Phylogenetic Relationships between the Western Aster Yellows
... rRNA gene is a particularly valuable evolutionary marker because it is larger and its rRNA has a more complex secondary structure than the 5s rRNA gene. Because of its central role in cell metabolism, the prokaryote 16s rRNA gene is highly conserved across widely divergent taxa, which frequently exh ...
... rRNA gene is a particularly valuable evolutionary marker because it is larger and its rRNA has a more complex secondary structure than the 5s rRNA gene. Because of its central role in cell metabolism, the prokaryote 16s rRNA gene is highly conserved across widely divergent taxa, which frequently exh ...
using the crispr/cas9 gene editing tool to develop a cure for
... RNA: programmable guide RNA and tracer RNA. These two parts typically function as two separate molecules, but Jennifer Doudna, a researcher at the University of California, Berkeley, was able to combine these two into one complete molecule—one that is now the most commonly used form of CRISPR [6]. T ...
... RNA: programmable guide RNA and tracer RNA. These two parts typically function as two separate molecules, but Jennifer Doudna, a researcher at the University of California, Berkeley, was able to combine these two into one complete molecule—one that is now the most commonly used form of CRISPR [6]. T ...
Gene Section SRSF1 (serine/arginine rich splicing factor 1) -
... modular structure with two RNA recognition motifs (RRM) that provide RNA-binding specificity and one arginine/serine-rich domain (RS), involved in protein-protein interactions that facilitate recruitment of the spliceosome. The RS domain acts also as a nuclear localization signal, controlling the su ...
... modular structure with two RNA recognition motifs (RRM) that provide RNA-binding specificity and one arginine/serine-rich domain (RS), involved in protein-protein interactions that facilitate recruitment of the spliceosome. The RS domain acts also as a nuclear localization signal, controlling the su ...
Expression of Allene Oxide Synthase Determines - Bio
... non-inducing conditions, such as in the absence of wounding or elicitors. Contrary to this, there was a basal level of constitutive expression of the flax AOS under non-inducing conditions in all plant organs (Harms et al., 1998). A similar constitutive level of the AOS protein has also been observe ...
... non-inducing conditions, such as in the absence of wounding or elicitors. Contrary to this, there was a basal level of constitutive expression of the flax AOS under non-inducing conditions in all plant organs (Harms et al., 1998). A similar constitutive level of the AOS protein has also been observe ...
Disclosure All authors have no competing financial relationships to
... TLR7 in PBMCs, resulting in a trend of decreased downstream production of type I IFNs; whereas inhibition of miR-3148 has an opposite effect. ...
... TLR7 in PBMCs, resulting in a trend of decreased downstream production of type I IFNs; whereas inhibition of miR-3148 has an opposite effect. ...
The Mycobacterium tuberculosis katG promoter region contains a
... Mycobacterium tuberculosis katG promoter. A 19 kb region immediately upstream of katG promoted expression of the luciferase gene in E. coli and Mycobacterium smegmatis . A smaller promoter fragment (559 bp) promoted expression with equal efficiency, and was used in all further studies. Two transcri ...
... Mycobacterium tuberculosis katG promoter. A 19 kb region immediately upstream of katG promoted expression of the luciferase gene in E. coli and Mycobacterium smegmatis . A smaller promoter fragment (559 bp) promoted expression with equal efficiency, and was used in all further studies. Two transcri ...
Identification of the mRNA targets of tRNA
... response to rare-tRNA induced ribosomal pausing. Translation is the most resource- and energy-consuming process in the cell, and is therefore highly regulated by a range of protein trans factors in response to environment and nutrient availability (16–22). However, while it is clear that tRNA concen ...
... response to rare-tRNA induced ribosomal pausing. Translation is the most resource- and energy-consuming process in the cell, and is therefore highly regulated by a range of protein trans factors in response to environment and nutrient availability (16–22). However, while it is clear that tRNA concen ...
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... Marcello Forconi, Raghuvir N. Sengupta, Mao-Chin Liu, Alan C. Sartorelli, Joseph A. Piccirilli,* and Daniel Herschlag* The determination of how enzymes achieve their catalytic power requires an understanding of how structural motifs are used to position functional groups of enzymes and substrates wi ...
... Marcello Forconi, Raghuvir N. Sengupta, Mao-Chin Liu, Alan C. Sartorelli, Joseph A. Piccirilli,* and Daniel Herschlag* The determination of how enzymes achieve their catalytic power requires an understanding of how structural motifs are used to position functional groups of enzymes and substrates wi ...
Origin of the catalytic activity of bovine seminal ribonuclease against
... ABSTRACT: Bovine seminal ribonuclease (RNase) binds, melts, and (in the case of RNA) catalyzes the hydrolysis of double-stranded nucleic acid 30-fold better under physiological conditions than its pancreatic homologue, the well-known RNase A. Reported here are site-directed mutagenesis experiments t ...
... ABSTRACT: Bovine seminal ribonuclease (RNase) binds, melts, and (in the case of RNA) catalyzes the hydrolysis of double-stranded nucleic acid 30-fold better under physiological conditions than its pancreatic homologue, the well-known RNase A. Reported here are site-directed mutagenesis experiments t ...
Increased sex chromosome expression and epigenetic
... understood. Mice with deletions of the Y chromosome long arm (MSYq–) have increased spermatid expression of multicopy X and Y genes, and so represent a model for studying post-meiotic sex chromosome repression. Here, we show that the increase in sex chromosome transcription in spermatids from MSYq– ...
