Reaction sites. Regulation. Storage. Examples for each: nucleolus (rRNA synthesis), PML (viral metabolism), speckles (splicing factors). Describes an experimental result pointing to the function of the nuclear body, e.g. enlargement of speckles after inhibition of transcription. Course on The Cell Nucleus – Examination - Summer Semester 2016 1