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CLASSICAL CONDITIONING Classically Conditioning a Salivary Response in Dogs by Sean M. McEvoy Student ID#201006224 A laboratory report presented to J. Wright in Psychology 210 Learning Department of Psychology St. Francis Xavier University January 24, 2014 CLASSICAL CONDITIONING 2 Classically Conditioning a Salivary Response in Dogs Classical conditioning involves the presentation of a conditioned stimulus (CS), which was initially a neutral event such as a light or a tone and does not create a response in any manner from the individual or individuals which are being tested. In these tests or instances the CR is paired with an unconditioned stimulus (US) which does elicit some sort of natural response from the participants which is called the unconditioned response (UR). To eventually create and develop a CR in Classical conditioning, the US and CS should be paired multiple times consecutively according to the research of the famed Ivan Petrovich Pavlov After multiple pairings of the CS first, followed by the US with the creation of a given UR, the US can be taken away with the CS being presented on its own in a solo manner. The CS will then produce responses which are very similar to the UR, which can be labeled as a conditioned response (CR). When this series of events is demonstrated, it can be stated the Classical conditioning or Pavlovian conditioning has taken place. This theory and series of steps would outline the process of acquisition which is aimed to be demonstrated in numerous experiments which have been conducted for the purpose of this research report, in which a certain CR is acquired by test individuals (in our case virtual dogs). The other condition that we are testing to observe in these trials is that of Experimental Extinction, or simply extinction. The process of extinction is when the CR gradually or rapidly disappears from the test subjects after the CS is not presented with any form of positive or negative reinforcement to encourage a continuation of the specific behavior. Numerous experiments were conducted in the past by Pavlov, such as one that added a solution of acid to a serious of dogs mouth to elicit a predicted defensive reaction from the animals. In our specific experiment, dealing entirely with virtual test subjects run through a simulator program, yet the process and theory behind the experiments, and thus the expected CLASSICAL CONDITIONING 3 hypothesized results for the testing remains the same. Through various experiments, we are testing for the acquisition and extinction of a conditioned response in our virtual dogs following being tested in various conditions and various different methods. It is hypothesized that in experiments where the CS is presented at an appropriate rate and timing along with other factors, the acquisition rate will increase and thus so will the mean drops of saliva from the dog. However it is also predicted that in extinction experiments where the CS is not presented on a regular basis or presented with proper reinforcement, we will witness gradual to rapid extinction of the response (drops of saliva) from the dog. These hypotheses are made based on the many works and experiments of Pavlov. After running these multiple simulated tests and experiments, as well as plotting acquisition and extinction graphs, we should be able to form a better picture of the impacts of Classical Conditioning. Method Subjects Subjects were 56 virtual dogs………………………………………………… ……………….. ………………. ……………………. …………………………. ……………. ……… Apparatus A program called Classical Conditioning was used……………………………… ……………….. ………………. ……………………. …………………………. ……………. …………… … ………………………………………… ……………….. ………………. ……………………. …………………………. ……………. ……… Procedure CLASSICAL CONDITIONING 4 Experiment 1 used……… ………………… ………………… …………… ….. ………………. ……………………. …………………………. ……………. …………… … ………………………………………… ……………….. ………………. ……………………. …………………………. ……………. ……… Results The first experiment set out to see how the number of acquisition tests performed on our individual test subject virtual dogs, would have an impact on the number of saliva drops that the dog would produce. For the first four trials (10, 20, 30, and 40 tests) we see a steady plotted increase in the mean amount of saliva drops produced by the dogs. However in the fifth trials ( 5 50 acquisition tests), we see that a point of saturation has appeared to have been reached. In the fifth trial we see a less sleep slope then the other four which breaks the trend, suggesting that the peak number of trials for maximum acquisition of a CR is between 40 and 50 trials. (see Figure 1). Alternatively, the second part of experiment one focused on the experimental extinction data. In streams for the 10, 20, 30, and 50 trials, we see a steady steep rapid decline in mean drops of saliva produced by the dog test subjects, eventually reaching at or near zero. The exception to this was the forty stream, which had a much less steep slope and remained well above zero (See Figure 2). Explanations for these results may include some aversion the dogs have to these number of trials, a certain resistance which allows them to maintain the acquired response of saliva drips. In experiment two, the strength of the CS was varied, in this case the strength of the decibels which was exposed to the dog test subjects to see the effect on acquisition of saliva drops. At a CS decibel level of 50, we saw a low but steady incline in the production of saliva. At CS decibel level 60 we again saw a steady level of incline but this time at a higher quantity. CLASSICAL CONDITIONING 5 Finally at CS level 80, we see an immediate increase in saliva levels almost instantly during the first few trials. The spike continues before leveling off around the 25 trial mark (See Figure 3). This experiment clearly shows a direct correlation between increased CS decibel levels, and increased CR in saliva quantity. In experiment three we tested Discussion The results of the current study support the hypotheses. It was hypothesized that….. … ……… ……….. ………………. ……………………. …………………………. ……………. ………… … … ………………………………………… ……………….. ………………. ……… CLASSICAL CONDITIONING 6 References American Psychological Association. (2009). Publication manual of the American Psychological Association (6th ed.). Washington, DC: American Psychological Association. Carlson, N. R., & Buskist, W. (1997). Psychology: The science of behavior (5th ed.). Boston: Allyn and Bacon. Gazzaniga, M. S., & Heatherton, T. F. (2003). Psychological science. New York: W. W. Norton and Company. CLASSICAL CONDITIONING Figure 1. Drops of saliva during acquisition as a function of number of acquisition trials. 7