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BIO 103 - Genes
BIO 103 - Genes

... • introns – non-coding sequences • exons – sequences that will be translated ...
Eukaryotic Gene Expression ppt
Eukaryotic Gene Expression ppt

The RNA World
The RNA World

... bind target and prevent ribosomal elongation ...
The RNA World
The RNA World

... bind target and prevent ribosomal elongation ...
RNA interference - Creighton University
RNA interference - Creighton University

... silencing (PTGS), but can also effect transcriptional gene silencing (TGS) • RNAi is highly conserved among all eukaryotic organisms (fungi, protozoans, plants, nematodes, invertebrates, mammals) ...
Module Name Module Credit Value Module Coordinator Lecturers
Module Name Module Credit Value Module Coordinator Lecturers

... Students will be provided with primary research articles and scientific reviews on specialised research topics for self-guided study. Short Content Overview This module builds on the core concepts of bacterial and eukaryotic gene expression studied in the Junior Sophister year and introduces student ...
Model of Star‐PAP‐mediated cleavage of target RNA. Direct contact
Model of Star‐PAP‐mediated cleavage of target RNA. Direct contact

013368718X_CH13_193
013368718X_CH13_193

... 12. The decoding of an mRNA message into a protein ...
Part 4 Transcription
Part 4 Transcription

... Part 4 Transcription ...
Lecture 17 Functional Genetics III Basic Approaches
Lecture 17 Functional Genetics III Basic Approaches

RNA interference was popularized by work in C
RNA interference was popularized by work in C

... substrate for DICER, which cuts it into siRNAs. In turn this can either unwind and prime RNA dependent RNA polymerization, or together with RISC, mediate the cleavage of target mRNAs. This amplification, coupled with RNAi spreading between cells, is thought to underlie germline transmission of RNAi ...
Setting up a transformation--how will the competent cells be treated?
Setting up a transformation--how will the competent cells be treated?

... the cell as synthetic RNAs, replicating viruses or may be transcribed from nuclear genes. – These are recognized and processed into small interfering RNAs by Dicer. – The duplex siRNAs are passed to RISC (RNAinduced silencing complex) – The complex becomes activated by unwinding of the duplex. – Act ...
Chapter 13
Chapter 13

... The duplex may block initiation of translation, cause termination of transcription, or create a target for an endonuclease. ...
Chapter 36: RNA Synthesis and Regulation in Bacteria.
Chapter 36: RNA Synthesis and Regulation in Bacteria.

... Chapter 36: RNA Synthesis and Regulation in Bacteria. 1. Define the three main types of RNA. 2. What are the nucleotides that are used to synthesize RNA? 3. What is the direction of RNA polymerization? 4. What is meant by the statement “RNA polymerization is thermodynamically assisted by PPi hydroly ...
Name
Name

... 2. Proteins are made of smaller molecules called 3. In RNA, adenine pairs with ...
Bio 1 Unit Objectives Protein Synthesis Readings
Bio 1 Unit Objectives Protein Synthesis Readings

... Exploring Life: Chapter 11 (Concepts 11.4, 11.5, 11.6) Exploring Life: Chapter 11 Online Activities ...
Document
Document

... Regulation of gene expression at the level of chromatin Sequence-independent linker histones: control DNA compaction and accessibility to trans-acting factors post-translational modifications of histone tails: control compaction of DNA and serve as docking sites for trans-acting factors Range: Can ...
Isolation of HSV-1 Small RNA Interference Molecules in Latently
Isolation of HSV-1 Small RNA Interference Molecules in Latently

L16 - sRNA Overview
L16 - sRNA Overview

... • RNAi involved in control of viral pathogenesis, and artificial RNAi for study of gene regulation. • Ultimately demonstrated that “natural” RNAi was viable regulatory process, and • The discovery of multiple small regulatory RNAs in eukaryotes, and post transcriptional gene silencing, translational ...
Antisense suppression of replicase gene expression recovers
Antisense suppression of replicase gene expression recovers

... 後轉錄基因靜默化 PTGS(posttranscriptional gene silencing)又名為 RNA 靜默化(RNA silencing),被認為是真核生物 所演化出的種對抗外來基因 Since the only RNA found in a cell should be single stranded, the presence of double stranded RNA signals is an abnormality. The cell has a specific enzyme (in Drosophila it is called Dicer) that recog ...
Presentation title: Introduction to RNA
Presentation title: Introduction to RNA

... The central dogma of genetics is that the genome, comprised of DNA, encodes many thousands of genes that can  be transcribed into RNA. Following this, the RNA may be translated into amino acids  giving a  functional protein.  While the genome of an individual will be identical for each cell througho ...
No Slide Title
No Slide Title

... Guo and Kemphues, Cell 81, 611 (1995) observed that sense and antisense strands worked equally at reducing transcript, – in an anti-sense experiment, a gene is constructed so that it produces a complementary strand to an expressed transcript, • the goal is to complement, thus inactivate the mRNA. ...
messenger RNA (mRNA)
messenger RNA (mRNA)

... ...
Mentor: James A. MacKay Students: Amanda Williams, Holly Sofka
Mentor: James A. MacKay Students: Amanda Williams, Holly Sofka

... Students: Amanda Williams, Holly Sofka Project Description: Ribonucleic acid (RNA) is believed to be an important molecule in the evolution of life and has functionally taken on many important biological roles. Given the many functions of RNA, molecular recognition of RNA represents an attractive go ...
GENE-SILENCING IN THE BRAIN IN VIVO USING siRNA
GENE-SILENCING IN THE BRAIN IN VIVO USING siRNA

< 1 ... 220 221 222 223 224 >

RNA silencing

RNA silencing (associated with the concept of post-transcriptional gene silencing or RNA interference) refers to a family of gene silencing effects by which the expression of one or more genes is downregulated or entirely suppressed by non-coding RNAs, particularly small RNAs. It may also refer to the introduction of a synthetic antisense RNA molecule used in scientific experiments on gene expression. RNA silencing may also be defined as sequence-specific regulation of gene expression triggered by double-stranded RNA (dsRNA). RNA silencing mechanisms are highly conserved in most eukaryotes. The most common and well-studied example is RNA interference (RNAi), in which endogenously expressed microRNA (miRNA) or exogenously derived small interfering RNA (siRNA) induces the degradation of complementary messenger RNA. Other classes of small RNA have been identified, including piwi-interacting RNA (piRNA) and its subspecies repeat associated small interfering RNA (rasiRNA).
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