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Canadian journal of Microbiology
Canadian journal of Microbiology

... acid and 2-hydroxyphenazine. We report here that PA23 produces additional compounds, including protease, lipase, hydrogen cyanide, and siderophores, that may contribute to its biocontrol ability. In the gacS mutant background, generation of these products was markedly reduced or delayed with the exc ...
Cancer Prone Disease Section Retinoblastoma Atlas of Genetics and Cytogenetics
Cancer Prone Disease Section Retinoblastoma Atlas of Genetics and Cytogenetics

... typically, these mutant alleles are associated with almost complete penetrance (>95%) and high expressivity (more than 6 individual retinoblastoma foci per individual and, therefore, most often involvement of both eyes); some rare mutant alleles that code for proteins with retention of parts of the ...
Identifying the genomic determinants of aging and longevity in
Identifying the genomic determinants of aging and longevity in

... by many genes with small effects it could be a successful method to identify novel longevity loci. However, so far, GWAS for longevity in the LLS [37], Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) [38, 39], NECS [40], German long-lived individuals [41], and SICS [42] have on ...
Journal of Bacteriology
Journal of Bacteriology

... day length was 16 h. The roots were not shielded from light, and no forced aeration was applied (41). Prior to cocultivation, bacteria were grown at 28°C on solid YMB medium (26) for 3 days. The cells were suspended from the plate in deposit-free Jensen medium to an A6. value of 0.1 and diluted 1,00 ...
A PCR approach to determine the distribution of toxin genes in
A PCR approach to determine the distribution of toxin genes in

... of wound and soft tissue infections in humans, classically war wounds, and in farm animals, but the species has been rarely encountered in clinical medicine over the past 50 years (Poxton, 2005). However, recently, awareness of these organisms has increased: C. novyi has been encountered in lethal i ...
Functional Genomics
Functional Genomics

... about by the avalanche of DNA sequence information. Vukmirovic and Tilghman provide an overview to the genomic revolution on page 820 and discuss what it will mean to scientists interested in the fundamentals of life. The progression of biology into a data-rich science has been orchestrated by compu ...
CHAPTER 7 TUNING THE DRAGON PROMOTER FINDER SYSTEM FOR HUMAN PROMOTER RECOGNITION
CHAPTER 7 TUNING THE DRAGON PROMOTER FINDER SYSTEM FOR HUMAN PROMOTER RECOGNITION

... short DNA segments around TSS representing the promoter data are separated into the G+C-rich and G+C-poor groups. This separation of data and subsequent development of models for both of these promoter groups, as well as the sophisticated tuning of the models, has resulted in considerably enhanced s ...
Fitness landscapes of gene regulation in variable - UvA-DARE
Fitness landscapes of gene regulation in variable - UvA-DARE

... ll living organisms are equipped with mechanisms that enable them to sense their environment and respond to it. In many cases the response consists of regulating gene expression. For bacteria the link between sensing and response is often formed by a small network of interacting proteins and regulat ...
Gene Section LPP (lipoma preferred partner) Atlas of Genetics and Cytogenetics
Gene Section LPP (lipoma preferred partner) Atlas of Genetics and Cytogenetics

... Because of their structural features (many proteinprotein interaction domains) and their characteristic to shuttle between the nucleus and the cytoplasm, LPP and its family members (see below) have been proposed to be scaffolding proteins involved in signal transduction from sites of cell adhesion t ...
Anterior boundaries of Hox gene expression in mesoderm
Anterior boundaries of Hox gene expression in mesoderm

... gene may encode positional information along the rostralcaudal axis [9]. Transcripts of the Hox 1.5 gene are expressed in mesoderm and ectoderm of 7- to 9-day p.c. embryos, but are spatially restricted to the ectoderm-derived neural tube, myencephalon, dorsal root ganglia and many mesodermderived ti ...
Promega Notes 49: Firefly Luciferase Engineered for Improved
Promega Notes 49: Firefly Luciferase Engineered for Improved

... sequences for transcription factor binding sites (5) and removed many sites which could potentially interact with common factors. In some cases where it was convenient in our modification strategy, we also removed less common potential regulatory sites. As with the removal of restriction sites, the ...
Chapter 4: EXTENSIONS OF MENDELIAN INHERITANCE
Chapter 4: EXTENSIONS OF MENDELIAN INHERITANCE

... Though dominant mutant alleles are much less common than recessive alleles, they do occur in natural populations. How can a mutant allele be dominant over a wild-type allele? Three explanations account for most dominant mutant alleles: a gainof-function mutation, a dominant-negative mutation, or hap ...
20060710_GOannotCamp_Stanford
20060710_GOannotCamp_Stanford

