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Identification of One BOCR Mutation and Five NF1 Mutations in Male
Identification of One BOCR Mutation and Five NF1 Mutations in Male

... In the six Chinese patients with NF1 and CPT, we identified two missense, one nonsense, and two deletion mutations in the NF1 gene. These mutations detected using high throughput sequencing method and validated by Sanger sequencing were rarely found in large sequencing databases of controls. Two of ...
IMPROVE SMALL RNA-MEDIATED GENE SILENCING
IMPROVE SMALL RNA-MEDIATED GENE SILENCING

... We now have a much greater understanding of the gene silencing pathways in plants and their crucial roles in a variety of biological regulation processes, such as development, plant defense against invading viral nucleic acids, and epigenetic modifications. siRNA directed target repression can occur ...
Gene Section EVI1 (ecotropic viral integration site 1 (EVI1) and
Gene Section EVI1 (ecotropic viral integration site 1 (EVI1) and

... Exon 3 of the human EVI1 gene contains two closely spaced ATG codons, either of which may serve as the translation initiation site. Depending on which ATG is used, proteins of 1051 or 1041 amino acids will be formed. EVI1 contains two domains of seven and three zinc finger motifs, respectively, a re ...
Tumor metastasis-associated human MTA1 gene and its MTA1
Tumor metastasis-associated human MTA1 gene and its MTA1

... (NuRD complex), which is involved in chromatin remodeling, contains MTA1 protein or a MTA1-related protein (MTA2) [16, 17]. Thus, a possible function for the MTA1 protein has been reported; however, the exact role of the MTA1 protein in tumor progression and metastasis must still be determined. Here ...
Insulin-like growth factor (IGF) signalling is required for early dorso
Insulin-like growth factor (IGF) signalling is required for early dorso

... To further characterise the phenotype of DN-IGF-1R expressing embryos, we examined the expression of a number of genes that are critical for normal neural development and for notochord and somite formation. We initially examined expression of Pax6.2, Six3.1, Rx3 and Otx2 in 10 hpf embryos. At 10 hpf ...
NanoString™: User Guide | nCounter® Expression Data Analysis
NanoString™: User Guide | nCounter® Expression Data Analysis

... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
Frequent, independent transfers of a catabolic gene from bacteria to
Frequent, independent transfers of a catabolic gene from bacteria to

... of a particular species can differ by large fractions of their genome [2–6]. Long thought to be a prokaryote specialty, HGT is now recognized as a mechanism of genetic innovation in eukaryotes as well [7–10]. Genome analysis of eukaryotes revealed that several genes had been horizontally transferred ...
Extensions and Modifications of Basic Principles
Extensions and Modifications of Basic Principles

... incomplete. The important thing to remember about dominance is that it affects the phenotype that genes produce, but not the way in which genes are inherited. Another type of interaction between alleles is codominance, in which the phenotype of the heterozygote is not intermediate between the phenot ...
Stress and Atopic Disorders: Asthma and the Seasonal
Stress and Atopic Disorders: Asthma and the Seasonal

... and asthma expression may also be influenced by cytokines produced through pathways or inflammatory processes not necessarily directly related to the TH1:Th2 dichotomy14. For example, some proteins are secreted both by Th1 and Th2 cells [e.g., tumor necrosis factor alpha (TNF-)]. Studies suggest th ...
Gene Signal Estimates from Exon Arrays v1.0
Gene Signal Estimates from Exon Arrays v1.0

... containing probe sets that interrogate exons of RefSeq (Pruitt and Maglott 2001) genes, mRNAs and ESTs from GenBank (Benson et al. 1999), it also contains probe sets for exons predicted by ab-initio gene finders such as GENSCAN (Burge and Karlin 1997), TWINSCAN (Korf et al. 2001), geneid (Parra et a ...
Reciprocal deletion and duplication at 2q23.1 indicates a
Reciprocal deletion and duplication at 2q23.1 indicates a

... INTRODUCTION Copy number variations (CNVs) have an important role in the genetic etiology of many neurodevelopmental disorders, including intellectual disability (ID) and autism. Studies show that neurodevelopmental syndromes associated with CNVs emerge from abnormal gene dosage, suggesting that str ...
A Complex Suite of Forces Drives Gene Traffic from Drosophila X
A Complex Suite of Forces Drives Gene Traffic from Drosophila X

... from both subgenera. A subset of these data was extracted consisting of lineage-specific duplications in which the family had only a single duplication event along a particular lineage (phylogenetic method—two copies). The second approach (counting method) took advantage of the finding that a maximu ...
The Ehlers-Danlos syndrome: on beyond collagens
The Ehlers-Danlos syndrome: on beyond collagens

... allow improved genetic counseling through correlation of mutant genotypes with specific outcomes or complications. However, as outlined below, the molecular defects described to date are not sufficient to explain disease in many EDS patients, including those with the most common classical and hyperm ...
The SELF-PRUNING gene of tomato regulates
The SELF-PRUNING gene of tomato regulates

