Inhibition of RNA Synthesis by Anthracycline Analogs
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
Tissue- and Development-specific Expression of Multiple
... nine to L-citrulline. Three isoforms of NOS encoded by distinct genes have been identified. The neuronal and endothelial NO synthases (nNOS, eNOS) are calcium/calmodulin dependent; their expression has in the past been viewed as constitutive (1–4). In contrast, the inducible isoform (iNOS) isolated ...
... nine to L-citrulline. Three isoforms of NOS encoded by distinct genes have been identified. The neuronal and endothelial NO synthases (nNOS, eNOS) are calcium/calmodulin dependent; their expression has in the past been viewed as constitutive (1–4). In contrast, the inducible isoform (iNOS) isolated ...
Differential Regulation of a-Lactalbumin and
... tating Glands.Total mRNAwasisolatedfrom rat mammary control of casein mRNA is suggested by removal of the gland on various days of pregnancy and lactation. Chart 8 pups during lactation; casein messenger levels are dimin shows total protein synthesized as a function of the pg of ished (Chart 9A). Wh ...
... tating Glands.Total mRNAwasisolatedfrom rat mammary control of casein mRNA is suggested by removal of the gland on various days of pregnancy and lactation. Chart 8 pups during lactation; casein messenger levels are dimin shows total protein synthesized as a function of the pg of ished (Chart 9A). Wh ...
Lecture9-Chap24
... • The stop codons UAA (ochre), UAG (amber), and UGA (opal) terminate translation. • In bacteria, they are used most often with relative frequencies UAA>UGA>UAG. ...
... • The stop codons UAA (ochre), UAG (amber), and UGA (opal) terminate translation. • In bacteria, they are used most often with relative frequencies UAA>UGA>UAG. ...
Rabbit Reticulocyte Lysate Technical Manual
... rather than a free amino acid. For more information on the Transcend™ Systems, refer to Technical Bulletin #TB182. An alternative method for non-radioactive translation is the FluoroTect™ GreenLys in vitro Translation System, which allows fluorescent labeling of in vitro translated proteins. More in ...
... rather than a free amino acid. For more information on the Transcend™ Systems, refer to Technical Bulletin #TB182. An alternative method for non-radioactive translation is the FluoroTect™ GreenLys in vitro Translation System, which allows fluorescent labeling of in vitro translated proteins. More in ...
Developing a Gene Therapy for Motor Neuron Disease
... Simplified schematic of SOD1 silencing using short interfering RNAs (siRNA) In untreated cells, SOD1 protein is made as shown on the right along the blue pathway: The SOD1 gene is copied into a messenger RNA which contains the instruction to make SOD1 protein. The SOD1 mRNA is exported to the cytopl ...
... Simplified schematic of SOD1 silencing using short interfering RNAs (siRNA) In untreated cells, SOD1 protein is made as shown on the right along the blue pathway: The SOD1 gene is copied into a messenger RNA which contains the instruction to make SOD1 protein. The SOD1 mRNA is exported to the cytopl ...
A gene expression atlas of a bicoid-depleted
... In developing embryos, gene regulatory networks drive cells towards discrete terminal fates, a process called canalization. We studied the behavior of the anterior-posterior segmentation network in Drosophila melanogaster embryos by depleting a key maternal input, bicoid (bcd), and measuring gene ex ...
... In developing embryos, gene regulatory networks drive cells towards discrete terminal fates, a process called canalization. We studied the behavior of the anterior-posterior segmentation network in Drosophila melanogaster embryos by depleting a key maternal input, bicoid (bcd), and measuring gene ex ...
The Protein Cevalently Linked to the 5'... of Poliovirus RNA by Victor Robert Ambros
... D. Kinetics of removal of VPg from poliovirus RNA in Hela cell extracts........................................ 84 E. Release of VPg from polio RNA in other cell extracts..... 88 F. Glycerol gradient sedimentation of Hela unlinking activity.................................................. 91 G. Mag ...
... D. Kinetics of removal of VPg from poliovirus RNA in Hela cell extracts........................................ 84 E. Release of VPg from polio RNA in other cell extracts..... 88 F. Glycerol gradient sedimentation of Hela unlinking activity.................................................. 91 G. Mag ...
Control of Growth and of the Nuclear Division Cycle in Neurospora
... zoopfii (126), and in the slime mold Physarium polycephalum (124). Many data are also available on the yeast S. cerevisiae. Two groups of workers (19, 185) have shown that RNA per cell (or the RNA/DNA ratio) varies with the growth rate in batch cultures, although in a nonlinear fashion, and less sha ...
... zoopfii (126), and in the slime mold Physarium polycephalum (124). Many data are also available on the yeast S. cerevisiae. Two groups of workers (19, 185) have shown that RNA per cell (or the RNA/DNA ratio) varies with the growth rate in batch cultures, although in a nonlinear fashion, and less sha ...
Constitutive expression of RyhB regulates the heme biosynthesis
... further regulation of this pathway for high ALA production met with many problems because C5 pathway is already highly regulated. For example, HemA and HemL work synergistically, whereas HemA is not stable when heme is present in excess (Wang et al., 1999). Our initial study also indicated that down ...
... further regulation of this pathway for high ALA production met with many problems because C5 pathway is already highly regulated. For example, HemA and HemL work synergistically, whereas HemA is not stable when heme is present in excess (Wang et al., 1999). Our initial study also indicated that down ...
