* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Genetic Testing - Why, When and Whom
Genetic code wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Quantitative trait locus wikipedia , lookup
Gene therapy wikipedia , lookup
Tay–Sachs disease wikipedia , lookup
Neuronal ceroid lipofuscinosis wikipedia , lookup
Genetic drift wikipedia , lookup
Epigenetics of neurodegenerative diseases wikipedia , lookup
Fetal origins hypothesis wikipedia , lookup
History of genetic engineering wikipedia , lookup
Behavioural genetics wikipedia , lookup
Pharmacogenomics wikipedia , lookup
Heritability of IQ wikipedia , lookup
Genealogical DNA test wikipedia , lookup
Population genetics wikipedia , lookup
Human genetic variation wikipedia , lookup
Genetic engineering wikipedia , lookup
Designer baby wikipedia , lookup
DNA paternity testing wikipedia , lookup
Microevolution wikipedia , lookup
Medical genetics wikipedia , lookup
Genome (book) wikipedia , lookup
GENETIC TESTING – WHY, WHEN AND WHOM Denise Goh Li Meng Genetic Testing has become part of clinical medicine and its role will continue to grow. An awareness of the availability, advantages, limitations and interpretation of genetic testing is important to ensure its correct use and benefits to the patient. Contents • • • • • • • • • Definition Why When Whom How to achieve informed consent? Accuracy of genetic tests Interpretation of results Follow up of results of genetic tests (Post- testing genetic counseling) Special issues to pay attention to Bulletin 33; November 2003 MITA (P) No: 275/05/2003 1 Definition Genetic tests are laboratory techniques used to determine if a person has a genetic disorder or is likely to get the disorder. In Singapore, there are many genetic tests available on a clinical basis. Clinical genetic testing is mainly physician initiated viz. a patient presents with a complaint that the physician recognizes as a possibly genetic in origin, and orders or refers the patient for genetic testing. A small proportion is patient driven and this tends to be in the area of carrier, pre-natal and pre-symptomatic diagnosis. Why? The main reasons for genetic testing are 1. to confirm a specific diagnosis in a symptomatic individual (diagnostic testing) 2. to ascertain the risk of having a particular condition in an individual who is asymptomatic at the time of the testing (predictive testing) 3. to ascertain the risk of transmitting a condition (carrier testing) 4. to ascertain the risk of a fetus having a clinically significant genetic disorder (prenatal diagnosis) When? Genetic testing is offered when (1) there is suspicion that a gene contributes to the pathogenesis of the disease, and (2) when such testing is available. This is dependent on the type of genetic disorder involved. There are three main groups of genetic disorders: single gene disorders, chromosomal disorders and multifactorial disorders. 1. Single Gene Disorders A single gene disorder is caused by a change in a single gene. There are many different types of single gene disorders. Individually, they are usually rare but overall they affect ~2 percent of the population over a lifetime. If the gene for the disease has been identified, it is likely that genetic testing is available or will be available soon for clinical use. 2. Chromosomal Disorders Individuals with chromosomal disorders have either a deficiency or excess of a part or whole chromosome. As chromosomes contain genes, a chromosomal disorder causes problems because many genes are either deficient or in excess. Chromosomal disorders are relatively common. About half of the miscarriages that occur in the first trimester are due to chromosomal defects, and about 0.7 per cent of babies are born with a chromosomal defect. An example is Down’s syndrome. Common tests done to look for chromosomal disorders include a karyotype and fluorescent in-situ hybridization (FISH). Bulletin 33; November 2003 MITA (P) No: 275/05/2003 2 3. Multifactorial Disorders These are a group of diseases that arise due to multiple factors that include both genetic and environmental factors. They can cause many types of diseases ranging from birth defects in babies to common disorders in adults e.g. heart disease, diabetes mellitus. It is estimated that this group of disorder affects more than 60 percent of the population. Genetic testing is not usually available for this group of diseases as the effect of genetic variation on disease is not yet fully understood. Whom? Anyone who is an adult capable of making an informed consent can undergo genetic testing. There are, however, certain vulnerable groups that may need to be protected. These include 1. Minors (children) 2. Mentally incompetent 3. Individuals with language difficulties 4. Prisoners and students Minors with genetic diseases tend to fall into one of these groups 1. Symptomatic at diagnosis 2. Asymptomatic at evaluation, at risk of developing disease childhood/adulthood, availability of intervention or treatment during childhood 3. Asymptomatic at evaluation, at risk of developing disease adulthood, availability of intervention or treatment only during adulthood 4. Asymptomatic at evaluation, at risk of developing disease, no intervention or treatment available Minors in groups (1) and (2) can be tested as the results of the tests are likely to be directly benefit them. Minors in groups (3) and (4) should not be tested during childhood as prior knowledge usually has no direct benefit to the child, whereas the person giving consent may have a vested interest in the outcome of the genetic tests. The counter argument is that science is rapidly advancing and intervention or treatment in childhood may become available. To maximally benefit from such advancement, there is a need to know that one has the disease. In balance, effort must be made to protect the privacy of the child and his right not to know his genetic risk. Genetic testing of mentally incompetent individuals raise similar issues as the legal guardian who provides consent may benefit more from the results than the affected themselves. Non-educated persons and individuals with language issues e.g. deaf, foreigners are another vulnerable group as they may not be able to comprehend and hence not capable of truly informed consent. Prisoners and students are vulnerable as they may feel coerced to consenting to participating in genetic tests. Bulletin 33; November 2003 MITA (P) No: 275/05/2003 3 How to achieve informed consent Informed consent is the process by which a person is made fully aware of their options and participate in his/her choices about health care. To achieve informed consent in genetic testing is challenging because it involves many complex issues that may not be intuitive to the physician or patient. Hence, pre-testing genetic counseling given by an appropriately trained person is both prudent and essential. The aims of pre-testing genetic counseling are to explain the following to the patient/guardian 1. Genetic testing is voluntary and consent is required to proceed. 