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Post lab worksheet lab 2 1. Complete, or respond to, the following questions: a. The _____ is a lens under the stage that concentrates the light.: condensor b. The area of a slide seen when looking through field of view a microscope is the: c. If the stage has a device that moves the slide from side to side and up or down, the stage is called a(n) _______________ stage. d. The ability to distinguish between closely positioned objects is called: mechanical resolution e. If, after focusing using a low power objective parfocal lens only the fine adjustment needs to be used to focus under a higher power, the microscope is said to be: f. If a microscope has a 10X ocular lens and the objective lens in use is the 100X, what is the total magnification achieved? 1000X g. The distance from the bottom of the objective Lens to the specimen is called the: working distance h. The set of lenses closest to the viewer’s eyes ocular lens are: i. What device allows the user to alter the amount of light that passes through a specimen from a light source? j. You are looking into the microscope and are surprised to see that only half of the field is illuminated. What is the probable cause? Objective lens not fully in place You are viewing a specimen using a microscope and you attempt to change the position of the specimen in the field using the mechanical stage. You are surprised when the slide does not move. What is the probable cause? slide not properly placed in the slide holder k. iris diaphragm l. The letter “J” appears in this orientation under the cover slip of a microscope slide. How will this letter appear when viewed using a microscope? (Draw it in the space): __________________________ 2. Indicate which type of preparation (whole mount, smear, longitudinal section, cross section or squash) you would use in the following situations: To do a blood cell count smear To view the lumen (opening) in the trachea cross section To view an entire, intact human embryo whole mount To view the internal tissues of a tooth longitudinal section from the superior portion to the inferior portion To release pathogens from infected cells in preparation for microscopy squash 3. Using your textbook, determine the function(s) of the following: Plasma Membrane:____________________________________________________ ______________________________________________________________ Cytoplasm:_____________________________________________________ Nucleus: _____________________________________________________ ______________________________________________________________ 4. Indicate whether the tissue/cell below is derived from ectoderm (EC), mesoderm (ME), or endoderm (EN): EN liver EC skin (epidermis) ME lymphatic tissue ME heart EC brain and spinal cord EC adrenal medulla ME bones ME blood ED lining of the respiratory tract ED thyroid gland