lecture24_RnaInterfe.. - University of Alberta
... gene silencing phenomena; in 1998, Fire and Mello compared the silencing activity of singlestranded RNAs (ssRNAs) (sense or antisense) with double-stranded (dsRNAs) hybrids; marginal silencing was achieved by injecting C. elegans with ssRNAs, but potent and specific silencing was achieved by injecti ...
... gene silencing phenomena; in 1998, Fire and Mello compared the silencing activity of singlestranded RNAs (ssRNAs) (sense or antisense) with double-stranded (dsRNAs) hybrids; marginal silencing was achieved by injecting C. elegans with ssRNAs, but potent and specific silencing was achieved by injecti ...
Dicer-Like
... What is Dicer’s role in RNAi? • Activated by exogenous double-stranded (ds) RNA • miRNA (micro RNA) -small, non-coding regions of double-stranded (ds) RNA 21-22 nucleotides ...
... What is Dicer’s role in RNAi? • Activated by exogenous double-stranded (ds) RNA • miRNA (micro RNA) -small, non-coding regions of double-stranded (ds) RNA 21-22 nucleotides ...
PowerPoint
... Short RNAs regulate gene expression by different mechanisms, but use some of the same cellular machinery (e.g., Dicer) to do it. ...
... Short RNAs regulate gene expression by different mechanisms, but use some of the same cellular machinery (e.g., Dicer) to do it. ...
BIT 471/571:RNA Interference and Model Organisms
... • Introduce students to the history of RNA Interference (RNAi) and its applications in common model organisms with a focus on the experimental design • Students perform RNAi experiments in Nicotiana benthamiana (tobacco plants), in Caenorhabditis elegans (C. elegans) and in mammalian cell culture (h ...
... • Introduce students to the history of RNA Interference (RNAi) and its applications in common model organisms with a focus on the experimental design • Students perform RNAi experiments in Nicotiana benthamiana (tobacco plants), in Caenorhabditis elegans (C. elegans) and in mammalian cell culture (h ...