Insulin-like growth factor binding protein-2 (IGFBP
... IGFBP-2b mRNA were distinctly increased from 17 to 23 dph, but suddenly dropped to a lower level in and after 29 dph. However, the levels of IGFBP-2b mRNA during metamorphosis were greatly down-regulated after TU treatment. These results provided basic information for further studies on the role of ...
... IGFBP-2b mRNA were distinctly increased from 17 to 23 dph, but suddenly dropped to a lower level in and after 29 dph. However, the levels of IGFBP-2b mRNA during metamorphosis were greatly down-regulated after TU treatment. These results provided basic information for further studies on the role of ...
The murine homologue of HIRA, a DiGeorge
... Figure 1. (A) A scale schematic drawing of the assembled mouse Hira cDNA and the individual cDNA clones isolated from an E11.5 mouse embryo cDNA library (12F, 12B, 12E, 6A, 12D, 12C and 6B). As a reference the human and murine Tuple1 C5 and MF2 cDNAs and human HIRA are shown. Nucleotide positions ar ...
... Figure 1. (A) A scale schematic drawing of the assembled mouse Hira cDNA and the individual cDNA clones isolated from an E11.5 mouse embryo cDNA library (12F, 12B, 12E, 6A, 12D, 12C and 6B). As a reference the human and murine Tuple1 C5 and MF2 cDNAs and human HIRA are shown. Nucleotide positions ar ...
special - Microbiology
... To address this question, high-copy-number plasmids that contained either afsR or afsS were made and both were shown to be capable of stimulating Act production in both S. coelicolor and S.lividans; however, the degree of stimulation mediated by afxR was significantly greater than that produced by a ...
... To address this question, high-copy-number plasmids that contained either afsR or afsS were made and both were shown to be capable of stimulating Act production in both S. coelicolor and S.lividans; however, the degree of stimulation mediated by afxR was significantly greater than that produced by a ...
Transgenerational epigenetics in the germline cycle
... with heritable epigenetic states (for example, [5,6]). However, DNA methylation and its heritable maintenance in those organisms where it occurs is influenced, if not regulated, by histone modifying activities [7]. As will be discussed in this review, recent studies in C. elegans have shown that def ...
... with heritable epigenetic states (for example, [5,6]). However, DNA methylation and its heritable maintenance in those organisms where it occurs is influenced, if not regulated, by histone modifying activities [7]. As will be discussed in this review, recent studies in C. elegans have shown that def ...
Higher order structural effects stabilizing the
... give several ‘noncanonical’ H-bonding interactions, by either using the Watson–Crick edge in a reversed way or involving their ‘Sugar’ or ‘Hoogsteen’ edges (7–9). These noncanonical interactions offer a structural flexibility to folding strategies, and it is maybe not by chance that they are often in ...
... give several ‘noncanonical’ H-bonding interactions, by either using the Watson–Crick edge in a reversed way or involving their ‘Sugar’ or ‘Hoogsteen’ edges (7–9). These noncanonical interactions offer a structural flexibility to folding strategies, and it is maybe not by chance that they are often in ...
Construction and Characterization of a Highly Regulable Expression
... region and the lacUV5 ⫺10 region which contains a strong TATAAT site instead of the weak TATGTT site. The pET family of vectors uses the strong T7 promoter. Given this information, perhaps it is not surprising that researchers have found it is not possible to tightly shut off genes that are cloned i ...
... region and the lacUV5 ⫺10 region which contains a strong TATAAT site instead of the weak TATGTT site. The pET family of vectors uses the strong T7 promoter. Given this information, perhaps it is not surprising that researchers have found it is not possible to tightly shut off genes that are cloned i ...
Transvection and pairing of a Drosophila Hox long noncoding RNA
... 1995). This region is also associated with numerous mutations resulting in a dominant gain of function (GOF) Scr homeotic transformation, whereby sex combs (normally specific to the male T1 leg) are ectopically formed on legs of the T2 and T3 segments (Pattatucci et al. 1991). Strikingly, five diffe ...
... 1995). This region is also associated with numerous mutations resulting in a dominant gain of function (GOF) Scr homeotic transformation, whereby sex combs (normally specific to the male T1 leg) are ectopically formed on legs of the T2 and T3 segments (Pattatucci et al. 1991). Strikingly, five diffe ...
