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PreAP Biology Study Guide Unit 4: Molecular Genetics 4.1 What are
PreAP Biology Study Guide Unit 4: Molecular Genetics 4.1 What are

... What is the product/purpose of transcription? What is the product/purpose of translation? On which end, 5’ or 3’, of the DNA template strand is mRNA made? How are amino acid sequences synthesized? What happens when a ribosome is translocating? What happens at the A, P, and E site of the ribosome? Wh ...
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S-strain (virulent)

... These results led Griffith to believe that some material from the S-strain was transferred to the R-strain, which converted the R-strain to s-strain. Transformation - the process during which bacteria are changed by absorbing genetic material from an outside source. Griffith was still not sure whet ...
1.3. Identity: Molecules and Cells Study Guide
1.3. Identity: Molecules and Cells Study Guide

... 1.3.d How can tools of molecular biology be used to compare the DNA of two individuals? DNA can be extracted from a person & then scientists can perform PCR (polymerase chain reactions) to amplify the DNA, making a sample millions of times bigger than the original sample. They can then cut the DNA w ...
History of Genetics
History of Genetics

... • 1972: Stanley Cohen and Herbert Boyer combine DNA from two different species in vitro, then transform it into bacterial cells: first DNA cloning. • 2001: Sequence of the entire human genome is announced. ...
Regulation and Expression of Aldehyde Dehydrogenase in Normal
Regulation and Expression of Aldehyde Dehydrogenase in Normal

... Regulation and Expression of Aldehyde Dehydrogenase in Normal & Malignant Cells Summary of Project: Cancer is now recognised as a disease associated with both genetic and epigenetic changes. Aberrant changes of DNA methylation, histone modification and chromatin compartments are commonly associated ...
DNA Word Messages
DNA Word Messages

... 3. What is the process of transcription? 4. What location does transcription occur? 5. What is the process of translation? 6. What location does translation occur? 7. Each mRNA has a cap and poly-A-tail. What is their purpose? 8. Compare and contrast DNA polymerase and RNA polymerase? 9. Does transc ...
Cell Transformation
Cell Transformation

... segments of DNA. Characteristics produced by the segments of DNA may be expressed when these segments are inserted into new organisms, such as bacteria. Inserting, deleting, or substituting DNA segments can alter genes. (mutations) An altered gene may be passed on to every cell that develops from it ...
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[Type the document title] Microbial Genetics Molecular biology is the

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USE of direct amelogenin gene PCR for sex determination in

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... 1) Must allow for faithful replication - each strand of DNA serves as a template for replication 2) Must have information content - the sequence of bases predict the sequence of amino acids in proteins 3) Must be able to change in order to explain mutations changes in DNA sequences result in changes ...
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dna testing workshop 2005
dna testing workshop 2005

... Please briefly type or neatly print your answers to the following questions on separate pages and hand in by Friday Dec. 2, 2005. Questions are based on the DNA workshop, the heredity handout and reserve readings, but feel free to consult any other sources you wish, as long as you cite them. 1. Cons ...
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DNA quantification

... • Concentration and quality of a sample of DNA or RNA are measured with a UV spectrophotometer. • Since nitrogenous bases absorb UV light, the more concentrated the DNA solution, the more UV light it will absorb. • A solution containing 50 µg per ml of double strand DNA has an absorbancy (optical de ...
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Cell-free fetal DNA

Cell-free fetal DNA (cffDNA) is fetal DNA circulating freely in the maternal blood stream. It can be sampled by venipuncture on the mother. Analysis of cffDNA provides a method of non-invasive prenatal diagnosis.cffDNA originates from the trophoblasts making up the placenta. It is estimated that 2-6% of the DNA in the maternal blood is fetal in origin. The fetal DNA is fragmented and makes its way into the maternal bloodstream via shedding of the placental microparticles into the maternal bloodstream (figure 1). Studies have shown that cffDNA can first be observed as early as 7 weeks gestation, and the amount of cffDNA increases as the pregnancy progresses. cffDNA diminishes quickly after the birth of the baby, so that it is no longer detectable in the maternal blood approximately 2 hours after birth. cffDNA is significantly smaller than the maternal DNA in the bloodstream, with fragments approximately 200bp in size. Many protocols to extract the fetal DNA from the maternal plasma use its size to distinguish it from the maternal DNA.Studies have looked at, and some even optimized, protocols for testing non-compatible RhD factors, sex determination for X-linked genetic disorders and testing for single gene disorders. Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than the current prenatal testing methods, and have no risk of spontaneous abortion, unlike current prenatal testing methods. Non-invasive prenatal diagnosis (NIPD) has been implemented in the UK and parts of the US; it has clear benefits above the standard tests of chorionic villi sample (CVS) and amniocentesis which have procedure-related miscarriage risks of about 1 in 100 pregnancies and 1 in 200 pregnancies, respectively.As a method of prenatal diagnosis, cell-free fetal DNA techniques share the same ethical and practical issues, such as the possibility of prenatal sex discernment and sex selection.
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