RNA-based regulation of genes of tryptophan synthesis
... capability was dispensed with when the trp aporepressor, while charged and uncharged tRNATrp determine whether transcription organisms evolved that were capable of will or will not be terminated in the operon’s leader region. A poorly expressed internal provides transcripts producing low levels of t ...
... capability was dispensed with when the trp aporepressor, while charged and uncharged tRNATrp determine whether transcription organisms evolved that were capable of will or will not be terminated in the operon’s leader region. A poorly expressed internal provides transcripts producing low levels of t ...
Chapter 14
... 14.15 Lysogeny Requires Several Events • cII and cIII cause repressor synthesis to be established. – They trigger inhibition of late gene transcription. ...
... 14.15 Lysogeny Requires Several Events • cII and cIII cause repressor synthesis to be established. – They trigger inhibition of late gene transcription. ...
Price, DK, Zhang, F, Ashley, CT and Warren, ST: The chicken FMR1 gene is highly conserved containing a CTT 5\' untranslated repeat and encodes an RNA-binding protein. Genomics 31:3-12 (1996).
... cluding material representative of exons 11 and 12 were not present in detectable amounts in chicken brain FMR1 RNA. Indeed, while alternative splicing of exon 12 of the mammalian FMR1 gene has been documented in multiple tissues (Ashley et al., 1993a; Verkerk et al., 1993), alternative splicing eve ...
... cluding material representative of exons 11 and 12 were not present in detectable amounts in chicken brain FMR1 RNA. Indeed, while alternative splicing of exon 12 of the mammalian FMR1 gene has been documented in multiple tissues (Ashley et al., 1993a; Verkerk et al., 1993), alternative splicing eve ...
Kallikrein-like prorenin-converting enzymes in inbred
... Tissue preparation. Part of the surgically removed submandibular gland was frozen in liquid nitrogen for further use, whereas the rest of it was fixed in chilled phosphate buffered saline (PBS) containing 3% paraformaldehyde and 0.02% diethylpyrocarbonate for 2–3 h. Preparation of tissue extract. One ...
... Tissue preparation. Part of the surgically removed submandibular gland was frozen in liquid nitrogen for further use, whereas the rest of it was fixed in chilled phosphate buffered saline (PBS) containing 3% paraformaldehyde and 0.02% diethylpyrocarbonate for 2–3 h. Preparation of tissue extract. One ...
Skeletal muscle actin mRNA. Characterization of the 3
... inserts that were sequenced are indicated by dotted lines. Restriction enzymes that were used and the position of their cleavage sites are indicated. The alignment of the cDNA inserts of plasmids p749 and pl50 with actin mRNA was made by comparing the amino acid sequences deduced from the nucleotide ...
... inserts that were sequenced are indicated by dotted lines. Restriction enzymes that were used and the position of their cleavage sites are indicated. The alignment of the cDNA inserts of plasmids p749 and pl50 with actin mRNA was made by comparing the amino acid sequences deduced from the nucleotide ...
Molecular Characterization of NADH-Dependent
... used as a hybridization probe to screen a third cDNA library that had been prepared using a reverse transcriptase lacking RNase H activity. This screening produced numerous hybridizing recombinant phage that contained inserts longer than those previously obtained, including one which was of a size t ...
... used as a hybridization probe to screen a third cDNA library that had been prepared using a reverse transcriptase lacking RNase H activity. This screening produced numerous hybridizing recombinant phage that contained inserts longer than those previously obtained, including one which was of a size t ...
Creating conditional dual fluorescence labelled transgenic animals
... (miRNA) and long non-coding RNA (lncRNA) have been shown to be involved in gene regulation at post-transcriptional and translational levels. For example, microRNAs, about 22 nucleotide long, are among the largest known classes of non-coding RNAs involved in gene silencing with more than 1,000 predic ...
... (miRNA) and long non-coding RNA (lncRNA) have been shown to be involved in gene regulation at post-transcriptional and translational levels. For example, microRNAs, about 22 nucleotide long, are among the largest known classes of non-coding RNAs involved in gene silencing with more than 1,000 predic ...
Coordination of replication and transcription along a Drosophila
... Stillman 2003). As eukaryotic cells enter S phase, a subset of these assemblies are directed to initiate replication in a defined temporal order (Fangman and Brewer 1992). Although the large majority of replication initiation factors are conserved in all eukaryotes, how metazoan origins of replicati ...
... Stillman 2003). As eukaryotic cells enter S phase, a subset of these assemblies are directed to initiate replication in a defined temporal order (Fangman and Brewer 1992). Although the large majority of replication initiation factors are conserved in all eukaryotes, how metazoan origins of replicati ...
EIN4 and ERS2 Are Members of the Putative Ethylene Receptor
... The emerging picture is that ethylene signal transduction is performed by a phosphorylation cascade that might modulate the activity of transcription factors regulating gene expression. The ethylene signal appears to be perceived by a family of ethylene receptors. Three members of the putative ethyl ...
... The emerging picture is that ethylene signal transduction is performed by a phosphorylation cascade that might modulate the activity of transcription factors regulating gene expression. The ethylene signal appears to be perceived by a family of ethylene receptors. Three members of the putative ethyl ...