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Transcript
Name: _________________________________________________________________________
Period: ________
DNA Replication, Transcription and Translation
DIRECTIONS: Follow the steps and answer the questions in order. YOU NEED PAGE 7 OF YOUR NOTES!!!
PROCEDURE:
1) STEP ONE: The letters below represent a piece of DNA fill in the complimentary bases to make an mRNA strand. Then
construct the amino acid chain from the mRNA.
a)
b)
c)
d)
e)
f)
g)
h)
i)
j)
k)
l)
m)
n)
o)
T - ______
A - ______
C - ______
A - ______
G - ______
C - ______
T - ______
G - ______
C - ______
C - ______
T - ______
C - ______
A - ______
T - ______
T - ______
 amino acid = __________________________________
 amino acid = __________________________________
 amino acid = __________________________________
 amino acid = __________________________________
 amino acid = __________________________________
p) What makes up the backbone of the DNA molecule? ____________________________________
q) What is the monomer of DNA? ____________________________________
2) STEP TWO:
a)
What is the enzyme that “unzips” the DNA ____________________________________
b) What is DNA? Why is it important?
c)
Define DNA replication
3) STEP THREE:
a)
What enzyme adds the nucleotides to the new growing chain of DNA in the cell? _____________________________
4) STEP FOUR:
a)
Define transcription –
b) What is mRNA? What does it do?
c)
Fill out the following chart, checking the box if the info pertains to the DNA and/or RNA
DNA
Ribose Sugar Present
Deoxyribose Sugar Present
Phosphate Group Present
Adenine Present
Guanine Present
Thymine Present
Cytosine Present
Uracil Present
Double Stranded
STEP FIVE:
d) Define Translation –
e)
Define Codon –
f)
Define Anticodon –
g) What does tRNA do? –
GO TO THE NEXT PAGE!!!
RNA
STEP SIX:
The letters below represent a piece of DNA that is ready to be transcribed.
Part 1: Fill in the complimentary bases to make an mRNA strand.
T - ______
C - ______
T - ______
A - ______
C - ______
A - ______
G - ______
C - ______
T - ______
G - ______
C - ______
C - ______
T - ______
C - ______
A - ______
T - ______
T - ______
Part 2: After completing your mRNA strand, translate the mRNA into an amino acid sequence below. Circle
the start codon (above). Begin translating at the start codon – look for it!
Amino acid sequence = ______________________________________________________________________
Part 3: If when transcription occurred you got the following mRNA instead:
AGAUGCGACGGAGUAA
What happened (how is it different)? _____________________________________________________