Download MagNA Lyser Instrument

Ordering Information
Cat. No.
Product
03 358 976 001
MagNA Lyser Instrument (230 Volt)
03 358 968 001 MagNA Lyser Instrument (110 Volt)
(Instruments supplied with rotor and rotor cooling block)
03 358 941 001 MagNA Lyser Green Beads (100 tubes)
System Description
Automate with an easy-to-use instrument
Fully automated sample preparation
on the PCR Workflow System
Homogenize up to 16 samples in just a few seconds.
Save valuable lab space with a small benchtop instrument.
Related Products
Cat. No.
Product
03 186 229 001 MagNA Pure LC DNA Isolation Kit II (Tissue)
03 172 627 001
MagNA Pure LC mRNA Isolation Kit II (Tissue)
03 330 591 001 MagNA Pure LC RNA Isolation Kit III (Tissue)
03 264 785 001
MagNA Pure LC DNA Isolation Kit III (Bacteria, Fungi)
03 604 721 001 MagNA Pure LC RNA Isolation Tissue Lysis Buffer – Refill (70 ml)
Roche Applied Science
Part of Roche Diagnostics
MagNA Lyser Instrument
Reduce hands-on time by replacing the mortar and pestle
and other manual methods.
Integrate your workflow with the automated nucleic acid
isolation of the MagNA Pure LC Instrument.
Versatile, efficient, and rapid pre-preparation
Figure 6
Components of the
system.
Perform consistent and reproducible sample disruption.
Process many different sample types.
Prevent nucleic acid degradation with the benchtop
cooling unit.
Ease your setup with a removable rotor and prefilled
disposable vials.
MagNA Lyser Workflow
For detailed information, visit
www.pcr-workflowsystem.com
www.magnapure.com
Figure 7
1. Add your sample
and lysis buffer to
the MagNA Lyser
Green Beads.
2. Homogenize with
the MagNA Lyser
Instrument.
3. Centrifuge to
pellet the debris.
4. Proceeed with the
supernatant to
prepare nucleic
acids or proteins.
0903-03604411
or contact your local representative.
Trademarks:
MagNA Pure, MagNA Lyser, LightCycler, and
the MagNA Pure Logo are trademarks of a
member of the Roche Group.
The technology used for the LightCycler System
is licensed from Idaho Technology Inc.,
Salt Lake City, UT, USA.
Roche Diagnostics GmbH
Roche Applied Science
Nonnenwald 2
82372 Penzberg
Germany
Start the Ball Rolling
with Automated Tissue Homogenization
The MagNA Lyser Instrument
Automated tissue homogenization
1 x 106
Processing conditions
5 x 106
1 x 107- 5 x 107
Sample material
Time settings
Cooling
(10 mg)*
(seconds)
(between
the runs)
Spleen
2 x 25
Speed
90
Average yield
Average purity
(µg)***
(OD 280/260 nm)***
6,000
30–40
Liver
25
-
6,000
16–18
1.8
Lung
2 x 25
90
6,000
25
1.8
Kidney
25
-
6,000
20
1.8
Rarely expressed targets in small numbers of target
cells, as seen in experiments about minimal
residual diseases, are difficult to detect. Increasing
the cell number can improve sensitivity and lead
to accurate results. Without the MagNA Lyser preprocessing, the MagNA Pure mRNA HS Kit can
efficiently obtain mRNA from a maximum of 1 x 107
white blood cells (WBCs), as shown in research
studies with human samples. However, using
greater cell numbers results in a saturation effect
with quantitative assays (Figure 3).
Homogenization of the lysate with the MagNA Lyser
Instrument prior to the purification eliminates
the amplification saturation at 1 x 107 cells and allows
the use of up to 2.5 x 107 WBCs (Figure 4 and 5),
enhancing the analytical sensitivity of the assay.