... understood. Mice with deletions of the Y chromosome long arm (MSYq–) have increased spermatid expression of multicopy X and Y genes, and so represent a model for studying post-meiotic sex chromosome repression. Here, we show that the increase in sex chromosome transcription in spermatids from MSYq– ...
Regulation of Na/K-ATPase β1 -subunit gene
... beneficial adaptive response to increased workload, but also an independent risk factor for the development of heart failure [17]. Hence, it is important to study the potential hypertrophic effects of drugs that are widely used in the treatment of heart failure. Since the enzyme serves as a receptor ...
... beneficial adaptive response to increased workload, but also an independent risk factor for the development of heart failure [17]. Hence, it is important to study the potential hypertrophic effects of drugs that are widely used in the treatment of heart failure. Since the enzyme serves as a receptor ...
Predicting tRNA and tmRNA genes Aragorn - SEA
... tRNAs to certain regions of the genomes (also called “tRNA clusters” in the phage tRNA literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the others within its genome found by the programs, or encoded on the opposite strand as all ...
... tRNAs to certain regions of the genomes (also called “tRNA clusters” in the phage tRNA literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the others within its genome found by the programs, or encoded on the opposite strand as all ...
asense is a Drosophila neural precursor gene and is
... sense organ precursors (SOPs) and their progeny (Ghysen and O’Kane, 1989). A subset of these cells variably depends on ase (Dambly-Chaudière and Ghysen, 1987; Jarman et al., 1993). Of the first two SOPs to arise in the PNS anlage, the anterior one (the A cell; Ghysen and O’Kane, 1989) gives rise to ...
... sense organ precursors (SOPs) and their progeny (Ghysen and O’Kane, 1989). A subset of these cells variably depends on ase (Dambly-Chaudière and Ghysen, 1987; Jarman et al., 1993). Of the first two SOPs to arise in the PNS anlage, the anterior one (the A cell; Ghysen and O’Kane, 1989) gives rise to ...
pKa of adenine 2451 in the ribosomal peptidyl transferase center
... perturbed pKa, the reported pH-dependent changes in DMS modification of this nucleotide may not be an accurate measure of the protonation status of A2451 base within the ribosome+ In vitro studies of the peptidyl transferase activity of the T. aquaticus large ribosomal subunits carrying mutations at ...
... perturbed pKa, the reported pH-dependent changes in DMS modification of this nucleotide may not be an accurate measure of the protonation status of A2451 base within the ribosome+ In vitro studies of the peptidyl transferase activity of the T. aquaticus large ribosomal subunits carrying mutations at ...
Respiratory terminal oxidases in the facultative chemoheterotrophic
... microns, Sigma-Aldrich) for 4 min. Two-step RT-PCR was performed using the enhanced avian RT-PCR kit (SigmaAldrich). Total RNA was denatured together with the specific primer for 10 min at 85 8C and the reverse transcriptase reaction was performed for 40 min at 48 8C followed by 20 min at 60 8C. PCR ...
... microns, Sigma-Aldrich) for 4 min. Two-step RT-PCR was performed using the enhanced avian RT-PCR kit (SigmaAldrich). Total RNA was denatured together with the specific primer for 10 min at 85 8C and the reverse transcriptase reaction was performed for 40 min at 48 8C followed by 20 min at 60 8C. PCR ...
Knockdown of Parhyale Ultrabithorax - IMBB
... developed a strategy to knock down gene function during Parhayle development using injection of siRNAs, a technique effective in several diverse animal taxa. To demonstrate the efficacy of this technique in Parhyale, we chose the Dll gene as a positive control. Dll and its vertebrate homologs, the D ...
... developed a strategy to knock down gene function during Parhayle development using injection of siRNAs, a technique effective in several diverse animal taxa. To demonstrate the efficacy of this technique in Parhyale, we chose the Dll gene as a positive control. Dll and its vertebrate homologs, the D ...
Multistep process of FUS aggregation in the cell cytoplasm involves
... in detergent-insoluble fraction (Fig. 3D) and formed structures resembling aggregates in cells expressing FUS R522G (Fig. 3B). In contrast, FUS-Npl3 chimeric protein was found only in soluble fraction (Fig. 3D) and its distribution was exclusively diffuse regardless of expression levels (Fig. 3B and ...
... in detergent-insoluble fraction (Fig. 3D) and formed structures resembling aggregates in cells expressing FUS R522G (Fig. 3B). In contrast, FUS-Npl3 chimeric protein was found only in soluble fraction (Fig. 3D) and its distribution was exclusively diffuse regardless of expression levels (Fig. 3B and ...
T-box-mediated control of the anabolic proline biosynthetic genes of
... Bacillus subtilis possesses interlinked routes for the synthesis of proline. The ProJ–ProA–ProH route is responsible for the production of proline as an osmoprotectant, and the ProB–ProA–ProI route provides proline for protein synthesis. We show here that the transcription of the anabolic proBA and ...
... Bacillus subtilis possesses interlinked routes for the synthesis of proline. The ProJ–ProA–ProH route is responsible for the production of proline as an osmoprotectant, and the ProB–ProA–ProI route provides proline for protein synthesis. We show here that the transcription of the anabolic proBA and ...