Supplementary Table 4. COMPARISON OF DYNAMIC AND ADAPTIVE RESPONSE OF S. cerevisiae WITH OTHER ORGANISMS Pathway/ Biological Process Reference behavior in S. cerevisiae Closer Organism Behavior of process in closer organism Either Glycosidase-I or Glycosidase-II is required for growth of S. cerevisiae cells. [1,2,3,4] Cell wall organization Meiosis Glycosidase-I does not play role in outer chain formation or mannosylation and elongation of oligosaccharide residues in cell wall organization. [4,5] S. cerevisiae has two silent mating cassettes (HML & HMR) and an active MAT locus. The morphogenesis is regulated by MAPK signaling pathway. [6,7,8,9] Candida glabrata Candida glabrata Candida glabrata C. glabrata has two silent mating cassettes and an active MAT locus and undergoes mating-type interconversions via a Ho-type endonuclease, regulated by a MAP kinase cascade. Ideal temperature for Pheromone stimulated growth is between 24º C and 37º C. [7,10] Only diploid cells of S. cerevisiae shows bipolar budding pattern. [11,12] More distant organism Candida albicans Candida albicans Kluyveromyces lactis Both haploid and diploid cells of K. lactis shows bipolar budding pattern. Candida albicans Behavior of process in more distant organism Glycosidase-I AND Glycosidase-II is required for growth of C. albicans cells. Glycosidase-I or Glycosidase-II play important role in mobility of β-Nacetylhexosaminidas e to initiate outer-Nchain elongation in cell wall organization. C. albicans has one MAT locus and a mating-type pleiotropic switching event is required for mating to occur. Pheromone causes smoothing, the initial step in the mating process, only in a/a cells expressing the opaque phenotype. Does not grow at 37º C. Axial budding pattern. Supplementary Table 4 continued. Switching from axial to bipolar Pseudohyphal mode is required growth for germ tube emission.  Thiamine metabolism Nonhomologous end-joining (NHEJ) recombination Thiamine biosynthetic pathway component plays dual role, cooperating with repair mechanisms in mitochondria. [13,14,15] Haploid strain performs NHEJ efficiently and diploid strain performs NHEJ inefficiently. [16,17] Illegitimate recombination (IR) by NHEJ pathway occurs at rate of 15 transformants/μg  IR mechanism is based on microhomology and the target site is near to the consensus sequence for TOP1 binding (Topoisomerase-1). [14,18,19] Candida albicans Arabidopsis thaliana Switching from axial to bipolar mode is required for germ tube emission because the mechanism requires a polar budding pattern. Thiamine biosynthetic pathway component probably plays dual role, cooperating with repair mechanisms. Candida glabrata Candida glabrata Candida glabrata IR mechanism is based on microhomology and the target site is near to the consensus sequence for TOP1 binding. Yarrowia lipolytica Pseudomonas fluorescens Switch from axial to bipolar mode is not necessary because Y. lipolytica shows budding pattern in haploid and diploid form. Thiamine biosynthetic pathway component does not play any role in repair mechanisms. Kluyveromyces lactis NHEJ transcription is not regulated by cell type (both haploid and diploid show same efficiency in repair). Kluyveromyces lactis Illegitimate recombination (IR) by NHEJ pathway occurs 1000 fold faster than in S. cerevisiae. Kluyveromyces lactis IR mechanism is not based on microhomology and the target site is not specific to the consensus sequence for TOP1 binding. [14, 59,70] Supplementary Table 4 continued. Mitotic and ORF IR Nonare equally homologous compromised by end-joining HR (homologous (NHEJ) recombination) and recombination NHEJ.  Isoleucine biosynthesis One carbon pool by folate 1-butanol production flux comes mostly from an intermediate of the isoleucine biosynthesis pathway produced through the use of threonine. [20,21,22,23] One-carbon pool in cytoplasm required for synthesis of purines, thymidylate and regeneration of methionine. [24,25,26] Candida glabrata Escherichia coli Rattus norvegicus Mitotic and ORF IR are equally compromised by HR and NHEJ. 1-butanol production flux comes mostly from an intermediate of the isoleucine biosynthesis pathway produced through the use of threonine. One-carbon pool in cytoplasm required for synthesis of purines, thymidylate and regeneration of methionine. Kluyveromyces lactis Laptospira interrogans, Methanococcus jannaschii, Geobacter sulfurreducens Arabidopsis thaliana Mitotic (cell cycle error) IR occurs 6 folds more frequently than in ORFs (transcription error). 1-butanol production is only observed when threoninemediated isoleucine biosynthesis is shut down and pyruvate mediated isoleucine biosynthesis is overexpressed. [5, 29, 56, 68] One-carbon pool in cytoplasm is only requiring for regeneration of methylation. It is not required for purine synthesis. Supplementary Table 4 continued. Kluyveromy ces lactis Production of different terpenoids from the same substrate has a flux and a time course similar to that in S. cerevisiae. Chlamydomonas reinhardtii Terpenoids C. reinhardtii is resistant to singlet oxygen, while S .cerevisiae is not. Probably due to different levels of carotenoid (terpenoids) biosynthesis. biosynthesis  [28,29] Kluyveromy ces lactis Production of different trepenoids from the same substrate has a flux and a time course similar to that in S. cerevisiae. Chlamydomonas reinhardtii C. reinhardtii is resistent to singlet oxygen, while S.cerevisiae is not. Probably due to different levels of carotenoid (terpenoids) biosynthesis. Supplementary Table 4 continued. One-carbon pool in cytoplasm required for synthesis of One carbon purines, pool by folate thymidylate and regeneration of methionine. [24,25,26]  Steroid biosynthesis Hsp70/Hsp90 organizing protein coordinates interactions during assembly of steroid receptor complexes. Supports maturation of Glucocorticoid receptor (GR) function.  Rattus norvegicus Kluyveromy ces lactis Homo sapiens One-carbon pool in cytoplasm required for synthesis of purines, thymidylate and regeneration of methionine. Time course and flux amount for Ergosterol and phytate production is the same between S. cerevisiae and K. lactis. Hsp70/Hsp90 organizing protein coordin ates interactions during assembly of steroid receptor complexes. Supports maturation of Glucocorticoid receptor (GR) function. Arabidopsis thaliana Drosophila melanogaster One-carbon pool in cytoplasm is only requiring for regeneration of methylation. It is not required for purine synthesis. Hsp70/Hsp90 organizing protein coordinates interactions during assembly of steroid receptor complexes. Does not support maturation of Glucocorticoid receptor (GR) function. Supplementary Table 4: A comparison of dynamic and adaptive responses of different organisms with S. cerevisiae. 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