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... alleles of the PEGs. However, a deletion on the paternal chromosome does not have this effect (Lin et al., 2003). The mechanism by which the IG-DMR regulates Dlk1-Gtl2 imprinting is unclear. It is likely that the IG-DMR acts as a positive regulator activating Gtl2 transcription and its downstream no ...
... alleles of the PEGs. However, a deletion on the paternal chromosome does not have this effect (Lin et al., 2003). The mechanism by which the IG-DMR regulates Dlk1-Gtl2 imprinting is unclear. It is likely that the IG-DMR acts as a positive regulator activating Gtl2 transcription and its downstream no ...
Widespread expression of the bovine Agouti gene results from at
... promoter sequences because of the repetitive content of its 5¢UTR. However, this C promoter sequence that remains to be isolated must be localized between exon 3A and 2 or upstream A promoter. The two 5¢UTR sequences corresponding to the 2 and 1.5 kb lung mRNA transcripts are reported here. The A an ...
... promoter sequences because of the repetitive content of its 5¢UTR. However, this C promoter sequence that remains to be isolated must be localized between exon 3A and 2 or upstream A promoter. The two 5¢UTR sequences corresponding to the 2 and 1.5 kb lung mRNA transcripts are reported here. The A an ...
Identification of novel endogenous antisense transcripts by DNA
... conserved NATs (antisense of Acaa1b and Aard) by performing Northern and in situ hybridization (ISH) analyses. Whereas human ACAA1 (acetyl-Coenzyme A acyltransferase 1) overlaps with DLEC1 (deleted in lung and esophageal cancer 1) in a tail-to-tail overlapping manner, its orthologous counterpart in ...
... conserved NATs (antisense of Acaa1b and Aard) by performing Northern and in situ hybridization (ISH) analyses. Whereas human ACAA1 (acetyl-Coenzyme A acyltransferase 1) overlaps with DLEC1 (deleted in lung and esophageal cancer 1) in a tail-to-tail overlapping manner, its orthologous counterpart in ...
Inhibition of the Particle-associated RNA
... various compounds were tested in parallel in the same experiment using a single virus preparation. Unless otherwise stated, inhibitory compounds at a range of different concentrations from o-I to o.ooi mM were added last to the RNA polymerase reaction mixture at 4 °C, immediately before warming to 3 ...
... various compounds were tested in parallel in the same experiment using a single virus preparation. Unless otherwise stated, inhibitory compounds at a range of different concentrations from o-I to o.ooi mM were added last to the RNA polymerase reaction mixture at 4 °C, immediately before warming to 3 ...
Type XVII collagen gene mutations in junctional epidermolysis
... In recent years data have accumulated that indicate that the spliceosome can be a target for attempting gene correction. The spliceosome of a cell is the site where small-nuclear ribonucleoproteins facilitate removal of introns from premtRNA (the so-called splicing reaction) and therefore control th ...
... In recent years data have accumulated that indicate that the spliceosome can be a target for attempting gene correction. The spliceosome of a cell is the site where small-nuclear ribonucleoproteins facilitate removal of introns from premtRNA (the so-called splicing reaction) and therefore control th ...
Serine/Arginine-rich proteins Physcomitrella patens Andreas Ring
... containing PTCs are often not translated; they are rather the target of nonsense-mediated decay (NMD) which is a RNA surveillance system that prevents the synthesis of truncated and potentially hazardous protein products (Belgrader et al., 1994; Maquat, 2004). A recent survey of alternatively splice ...
... containing PTCs are often not translated; they are rather the target of nonsense-mediated decay (NMD) which is a RNA surveillance system that prevents the synthesis of truncated and potentially hazardous protein products (Belgrader et al., 1994; Maquat, 2004). A recent survey of alternatively splice ...
Two Genes with Similarity to Bacterial Response Regulators Are
... cytokinins and alterations of endogenous cytokinin levels using transgenes and by the correlation of endogenous levels with various developmental processes (Binns and Thomashow, 1988; Mok and Mok, 1994). Very little is known about the mechanisms underlying cytokinin perception and signaling. One lik ...
... cytokinins and alterations of endogenous cytokinin levels using transgenes and by the correlation of endogenous levels with various developmental processes (Binns and Thomashow, 1988; Mok and Mok, 1994). Very little is known about the mechanisms underlying cytokinin perception and signaling. One lik ...
Figures and figure supplements
... Figure 8—figure supplement 1. Cartoons of lineages that showed two RFP-to-GFP switches in directly related cells. Switches are labeled with the numbers 1 and 2. In pedigree A, onset of GFP expression (depicted as yellow) was detected in a large-budded cell (1). At the same time, GFP also appeared in ...