2. Time should be taken to ask all questions needed to make an independent personal decision. After consent is given, withdrawal of consent can be done at any time or the disclosure of the results postponed 3. The major medical facts of the disorder (diagnosis, prognosis, treatments available, inheritance pattern, and risks of recurrence in the family) 4. The implications of genetic testing (implications to other family members, detection of non-paternity, and possibility of psychological stress) 5. Sample required and possible side effects of the sample taking procedure. 6. Test procedure and expected turn around time 7. Accuracy of test results 8. Confidentiality of results 9. Alternative to gene testing Only after informed consent, is the sample is taken and sent for testing. The turn around time varies from days to weeks depending on the test and laboratory involved. Accuracy of genetic tests Accuracy in genetic testing is dependent on several factors 1. Integrity of the diagnostic chain i.e. ensuring no sample switch, contamination etc. 2. The test methodology. Test methodologies are broadly grouped into those based on DNA, RNA, chromosomes and/or proteins. Each group has its advantages and limitations, and these have implications on the interpretation of the results. Interpretation of results One of the biggest challenges in genetic testing is accurate interpretation of the results. This is greatly dependent upon experience and having up to date knowledge. In the context of diagnostic testing (i.e. a test used to identify or confirm the diagnosis of a disease in an affected individual), a positive test result is relatively straightforward to interpret. It confirms the clinical diagnosis, may give a prediction of the course of illness, can lead to a better choice in treatment and can be used to identify at-risk Bulletin 33; November 2003 MITA (P) No: 275/05/2003 4 family members. If an affected person tests negative, this does not imply that the clinical diagnosis is wrong. This negative test result may have arisen because (1) a mutation is present but test could not find it or (2) another gene is causing the disease. What it does mean is that outlook and treatment is not tailored and at risk family members are not likely to benefit from predictive testing. In the context of predictive testing (i.e. a test used to determine if an asymptomatic person is at risk of developing a genetic disorder), its utility hinges on whether we know the mutation in the family. If we know the mutation in the family, the genetic tests serves to answer the question “Does this individual have the family mutation?”. If this person tests negative, then he/she is very unlikely to develop that disease. If this person tests positive, then he/she has a risk of developing that disease. However, this risk may be complicated to quantify because (1) the certainty of having disease may not be 100% (nonpenetrance), (2) lack of genotype-phenotype correlation, (3) modification by preventive measures. If we don’t know the mutation in the family, the genetic tests serves to answer the question “Is there a significant mutation present in this gene?”. If this person tests positive, then he/she has a risk of developing that disease. Similar to the above situation, this risk may be complicated to quantify because (1) the certainty of having disease may not be 100% (non-penetrance), (2) lack of genotype-phenotype correlation, (3) modification by preventive measures. If the person tests negative, this does not exclude the possibility of still being at because (1) a mutation present but test could not find it or (2) another gene is causing the disease. Follow up of results of genetic tests (Post- testing genetic counseling) As illustrated above, interpretation of genetic tests are complicated and hence a follow up visit is recommended so that a qualified person can explain the results and implications of the test in simple layman language. The patient’s reaction, expectations and questions will have to be addressed and several visits may be necessary. Special issues to pay attention to Genetic testing not only has medical implications, it also has many ethical, social and legal implications. With the evolution and advancement of genetic testing technology, issues raised include • Confidentiality • Equitable accessibility to testing • Revelation of non paternity and undisclosed adoption • Risks for discrimination • Risks for psychological stress e.g. guilt, anxiety, self doubt, ostracized, despair because no treatment is available etc. Bulletin 33; November 2003 MITA (P) No: 275/05/2003 5 There are no easy answers to these solutions. Several developed countries have come up with their own set of recommendations and Singapore is currently also developing her own especially since the governance and management of bioethical issues is critical to her development in the biomedical sciences. The Bioethics Advisory Committee, appointed by the Cabinet in December 2000, is tasked to address the potential ethical, legal and social issues arising from biomedical sciences research in Singapore. This includes the issue of genetic testing and its recommendations on this matter are expected with the next year. Diagnostic testing Test positive Test negative • Confirm the diagnosis • May give a prediction of the course of illness • Can help choose the better treatment • Can Identify at-risk family members • Does not exclude the possibility of having the disease. – Mutation present but test could not find it – Another gene is causing the disease • Outlook and treatment not tailored • At risk family members not likely to benefit from predictive testing Predictive Genetic Testing Family mutation known • Tests positive: has a risk of developing that disease – What is the certainty of disease? (Penetrance) – How severe? (Genotypephenotype correlation) – Do preventive measures work? – Is there early treatment? • Tests negative: unlikely to develop that disease Bulletin 33; November 2003 MITA (P) No: 275/05/2003 Family mutation not known • Tests positive: has a risk of developing that disease – What is the certainty of disease? (Penetrance) – How severe? (Genotype-phenotype correlation) – Do preventive measures work? – Is there early treatment? • Tests negative: Does not exclude the possibility of having the disease. – Mutation present but test could not find it – Another gene is causing the disease 6