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Determining the Transcription Factor Genes Populating a Fruit Fly
Determining the Transcription Factor Genes Populating a Fruit Fly

... created when the RNAi screen caused other conspicuous phenotypes, possibly but not necessarily related to pigmentation (Rogers et al., 2014). Another important aspect of this research was the sexual dimorphism of the trait. We were interested in whether the transcription factors we found to be invol ...
Xenopus Spinal Neurons Express Kv2 Potassium Channel
Xenopus Spinal Neurons Express Kv2 Potassium Channel

... cells of the embryonic nervous system. In particular, Kvl.1 transcripts localize specifically to embryonic sensory neurons (Ribera and Nguyen, 1993). The restricted expression of Kvl.1 mRNA, however, suggests that additional genes account for the endogenous delayed rectifier potassium current of Xen ...
Real-time PCR Handbook
Real-time PCR Handbook

... specific sequences within a DNA or cDNA template can be copied, or “amplified”, many thousand- to a million-fold using sequence specific oligonucleotides, heat stable DNA polymerase, and thermal cycling. In traditional (endpoint) PCR, detection and quantification of the amplified sequence are perfor ...
Modified uridine at wobble position in tRNA of
Modified uridine at wobble position in tRNA of

... cytidine (C), and uridine (U). Although modified nucleosides are present at many positions in tRNAs, two positions in the anticodon region, position 34 (wobble position) and position 37, show the largest variety of modified nucleosides. In Saccharomyces cerevisiae, the xm5U type of modified uridines ...
A caudal mRNA gradient controls posterior development in the wasp
A caudal mRNA gradient controls posterior development in the wasp

MyGene.info Documentation
MyGene.info Documentation

... Optional, can be a comma-separated fields to limit the fields returned from the matching gene hits. The supported field names can be found from any gene object (e.g. gene 1017). Note that it supports dot notation as well, e.g., you can pass “refseq.rna”. If “fields=all”, all available fields will be ...
Document
Document

Recruitment of CRABS CLAW to promote nectary development
Recruitment of CRABS CLAW to promote nectary development

... perianth positions in basal taxa to central positions associated with reproductive organs in more derived taxa. In addition, extrafloral nectaries are currently known in 68 angiosperm families (Elias, 1983). Their structures and locations are also diverse across the families, although they occur mos ...
PDF
PDF

... the multiple conversion steps for synthesizing ecdysone from dietary cholesterol, the conversion of 7-dehydrocholesterol to 5ketodiol, the so-called ‘Black Box’, is thought to be the important rate-limiting step. Although a number of genes essential for ecdysone synthesis have recently been reveale ...
Function and Control of the Spx-Family of Proteins Within
Function and Control of the Spx-Family of Proteins Within

... (Buchko et al. 2011; Teplyakov et al. 2004). The fourth and largest clade is the “ArsC–ArsC” grouping that includes the bona fide arsenate reductase from E. coli and its orthologs from a variety of Gram-positive and -negative species. Mutations in a gene encoding an ArsC-like protein were reported i ...
ACTIN-RELATED PROTEIN6 Regulates Female Meiosis By
ACTIN-RELATED PROTEIN6 Regulates Female Meiosis By

... the SWR1 ATPase, enhance complex function (Mizuguchi et al., 2004). Homologs of all yeast and 11 human SWR1 subunits have been identified in Arabidopsis thaliana (March-Díaz and Reyes, 2009; Meagher et al., 2009), indicating that Arabidopsis has the SWR1 complex. Additionally, H2A.Z deposition at man ...
Stress Induction of Mitochondrial Formate Dehydrogenase in Potato
Stress Induction of Mitochondrial Formate Dehydrogenase in Potato

... which was subsequently identified in our laboratory as FDH. In this paper we describe the expression of potato mitochondrial FDH in various tissues and under several stresses. The mRNA levels of the mitochondrial SHMT isoform were studied in parallel because this enzyme was previously described to b ...
Mouse Lefty2 and Zebrafish Antivin Are Feedback
Mouse Lefty2 and Zebrafish Antivin Are Feedback

... and display a phenotype identical to sqt;cyc double mutants (Gritsman et al., 1999). These results indicate that Nodal signals not only require TGFb-like receptors, but are also dependent on extracellular cofactors of the EGF-CFC family. A novel family of TGFb molecules implicated in vertebrate embr ...
Partial report - GEP Community Server
Partial report - GEP Community Server

