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Name: _________________________________________________________________________ Period: ________ DNA Replication, Transcription and Translation DIRECTIONS: Follow the steps and answer the questions in order. YOU NEED PAGE 7 OF YOUR NOTES!!! PROCEDURE: 1) STEP ONE: The letters below represent a piece of DNA fill in the complimentary bases to make an mRNA strand. Then construct the amino acid chain from the mRNA. a) b) c) d) e) f) g) h) i) j) k) l) m) n) o) T - ______ A - ______ C - ______ A - ______ G - ______ C - ______ T - ______ G - ______ C - ______ C - ______ T - ______ C - ______ A - ______ T - ______ T - ______ amino acid = __________________________________ amino acid = __________________________________ amino acid = __________________________________ amino acid = __________________________________ amino acid = __________________________________ p) What makes up the backbone of the DNA molecule? ____________________________________ q) What is the monomer of DNA? ____________________________________ 2) STEP TWO: a) What is the enzyme that “unzips” the DNA ____________________________________ b) What is DNA? Why is it important? c) Define DNA replication 3) STEP THREE: a) What enzyme adds the nucleotides to the new growing chain of DNA in the cell? _____________________________ 4) STEP FOUR: a) Define transcription – b) What is mRNA? What does it do? c) Fill out the following chart, checking the box if the info pertains to the DNA and/or RNA DNA Ribose Sugar Present Deoxyribose Sugar Present Phosphate Group Present Adenine Present Guanine Present Thymine Present Cytosine Present Uracil Present Double Stranded STEP FIVE: d) Define Translation – e) Define Codon – f) Define Anticodon – g) What does tRNA do? – GO TO THE NEXT PAGE!!! RNA STEP SIX: The letters below represent a piece of DNA that is ready to be transcribed. Part 1: Fill in the complimentary bases to make an mRNA strand. T - ______ C - ______ T - ______ A - ______ C - ______ A - ______ G - ______ C - ______ T - ______ G - ______ C - ______ C - ______ T - ______ C - ______ A - ______ T - ______ T - ______ Part 2: After completing your mRNA strand, translate the mRNA into an amino acid sequence below. Circle the start codon (above). Begin translating at the start codon – look for it! Amino acid sequence = ______________________________________________________________________ Part 3: If when transcription occurred you got the following mRNA instead: AGAUGCGACGGAGUAA What happened (how is it different)? _____________________________________________________