Figure 2
Gel electrophoresis from total RNA isolated
from tissue homogenized with the MagNA
Lyser Instrument, using the MagNA Pure LC
RNA Kit III (Tissue).
1.9
28 S rRNA
18 S rRNA
Maize leaves **
20
-
5,000
10
n.d.
Maize polenta **
20
-
5,000
8
n.d.
Tortilla chips **
20
-
5,000
1
n.d.
11 kb
5 kb
5 kb
le
av
es
M
ai
ze
M
ai
ze
Lu
ng
Li
ve
r
Sp
le
en
DNA
M
ar
ke
r
n.d.
le
av
es
***
M
ar
ke
r
**
RNA/mRNA
Aliqout containing 10 mg sample material (here mouse and
food samples) was taken for the DNA purification
using the MagNA Pure LC DNA Isolation Kit II (Tissue),
(see pack insert)
Centrifugation after the homogenization for 5 minutes at
2,200 x g
Yield and purity strongly depend on the condition of the
sample material
not determined
Data kindly provided by Dr. Peterhänsel, RWTH Aachen,
Germany
Ki
dn
ey
*
Figure 1
Gel electrophoresis
from genomic DNA
isolated from tissue
homogenized with the
MagNA Lyser
Instrument, using the
MagNA Pure LC DNA
Kit II (Tissue).
Marker: DNA Marker III
Figure 3
mRNA was purified from different amounts of human
white blood cells with the MagNA Pure mRNA HS Kit.
G6PDH was amplified using the LightCycler t(9;22)
Quantification Kit (see text beside).
Eliminate sensitivity barriers with
increased sample input
Li
ve
r
DNA
Sp
le
en
Refer to the following tables for guidelines on setting up
your homogenization
Sample
material
Time
settings
Cooling
(10 mg)*
(cycles/seconds)
(between/after
the runs in seconds)
Speed
Average
yield (mg)
Average purity
Spleen
2 x 50
90
6,500–7,000
30–40
1.9
Liver
50
-
6,500–7,000
13–17
2.0
1 x 106
5 x 106
5 x 106- 5 x 106
2.5 x 106, 5 x 107
with the MagNA Lyser
Instrument
Figure 4
mRNA was purified from different amounts of human
white blood cells with the MagNA Pure mRNA HS Kit.
The lysates from 2.5 x 107 cells and 5 x 107 cells were
homogenized with the MagNA Lyser Instrument (2x50
seconds with 90 seconds cooling in between) prior to
the mRNA purification. G6PDH was amplified using the
LightCycler t(9;22) Quantification Kit (see text beside).
(OD 280/260 nm)**
(total RNA)**
Thymoid tissue
60
90
6,500
n.d.
n.d.
Heart
60
90
6,500
n.d.
n.d.
Abdominal fat
60
90
6,500
n.d.
n.d.
Aorta
60
90
6,500
n.d.
n.d.
Other samples
1+n x 50
90
6,500–7,000
-
-
Figure 5
Scalability from 1 x 106 cells to 2.5 x 107 cells is
represented in the graph and the table of the
relationship between crossing points and cell
numbers. The limitation of cell input is indicated
by no change in crossing point with increased
cell number (see text beside).
crossing
point
25
Cell number
Log (cell number)
Crossing point
5 x 107
7.7
20.3
23
2.5 x 107
7.4
20.3
22
1 x 107
7.0
21.8
5 x 106
6.7
22.4
1 x 106
6.0
24.4
24
*
Aliquot containing 10 mg sample material (here mouse
and human research samples) was taken to purify RNA
either with the MagNA Pure LC RNA Isolation Kit III (Tissue)
or the MagNA Pure LC mRNA Isolation Kit II (Tissue)
homogenized with the MagNA Lyser Instrument.
** Yield and purity strongly depend on the condition of the
sample material. The yield for mRNA was not determined.
21
20
19
5.8
6.3
6.8
7.3
7.8
Log
(cell number)