Download The Role of Laboratory Instructors

LABORATORY
2
Introduction to Microscopic Anatomy
Post -lab answer Key
1. Complete, or respond to, the following questions:
a. The _____ is a lens under the stage that concentrates the light:
condensor
b. The area of a slide seen when looking through a microscope is the:
field of view
c. If the stage has a device that moves the slide from side to side
and up or down, the stage is called a(n) _______________
stage.
mechanical stage
d. The ability to distinguish between closely positioned objects is
called:
resolution
e. If after focusing using a low power objective lens only the fine adjustment
needs to be used to focus under a higher power, the microscope is said to be:
parfocal
f. If a microscope has a 10X ocular lens and the objective lens in
use is the 100X, what is the total magnification achieved?
1000X
g. The distance from the bottom of the objective lens to the specimen is
called the:
working distance
h. The set of lenses closest to the viewer’s eyes are:
ocular lenses
i.
What device allows the user to alter the amount of light that passes
through a specimen from a light source?
lens diaphragm
j.
You are looking into the microscope and are surprised to see
that only half of the field is illuminated. What is the probable cause?
Objective lens not in place
1
k. You are viewing a specimen using a microscope and you attempt to
change the position of the specimen in the field using the mechanical
stage. You are surprised when the slide does not move. What is the
probable cause?
Slide not in holder properly
l.
The letter “J” appears in this orientation under the cover slip of a
microscope slide. How will this letter appear when viewed using a
microscope? (Draw it in the space):
Backwards and upside down J
2. Indicate which type of preparation (whole mount, smear, longitudinal section,
cross section or squash) you would use in the following situations:
To view the lumen (opening) in the center
of a blood vessel
cross section
To count sperm cells present in a sample
of seminal fluid
smear
To view an entire, intact human embryo
whole mount
To view the internal tissues of a tooth
from the superior portion to the inferior
portion
longitudinal section
To release pathogens from infected
cells in preparation for microscopy
squash
3. Using your textbook, determine the function(s) of the following:
Plasma Membrane:
Separates extracellular compartment from
intracellular compartment; transport of materials
Cytoplasm:
material surrounding mucleus and within plasma
membrane; area where most cell activities take place
Nucleus:
the control center of the cell
2
4. Indicate whether the tissue/cell below is derived from ectoderm (EC),
mesoderm (ME), or endoderm (EN):
ME
blood
ME
lymphatic tissue
EC
skin (epidermis)
ME
bones
EC
brain and spinal cord
EC
adrenal medulla
ME
heart
EC
melanocytes
ED
lining of the respiratory tract
ED
thyroid gland
3