Cloning, Sequencing, and Characterization of Luciola italica
Cloning, Sequencing, and Characterization of Luciola italica

... from the firefly lanterns. The L. italica luciferase cDNA was successfully cloned by RT-PCR using a genespecific primer set based on the DNA sequence of the Eastern European Luciola mingrelica luciferase gene. The L. italica cDNA was determined to be 1647 base pairs in length with an open reading fr ...
Investigating the link between tRNA and mRNA - EMBL-EBI
Investigating the link between tRNA and mRNA - EMBL-EBI

... The genetic code describes how a sequence of codons on an mrna is translated into a sequence of amino acids, forming a protein. The genetic code manifests itself in the cell as trna molecules, which fall into several classes of anticodon isoacceptors, each decoding a single codon into its correspond ...
Introduction to Bioinformatics
Introduction to Bioinformatics

... melanogaster (Fruit Fly) ...
Computational Definition of
Computational Definition of

... constitutively spliced exons. We focused on constitutive splicing as opposed to alternative splicing so as to tackle the more fundamental problem represented by the former and to avoid what are likely more complex mechanisms in the latter. The strategy we used to overcome the confounding presence of ...
Multiple RNA regulatory elements mediate distinct
Multiple RNA regulatory elements mediate distinct

... Pattern formation in the early development of many organisms relies on localized cytoplasmic proteins, which can be prelocalized as mRNAs. The Drosophila oskar gene, required both for posterior body patterning and germ cell determination, encodes one such mRNA. Localization of oskar mRNA is an elabo ...
Inhibitors of HIV-1 Reverse Transcriptase—Associated
Inhibitors of HIV-1 Reverse Transcriptase—Associated

... end of the RNA genome, providing a strand of HIV (-) DNA complementary to the U5 and R terminal repeats of HIV genomic RNA. These newly synthesized sequences are essential for hybridization to WKH¶-end of the HIV genomic RNA template to enable completion of full length (-) DNA synthesis. However, ...
The enhancement of ribosomal transcription by the recycling of RNA
The enhancement of ribosomal transcription by the recycling of RNA

... contrast to the results of McStay and Reeder, (9), these effects were only seen when polymerase molecules from upstream initiation sites, transcribed through the pre-rRNA promoter. Grummt et al. (10) and Henderson and Sollner-Webb (11) interpreted these experiments in terms of Readthrough Enhancemen ...
Inhibitors of HIV-1 Reverse Transcriptase—Associated
Inhibitors of HIV-1 Reverse Transcriptase—Associated

... end of the RNA genome, providing a strand of HIV (-) DNA complementary to the U5 and R terminal repeats of HIV genomic RNA. These newly synthesized sequences are essential for hybridization to WKH¶-end of the HIV genomic RNA template to enable completion of full length (-) DNA synthesis. However, ...
Causes that Make a Difference - Philsci
Causes that Make a Difference - Philsci

PPT
PPT

... Strong, easy to detect, but rare in population; may not be reflective of common disease. Also, hard to collect family data. ...
Evidence from glycine transfer RNA of a frozen accident at the dawn
Evidence from glycine transfer RNA of a frozen accident at the dawn

... A37 has been replaced by G, perhaps because of a general increase in G+C content in the tRNA and rRNA of these species [18]. There are three other sequences not containing an anticodon loop CCA that appear to be isolated examples, but in these cases their origin is unclear. Schimmel's experimental w ...
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RNA silencing

RNA silencing (associated with the concept of post-transcriptional gene silencing or RNA interference) refers to a family of gene silencing effects by which the expression of one or more genes is downregulated or entirely suppressed by non-coding RNAs, particularly small RNAs. It may also refer to the introduction of a synthetic antisense RNA molecule used in scientific experiments on gene expression. RNA silencing may also be defined as sequence-specific regulation of gene expression triggered by double-stranded RNA (dsRNA). RNA silencing mechanisms are highly conserved in most eukaryotes. The most common and well-studied example is RNA interference (RNAi), in which endogenously expressed microRNA (miRNA) or exogenously derived small interfering RNA (siRNA) induces the degradation of complementary messenger RNA. Other classes of small RNA have been identified, including piwi-interacting RNA (piRNA) and its subspecies repeat associated small interfering RNA (rasiRNA).
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