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HAPPY TUESDAY
Bellwork:
1. Collect Lab Materials (Goggles and Aprons)
2. Turn in HW - Page 43 (Franklin, Watson, & Crick)
3. If you were absent Friday, please turn in “Make-Up Work”.
4. QUICKWRITE – 50 word quickwrite explaining what Watson,
Crick, and Franklin discovered. Also, describe the following
picture and its relevance.
Unit 3 – DNA & Replication
All Parts Due TOMORROW (10/21/15)
1. DNA
2. Nucleotide
3. Nucleic Acid
4. Deoxyribose
5. Nitrogenous Base
6. Hydrogen Bond
7. Adenine
8. Cytosine
9. Guanine
10.Thymine
11.Purine
12.Pyrimidine
13.Double Helix
14.Antiparallel
15.Semi-Conservative
16.DNA Polymerase
17.Helicase
18.DNA Replication
19.Complementary Strand
Pg 39
Essential Question
PG 44
Where can we find
DNA?
Standard
B.6A – Identify components of DNA, and describe how
information for specifying the traits of an organism is carried in
the DNA
Procedure
1.
Obtain a small cup of Salt Water. Swish the Salt Water around in your mouth
for 1 to 2 full minutes. As you swish, gently and continuously scrape the sides
of your cheeks with your teeth to help release your cheek cells.
2.
Spit the Salt Water (with your cheek cells) into a test tube. Dispose of the
cup.
3.
Hold the test tube at an angle and add one pipette full of cell lysis solution to
your cheek cell solution.
4.
Cap your test tube with your thumb and invert it 5 times to mix the two
solutions.
5.
Allow the mixture to sit for 15 minutes.
6.
Work on the Page 44 while you wait.
Page 44: DNA Extraction Lab Questions
*Write the question and answer in complete sentences*
1. What is the purpose of DNA?
2. What did the DNA look like? Relate what you know about the chemical structure of DNA to what you
observed today.
3. A banana is composed of eukaryotic cells. Where is its DNA located?
4. Where is the DNA located in a prokaryotic cell? Why?
5. What is the monomer of DNA?
6. What is this monomer composed of?
7. How does the genetic code (DNA) differ from organism to organism?
8. Why is it important for scientists to be able to remove DNA from an organism? List two reasons.
Procedure cont’d
7.
After 10 minutes, hold your test tube at an angle, add ethyl alcohol to
your test tube (Add one pipette full of alcohol to the test tube) by
letting it run gently down the side of the test tube. You should have
two distinct layers. Do not mix the cheek cell solution with the
alcohol!!!
8. Watch as cobweb-like strands of DNA begin to clump together where
the alcohol layer meets the cheek cell solution.
9. Use a plastic pipette (the same one used for the ethyl alcohol) to
transfer your DNA into a centrifuge tube. To do so, place the pipet
near the DNA and draw the DNA and some alcohol into the pipet.
10. Continue working on Page 44 If you do not finish in class, it is
homework. It will be due TOMORROW when you get to class.
Page 44: DNA Extraction Lab Questions
*Write the question and answer in complete sentences*
1. What is the purpose of DNA?
2. What did the DNA look like? Relate what you know about the chemical structure of DNA to what you
observed today.
3. A banana is composed of eukaryotic cells. Where is its DNA located?
4. Where is the DNA located in a prokaryotic cell? Why?
5. What is the monomer of DNA?
6. What is this monomer composed of?
7. How does the genetic code (DNA) differ from organism to organism?
8. Why is it important for scientists to be able to remove DNA from an organism? List two reasons.