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• Double helix -- twisted ladder shape of DNA, like spiral staircase
• Double helix -- twisted ladder shape of DNA, like spiral staircase

... one has 2nd strand filled in with matching nucleotides • Gene expression -- going from DNA to RNA to protein which results in phenotype, how the genotype determines the phenotype • Template -- model/pattern/stencil that makes copying easy and exact • Nucleotide -- basic unit of DNA, phosphate + suga ...
Gel Electrophoresis DNA Fingerprinting
Gel Electrophoresis DNA Fingerprinting

... • In this hypothetical case, DNA was extracted from samples obtained from the five possible suspects, and the crime scene sample • You will cleave the DNA with a restriction enzyme and simulated a “mock” DNA fingerprint analysis using Southern Blotting ...
Mini lab 11.1 and 11.2
Mini lab 11.1 and 11.2

... instructions into proteins requires a series of coordinated steps in transcription and translation. Procedure: 1. Use the data table below. Complete column B by writing the correct mRNA codon for each sequence of DNA bases listed in the column marked DNA Base Sequence. Use the letters A, U, C, G. 2. ...
Section 1.1 Name:
Section 1.1 Name:

... Review of Old Information: Recall that the DNA is the hereditary information for all living things. In this molecule is the code for all of our traits. However, one important question remains… how do we get from the genetic code from DNA in the nucleus, to the production of our phenotypes (or what w ...
Jatropha genotyping In Gh Pu QR In Gh Pu QR 13 primer pairs
Jatropha genotyping In Gh Pu QR In Gh Pu QR 13 primer pairs

Genetic Technology
Genetic Technology

... animals with human diseases and animals that can produce human materials. ...
History of Genetics
History of Genetics

... • Mutations, which are any change in the DNA base sequence), occur constantly in all cells and organisms. Offspring rarely get a perfect copy of the DNA from its parents. • but mutations are rare: about 1 DNA base change per 109 bases each cell generation. (Humans have about 3 x 109 bases and E. col ...
Given the following two evolutionary conserved eukaryotic genes A
Given the following two evolutionary conserved eukaryotic genes A

Proein Synthesis Note Fill-in
Proein Synthesis Note Fill-in

DNA etcTest Rev 07
DNA etcTest Rev 07

... The four N-bases in DNA are thymine, adenine, guanine, & cytosine. A hydrogen bond is found between the N-bases in DNA. Thymine always bonds with adenine in DNA, & cytosine with guanine due to complementary base pairing. 10. A section of DNA that codes for a protein is a(n) gene. 11. Chargaff’s rule ...
Francis Crick - WordPress.com
Francis Crick - WordPress.com

1.3. Identity: Molecules and Cells Study Guide (Fisher)
1.3. Identity: Molecules and Cells Study Guide (Fisher)

... and it is made up of building blocks of nucleic acids (just like RNA). It is a double-stranded helical molecule that the chromosomes in the nucleus of our cells are made of. DNA makes up genes, which make up chromosomes. Each gene codes for a protein (like hemoglobin), and each protein determines a ...
1.3. Identity: Molecules and Cells Study Guide
1.3. Identity: Molecules and Cells Study Guide

... and it is made up of building blocks of nucleic acids (just like RNA). It is a double-stranded helical molecule that the chromosomes in the nucleus of our cells are made of. DNA makes up genes, which make up chromosomes. Each gene codes for a protein (like hemoglobin), and each protein determines a ...
Genomic Organization in Eukaryotes
Genomic Organization in Eukaryotes

... • Addition of methyl groups (-CH3) to bases of DNA… • Many plants and animals do this, and it seems to be long-term control of gene expression. • In eukaryotes, genes that are not expressed (like Barr bodies) are more heavily methylated • Methylation ensures that once gene is turned off, it stays of ...
Name: Date: Per:______ DNA Guided Reading There are two types
Name: Date: Per:______ DNA Guided Reading There are two types

... nucleic acids are called nucleotides, which are made up of a phosphate group, a nitrogenous base (adenine, thymine, cytosine, guanine, and uracil), and a five carbon sugar. DNA provides the information to the cell for making all the protein the cell needs. Proteins are made of amino acids. The DNA h ...
File
File

... A-T; G-C). No base can join with itself (i.e. No A-A / T-T / G-G / C-C). ...
Chapter 1 The Framework of Biology
Chapter 1 The Framework of Biology

... twisted ladder. The sides of the ladder are the sugar and phosphate units, one side running in a 3' to 5' bonding arrangement, the other arranged 5' to 3'. The rungs of the ladder are matched bases: adenine to thymine or cystosine to guanine. The DNA in cells combines with proteins to form chromosom ...
Chapter 16 Quiz - Home - Union Academy Charter School
Chapter 16 Quiz - Home - Union Academy Charter School

... c. Chloroplast d. ribosome ...
Table 3.
Table 3.

... Low PCR yield Optimize PCR to enhance product yield. Optimize PCR conditions to obtain clean product or design new primers without secondary structures. ...
Lecture 18
Lecture 18

... cDNA We need a copy of the gene, but with the introns removed. Copy the mRNA instead of the gene. - reverse transcriptase ...
Genetic Engineering
Genetic Engineering

... pancreas of cows and pigs (limited production) • Today, most human insulin comes from human insulin-making genes transferred into simple cells such as bacteria or baker’s yeast (unlimited supply) – Identical to insulin made by the human pancreas ...
Quiz 3 review sheet
Quiz 3 review sheet

... cue,  show  how  DNA  polymerase  moves.   • Transcribe  RNA  strands  from  DNA  template,  being  able  to  identify  this  strand   by  the  location  of  the  promoter  and  the  directionality  of  each  strand.           • Use ...
30. genetic disorders 31. pedigree 32. Punnett Square
30. genetic disorders 31. pedigree 32. Punnett Square

... - a permanent change in a cell’s DNA, examples: deletion, insertions, duplication ...
Lecture 18
Lecture 18

... cDNA We need a copy of the gene, but with the introns removed. Copy the mRNA instead of the gene. - reverse transcriptase ...
Ch 16 Genetics Review
Ch 16 Genetics Review

... genetic code. • These chemicals act as the cell's memory, instructing it on how to synthesize enzymes and other proteins. These four nucleotides encode everything an organism needs to live and protects this information with incredible accuracy. ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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