...  GO provides the set of reference genome (RG) annotations: 9 genomes, 9 organization of which E.coli Hub is the newest, selected for various reasons  Aim for ‘fully curated’ genomes, broad and deep annotation What does broad and deep annotation mean?  Broad: Every gene has a functional annotation ...
Scientific background report AMFLORA potato
Scientific background report AMFLORA potato

... In normal circumstances, night frost will kill off quite some of the potatoes unintentionally left behind on the field (by falling off the wagon, for example). Potatoes cannot withstand temperatures below -3 oC. Only a limited fraction of the potatoes left behind emerge in the next crop as volunteer ...
Three epigenetic information channels and their different roles in
Three epigenetic information channels and their different roles in

... emergence of complex multicellular organisms. Other authors have observed that in multicellular organisms, epigenetic marks will only be transmitted between generations provided they do not interfere with somatic differentiation and cell heredity (Jablonka & Lamb, 2005, pp. 148–150). Our contributio ...
5 Genetic Analysis of Kidney Disease in Mice
5 Genetic Analysis of Kidney Disease in Mice

... examples from several phenotypes. We begin by selecting QTL found in three or more crosses that also had a concordant human QTL. We first narrowed the region by comparative genomics if possible and then by statistically combining the crosses. Next, we used a dense SNP database to reduce the regions ...
Cloning of the Papaya Chromoplast-Specific
Cloning of the Papaya Chromoplast-Specific

... and the CYC-b cDNA probe on a single contig indicated that the papaya CYC-b orthologous gene was a strong candidate for controlling fruit flesh color. The papaya draft genome sequence of SunUp female integrated with FPC-1648 was subjected to Genescan, which identified a gene with homology to citrus ...
Alu Human Polymorphism
Alu Human Polymorphism

... inherited from each parent – Most occurred millions of years ago and are often on both pairs of chromosomes – There are Alu elements that have occurred since humans branched from other primates – This gives rise to dimorphic Alus from the last hundreds of thousands of years – Estimated to jump 1:200 ...
Green Fluorescent Protein-Glucocorticoid Receptor Knockin Mice
Green Fluorescent Protein-Glucocorticoid Receptor Knockin Mice

... response to GCs. It has been observed that both overexpression of GR and expression of antisense GR mRNA in transgenic mice alters thymocyte survival in vitro and in vivo (9 –11). Furthermore, the relative ratio of GR to other transcription factors within a given cell type determines whether the pre ...
Left-right axis asymmetry determining human Cryptic gene is
Left-right axis asymmetry determining human Cryptic gene is

... the need to identify the very initial events and genes that are regulated by Snail leading to asymmetric positioning of the organs. As part of the Nodal signalling, the EGF-CFC family [comprising of Cripto, Frl1 and Cryptic (CFC1)], is emerging as an important determinant of the body axes [9, 10]. H ...
The universal reagent for genome tailoring
The universal reagent for genome tailoring

... control that the remaining loxP site does not create a disturbance in the gene function after the selectable marker is removed. Interestingly, this allele without the marker removal (Fig. 3B) can provide a useful tool for learning more about the function of the gene of interest. If the marker was de ...
In vivo evidence for the prokaryotic model of extended codon
In vivo evidence for the prokaryotic model of extended codon

... trnfM gene does not affect the aminoacylation level, which remains at or near 100%. Similarly, lanes 2 and 3 of Figure 2B show that in the U-AUG+U strain, the mutant tRNA is fully aminoacylated. The chloroplast tRNALeu was used in all cases as a control for successful retention or removal of the cha ...
Monitoring BCR-ABL transcript levels by real-time
Monitoring BCR-ABL transcript levels by real-time

... method for monitoring the response to treatment in patients with chronic myeloid leukemia (CML) who have been induced into complete cytogenetic remission.1 Recently, various methods based on RQ-PCR have been adopted to monitor residual disease in clinical studies. An attempt to standardize this meth ...


... 1A). Members of the PR4 family of PR proteins, the class I and II PR4 chitinases, all have an intron at this position. The assignment of the transcription start site, which locates 81 nt upstream of the translation start site, was made by taking into account the longest ZmPR4 cDNA sequence previousl ...
"Frontmatter". In: Plant Genomics and Proteomics
"Frontmatter". In: Plant Genomics and Proteomics

... species. Therefore, it is harder to define the nature of a typical plant genome because the contribution of additional DNA may have phenotypic effects independent of the actual sequences of DNA present, for example, the role of nuclear DNA content in the annual versus perennial life cycle. An added ...
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Epigenetics of diabetes Type 2

In recent years it has become apparent that the environment and underlying mechanisms affect gene expression and the genome outside of the central dogma of biology. It has been found that many Epigenetic mechanisms are involved in the regulation and expression of genes such as DNA methylation and chromatin remodeling. These epigenetic mechanisms are believed to be a contributing factor to pathological diseases such as Diabetes type II. An understanding of the epigenome of Diabetes patients may help to elucidate otherwise hidden causes of this disease.
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