Synthesis of Oligonucleotides
... Nucleobases. Permanent protecting groups for the exocyclic amino groups of adenine, cytosine and guanine have been used for many years in oligonucleotide synthesis.1 Acyl protecting groups were chosen, since they are stable for long periods during mildly basic and acidic conditions used during oligo ...
... Nucleobases. Permanent protecting groups for the exocyclic amino groups of adenine, cytosine and guanine have been used for many years in oligonucleotide synthesis.1 Acyl protecting groups were chosen, since they are stable for long periods during mildly basic and acidic conditions used during oligo ...
Origins and Early Evolution of the tRNA Molecule
... Francis Crick once remarked that transfer RNA (tRNA) looks like nature’s attempt to make RNA do the job of a protein [1]. tRNA, discovered by Paul Zamecnik and collaborators [2], is a literal “adaptor” molecule [3] that mediates the translation of information from messenger RNAs (mRNAs). tRNA was th ...
... Francis Crick once remarked that transfer RNA (tRNA) looks like nature’s attempt to make RNA do the job of a protein [1]. tRNA, discovered by Paul Zamecnik and collaborators [2], is a literal “adaptor” molecule [3] that mediates the translation of information from messenger RNAs (mRNAs). tRNA was th ...
FS406-22
... and 95°C (Fig. 1). Agitation of the culture was nec(12). Cluster 2 includes molybdenum dinitrogenase reductases from methanogens, as well as essary for growth on N2, and clusters of two or more alternative vanadium and iron-only dinitrogenase reductases (VnfH, AnfH) from Methanosarcinales cocci were ...
... and 95°C (Fig. 1). Agitation of the culture was nec(12). Cluster 2 includes molybdenum dinitrogenase reductases from methanogens, as well as essary for growth on N2, and clusters of two or more alternative vanadium and iron-only dinitrogenase reductases (VnfH, AnfH) from Methanosarcinales cocci were ...
The biased nucleotide composition of the HIV genome: a constant
... (LTRs) are available for these groups (Table 1). As the LTR is relatively A-poor [5], calculations based upon the coding regions only will result in higher A-levels. Interestingly, HIV-1 group M subtypes A, B, C and D have significantly different nucleotide compositions concerning the A- and G-perce ...
... (LTRs) are available for these groups (Table 1). As the LTR is relatively A-poor [5], calculations based upon the coding regions only will result in higher A-levels. Interestingly, HIV-1 group M subtypes A, B, C and D have significantly different nucleotide compositions concerning the A- and G-perce ...
Two enhancer regions in the mouse En-2 locus
... et al., 1991). In the adult, En-2 is strongly expressed in cells of the internal granular layer of the cerebellum. No expression is detected in the neighbouring Purkinje cells, whereas weak expression is detectable in cells of the molecular layer (Davis et al., 1988; Davis and Joyner, 1988; K. Mille ...
... et al., 1991). In the adult, En-2 is strongly expressed in cells of the internal granular layer of the cerebellum. No expression is detected in the neighbouring Purkinje cells, whereas weak expression is detectable in cells of the molecular layer (Davis et al., 1988; Davis and Joyner, 1988; K. Mille ...
Recognition of Nucleic Acid Bases and Base
... donates two hydrogen bonds to the O6 and N7 acceptor groups of guanine and a second in which the carboxamide group of Asn (or Gln) hydrogen bonds to the N7 acceptor and N6 donor groups of adenine. These interactions are indeed the most commonly observed in protein –DNA complexes,4,7 – 9 and the impo ...
... donates two hydrogen bonds to the O6 and N7 acceptor groups of guanine and a second in which the carboxamide group of Asn (or Gln) hydrogen bonds to the N7 acceptor and N6 donor groups of adenine. These interactions are indeed the most commonly observed in protein –DNA complexes,4,7 – 9 and the impo ...
A rule-based kinetic model of RNA polymerase II C
... adopt [3] are simulated stochastically, and thus the approach accounts for low molecule numbers and the discrete states that molecules can adopt. We discuss transcription by RNA polymerase II (RNAPII) as an example of a complex system that can be modelled in detail using a rule-based approach. We pr ...
