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DNA Structure and Replication
DNA Structure and Replication

... expressed, interrupt most eukaryotic genes • Exons = portions of a gene that are expressed ...
Slide 1
Slide 1

... Individual genes of DNA can be copied into mRNA. All DNA on a chromosome is copied before the cell divides. Now instead of one pair (times 23) of chromosomes, we have two pairs (times 23). 1) The chromosomes are copied. 2) The cell’s nuclear membrane disappears. 3) Two organelles called centrioles m ...
dna-student - WordPress.com
dna-student - WordPress.com

... Humans have _______ chromosomes (23 pairs). One chromosome from each pair comes from your _________ and the other from your __________. Each chromosome carries the same genes but the information on the genes may be slightly __________, therefore you are a combination of the genes of both your parent ...
Re-closing linearized plasmids
Re-closing linearized plasmids

... Sequencing: • Select 1 or 2 of the correct plasmid clones and measure the DNA concentration using the Nanodrop. • Send 500-800 ng of each sample to Genewiz for sequencing. Ask Owen for the correct PO number to include in the paperwork. ...
SNC2D Genes - Malvern Science
SNC2D Genes - Malvern Science

... • Humans have 46 chromosomes in each somatic (body) cell (except the gametes = ovum/sperm, which have 23) • Different species may have different numbers of chromosomes ...
three possibile models for replication
three possibile models for replication

... 16) They can cause the release of digestive (hydrolytic) enzymes from lysosomes, which break down the host cell and eventually kill it 17) They can cause infected cells to produce toxins that lead to disease symptoms 18) Vaccines = harmless derivatives of viruses that stimulate the immune system to ...
Systematic Implications of DNA variation in subfamily Opuntioideae
Systematic Implications of DNA variation in subfamily Opuntioideae

Chapter 9 answers
Chapter 9 answers

... What would happen if all the genes in a cell were always active? It would take a huge amount of materials and energy in order to make all the proteins. Most of them would not be needed, and would take up space in the cell, or else they would need to be broken down again so the raw materials could be ...
2015 Test 3 study guide Bio 105
2015 Test 3 study guide Bio 105

... • Base pair rules • DNA is double helix and each strand is complementary • DNA strands held together by hydrogen bonds • 6.2 DNA replication • Method of duplication is semi-conservative • Replication occurs in the nucleus • Different organisms have some DNA sequences in common, the more closely rela ...
Recombinant and Synthetic Nucleic Acid Activity Registration
Recombinant and Synthetic Nucleic Acid Activity Registration

... Are not in organisms or viruses. Consist entirely of DNA segments from a single nonchromosomal or viral DNA source, though one or more of the segments may be a synthetic equivalent. Consist entirely of DNA from a prokaryotic host including its indigenous plasmids or viruses when propagated only in t ...
genome433
genome433

... microsatellite marker. A microsatellite is an STS which contains a tandem repeat of a very simple DNA sequence, e.g., (CA)n. Because errors are made in replicating such sequences the “n” often varies from one individual to another (i.e., it is polymorphic.) ...
Zoo/Bot 3333
Zoo/Bot 3333

... For answers to the quiz, click here: 1. The pufferfish, Fugu has a relatively small genome for a vertebrate, with a haploid genome size of about 380 Mb. If this genome is digested with AvaII (an enzyme with the recognition sequence: 5’-GG(A or T)CC-3’ approximately how many DNA fragments would be pr ...
Unit 10 Biotechnology review guide 2014
Unit 10 Biotechnology review guide 2014

LEQ: How do we splice new genes into DNA?
LEQ: How do we splice new genes into DNA?

... end of the gel; apply current; negative DNA is pulled to the positive end; smaller pieces travel farther faster; DNA fragments are separated based on the size of the fragment (# of base pairs). ...
All life is based on the same genetic code
All life is based on the same genetic code

... Each form of a gene is an allele. The standard (wild type) and altered (mutant) forms of the gene associated with hemoglobin and sickle cell anemia provide an example. The DNA sequences of both alleles of the “hemoglobin gene” are 99.9% identical – a single nucleotide difference makes for a single a ...
Gene Expression (Epigenetics)
Gene Expression (Epigenetics)

Name
Name

... Hybrid Vigor – hybrids that are hardier than either of their parents ...
DNA
DNA

... “A predatory bird” ...
Slide 1
Slide 1

... purebred red is crossed with a purebred white cow and results in a cow that is roan in colorhas both red and white hair) ...
Transformation laboratory
Transformation laboratory

... Laboratory: Bacterial Transformation Introduction of plasmid DNA into ...
NOTES: 12.1 - History of DNA (powerpoint)
NOTES: 12.1 - History of DNA (powerpoint)

... ● Heat killed pathogenic bacteria had passed their disease-causing ability to the harmless strain ● Griffith called this TRANSFORMATION – One strain of bacteria (harmless) had changed into the other (harmful, or disease-causing) ● Some factor was transferred from the heat killed cells to the live ce ...
12.1 - DNA History / Discovery
12.1 - DNA History / Discovery

... ● Heat killed pathogenic bacteria had passed their disease-causing ability to the harmless strain ● Griffith called this TRANSFORMATION – One strain of bacteria (harmless) had changed into the other (harmful, or disease-causing) ● Some factor was transferred from the heat killed cells to the live ce ...
Final Exam Review - Blue Valley Schools
Final Exam Review - Blue Valley Schools

GENETIC TRANSFER AND RECOMBINATION (Chapter 8):
GENETIC TRANSFER AND RECOMBINATION (Chapter 8):

... 2. Conjugation cells must be of opposite mating types (Donor cell carries plasmid) In Gram negative bacteria use a sex pili (projection of donor cell surface that contacts recipient and brings into contact) F factor (fertility factor): F+ cells have F plasmid/F- lack F plasmid F plasmid can integrat ...
Human Genomics - Mrs Smith`s Biology
Human Genomics - Mrs Smith`s Biology

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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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