... sympodial segment that was formed at the axil of the last leaf (leaf 11) is composed of an inflorescence and only one leaf (leaf L1). A more advanced sp mutant ‘determinate’ apex is shown in Fig. 3F. In terms of inflorescence structure, the first apical dome of the reproductive meristem is fated to ...
mtr function Background Luis M. Corrochano
mtr function Background Luis M. Corrochano

... spreading 0.2 ml of conidial suspension on the surface of the selective agar or by use of spreading agar with the appropriate supplements. The handling and plating of conidia should be performed in darkness or dim light to prevent photorepair. The plates should be incubated in the dark, at least for ...
Valpromide Inhibits Lytic Cycle Reactivation of Epstein
Valpromide Inhibits Lytic Cycle Reactivation of Epstein

... ABSTRACT Reactivation of Epstein-Barr virus (EBV) from latency into the lytic phase of its life cycle allows the virus to spread among cells and between hosts. Valproic acid (VPA) inhibits initiation of the lytic cycle in EBV-infected B lymphoma cells. While VPA blocks viral lytic gene expression, i ...
PDF
PDF

... patterns of gene expression in response to metabolic require- tumor-suppressor gene. We considered a first exon to be a genomic region bordered by ments, developmental programs and external stimuli17. The localization of these regulatory regions is important for under- a promoter region (5′ boundary ...
Liver Effects of Clinical Drugs Differentiated in Human Liver
Liver Effects of Clinical Drugs Differentiated in Human Liver

... methimazole (MMI, 500 µM), terbinafine (TBF, 100 µM), and carbamazepine (CBZ 100 µM). Oxidative stress genes were affected by TBF (18%), CBZ, APAP, and ETM (12%–11%), and mitochondrial genes were altered by CBZ, APAP, MMI, and ETM (8%–6%). Apoptosis genes were affected by DCF (14%), while apoptosis ...
Note for Guidance on the Quality, Preclinical and Clinical
Note for Guidance on the Quality, Preclinical and Clinical

... The design of the gene transfer product forms an integral part of the rationale for the development of a gene transfer medicinal product. Information relating to the suitability of the vector system and of the delivery system should be provided. Data on the control and stability of gene expression s ...
Classification of colorectal cancer based on correlation of clinical
Classification of colorectal cancer based on correlation of clinical

... MSI-H CRC.35 The finding of CTNNB1 mutation in earlyonset cases of MSI-H CRC29 could be due to either Lynch syndrome or germ-line hemi-allelic methylation of MLH1.36 The over-representation of CTNNB1 mutations in MSI-H CRC cell lines37 is probably due to the fact that very few such cell lines are de ...
Joseph L. Izzo, Jr Answer the Genetic Riddle Aging and Systolic
Joseph L. Izzo, Jr Answer the Genetic Riddle Aging and Systolic

... systolic blood pressure as the more important end point in diagnosis, risk stratification, and therapy of hypertension.3 The graphic representation of the complex relationship between aging and blood pressure in humans2 should be prominently featured on posters on the walls of genetic laboratories a ...
Lecture-Mic 623-Plasmids-Listeria - Home
Lecture-Mic 623-Plasmids-Listeria - Home

... differentiated 565 apparently unrelated cultures into four groups, the smallest being 5% of cultures resistant to both agents, and the largest (53%) being sensitive to cadmium and resistant to arsenic. The resistance patterns to these agents and the presence of plasmid DNA varied markedly between th ...
Hemophilia
Hemophilia

... of the disease may be diagnosed only when they accidentally have a severe injury. Tests are widely available for hemophilia. Since both hemophilia types (A and B) cause the same complications but are treated differently, distinguishing the type of hemophilia via special blood test is extremely impor ...
Category 2000
Category 2000

... (Cercopithecus aethiops). 20 monkeys were genotyped for polymorphisms in the genes encoding ATP-binding cassette transport A1 (ABCA1) and cholesteryl transfer ester protein (CETP). An average of 5 single nucleotide polymorphisms (R219K, V771M, V825I, I883M and I405V) was genotyped within these genes ...
Etiology : cytogenetics and microdeletions - HAL
Etiology : cytogenetics and microdeletions - HAL

... flanking a genomic rearrangement should be considered as possible contributors to the phenotype. In addition, the interpretation of these observations is not obvious, as most of the chromosomal aberrations are large. One strategy would be to use software dedicated to the prioritization of candidate ...
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Epigenetics of diabetes Type 2

In recent years it has become apparent that the environment and underlying mechanisms affect gene expression and the genome outside of the central dogma of biology. It has been found that many Epigenetic mechanisms are involved in the regulation and expression of genes such as DNA methylation and chromatin remodeling. These epigenetic mechanisms are believed to be a contributing factor to pathological diseases such as Diabetes type II. An understanding of the epigenome of Diabetes patients may help to elucidate otherwise hidden causes of this disease.
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