... adopt [3] are simulated stochastically, and thus the approach accounts for low molecule numbers and the discrete states that molecules can adopt. We discuss transcription by RNA polymerase II (RNAPII) as an example of a complex system that can be modelled in detail using a rule-based approach. We pr ...
and GvpD-mediated transcription regulation of the p
... sequence; primer pair used was pA-XbaI, 59-CTCCGTATCTAGAAGTACGAC-39 and 39-pgvpA-BamHI, 59-CGTCAGCGTAGGATCCGAACTCCTGCTG-39; restriction sites are shown in italics). The O construct contained a 483 bp XbaI–BamHI p-gvpO fragment (position 2592–3074 in X64729; primer pair used was pO-XbaI, 59-GCGCGAAGA ...
... sequence; primer pair used was pA-XbaI, 59-CTCCGTATCTAGAAGTACGAC-39 and 39-pgvpA-BamHI, 59-CGTCAGCGTAGGATCCGAACTCCTGCTG-39; restriction sites are shown in italics). The O construct contained a 483 bp XbaI–BamHI p-gvpO fragment (position 2592–3074 in X64729; primer pair used was pO-XbaI, 59-GCGCGAAGA ...
Fig 16.12a - McGraw Hill Higher Education
... The Myc-Max mechanism can activate or repress transcription Myc − identified as an oncogene • Regulates transcription of genes involved in cell proliferation • Doesn't have DNA binding activity on its own Max − identified through its binding to Myc • Max/Max homodimers and Myc/Max heterodimers bind ...
... The Myc-Max mechanism can activate or repress transcription Myc − identified as an oncogene • Regulates transcription of genes involved in cell proliferation • Doesn't have DNA binding activity on its own Max − identified through its binding to Myc • Max/Max homodimers and Myc/Max heterodimers bind ...
Document
... responsible – regions adjacent to bright spots will gain the same fraction of light. A comparison of many images at different levels of blurriness will appear to indicate that dark regions adjacent to bright regions are correlated in their ...
... responsible – regions adjacent to bright spots will gain the same fraction of light. A comparison of many images at different levels of blurriness will appear to indicate that dark regions adjacent to bright regions are correlated in their ...
MicroRNA Regulatory Patterns on the Human Metabolic Network
... subgraph of human metabolic network contains 1,099 nodes, 4,169 links and represents over 70% of the nodes, nearly 90% of the links of the human network and most metabolic pathways including all the central pathways (Supplementary Data 2). Therefore, we took this largest subgraph as the network in t ...
... subgraph of human metabolic network contains 1,099 nodes, 4,169 links and represents over 70% of the nodes, nearly 90% of the links of the human network and most metabolic pathways including all the central pathways (Supplementary Data 2). Therefore, we took this largest subgraph as the network in t ...
Functions of the nonsense-mediated RNA decay pathway in Drosophila development. PLoS Genetics 2 , 2143-2154.
... exon–exon boundary are generally recognized as premature and target the mRNA for destruction by NMD. Such boundaries are marked after splicing by deposition of a multiprotein complex, the exon junction complex (EJC), which includes Upf2 and Upf3. One current model proposes that EJCs along an mRNA ar ...
... exon–exon boundary are generally recognized as premature and target the mRNA for destruction by NMD. Such boundaries are marked after splicing by deposition of a multiprotein complex, the exon junction complex (EJC), which includes Upf2 and Upf3. One current model proposes that EJCs along an mRNA ar ...
development, the Linker histone H1 is essential for Drosophila
... functions of linker histones in vivo have used gene inactivation approaches. Elimination of the linker histone-like protein Hho1p in Saccharomyces cerevisiae did not cause any major phenotypic effects, nor were any perturbations in chromosome structure apparent (Ushinsky et al. 1997; Patterton et al ...
... functions of linker histones in vivo have used gene inactivation approaches. Elimination of the linker histone-like protein Hho1p in Saccharomyces cerevisiae did not cause any major phenotypic effects, nor were any perturbations in chromosome structure apparent (Ushinsky et al. 1997; Patterton et al ...