... Figure 8—figure supplement 1. Cartoons of lineages that showed two RFP-to-GFP switches in directly related cells. Switches are labeled with the numbers 1 and 2. In pedigree A, onset of GFP expression (depicted as yellow) was detected in a large-budded cell (1). At the same time, GFP also appeared in ...
Horizontal, agarose systems
... The gel cassette is designed to open with Invitrogen’s Gel Knife, so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis. Nucleic acid markers The room-temperature stable, ready-to-use E-Gel® 1Kb Plus DNA Ladder is premix ...
... The gel cassette is designed to open with Invitrogen’s Gel Knife, so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis. Nucleic acid markers The room-temperature stable, ready-to-use E-Gel® 1Kb Plus DNA Ladder is premix ...
Regulatory Genes Controlling MPG7 Expression
... regulatory genes render strains unable to utilize a wide variety of nitrogen sources, with the exception of ammonium or glutamine. Our study of MPG7 led us to hypothesize that mutations in a wide-domain regulator of NR might render M. grisea nonpathogenic due to an inability to derepress a subset of ...
... regulatory genes render strains unable to utilize a wide variety of nitrogen sources, with the exception of ammonium or glutamine. Our study of MPG7 led us to hypothesize that mutations in a wide-domain regulator of NR might render M. grisea nonpathogenic due to an inability to derepress a subset of ...
A Primer on Gene Regulation
... the electrophoretic mobility shift assay (Figure 8). A segment of DNA containing an operator site is subjected to electrophoresis through a gel in the presence of an electric field in which the negative pole is at one end (top end in the figure) of the gel and the positive pole at the other. The DNA ...
... the electrophoretic mobility shift assay (Figure 8). A segment of DNA containing an operator site is subjected to electrophoresis through a gel in the presence of an electric field in which the negative pole is at one end (top end in the figure) of the gel and the positive pole at the other. The DNA ...
Research Resources: Comparative MMM icroRNA
... During folliculogenesis, cumulus cells surrounding the oocyte differentiate into corona radiata cells (CRCs) and cumulus oophorus cells (COCs), which are involved in gonadal steroidogenesis and the development of germ cells. Several studies suggested that microRNAs (miRNAs) play an important regulat ...
... During folliculogenesis, cumulus cells surrounding the oocyte differentiate into corona radiata cells (CRCs) and cumulus oophorus cells (COCs), which are involved in gonadal steroidogenesis and the development of germ cells. Several studies suggested that microRNAs (miRNAs) play an important regulat ...
The role of Dby mRNA in early development of male mouse zygotes
... oocyte in this process is well understood. The oocyte contributes most of the molecular components, including cytosol, organelles and large stores of maternal mRNA required for embryogenesis. In contrast, the paternal contribution of sperm is rarely considered because the mammalian ejaculated sperma ...
... oocyte in this process is well understood. The oocyte contributes most of the molecular components, including cytosol, organelles and large stores of maternal mRNA required for embryogenesis. In contrast, the paternal contribution of sperm is rarely considered because the mammalian ejaculated sperma ...
Document
... rescued by inserting a C. This rate is in general smaller than the base deletion rate. For codons with a vacant site, they also undergo insertions which randomly insert one of the four nucleotides (A,C,G,U) back into the vacant site with a mutation rate µe , which we take to be the same as the rando ...
... rescued by inserting a C. This rate is in general smaller than the base deletion rate. For codons with a vacant site, they also undergo insertions which randomly insert one of the four nucleotides (A,C,G,U) back into the vacant site with a mutation rate µe , which we take to be the same as the rando ...
Bacterial disease resistance of transgenic hybrid poplar expressing
... subjected to polymerase chain reaction (PCR), reverse transcription-polymerase chain reaction (RT-PCR) and RNA dotblot analyses (see next section). Transgenic plants, when confirmed, were propagated in vitro from stem cuttings. Plantlets were then transplanted to soil medium PromixTM (Premier Tech, ...
... subjected to polymerase chain reaction (PCR), reverse transcription-polymerase chain reaction (RT-PCR) and RNA dotblot analyses (see next section). Transgenic plants, when confirmed, were propagated in vitro from stem cuttings. Plantlets were then transplanted to soil medium PromixTM (Premier Tech, ...
Biochemistry Lecture 23 THE LAST ONE!
... Polysomes • >1 Ribosome at a time translates a single mRNA (27-29) – Efficient use of single mRNA strand ...
... Polysomes • >1 Ribosome at a time translates a single mRNA (27-29) – Efficient use of single mRNA strand ...
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... intron’s catalytic activity (Davies et al., 1982; Michel et al., 1982). Figure 2a shows one of the two originally proposed secondary structures from phylogenetic covariation (Michel and Dujon, 1983). Below we describe how these structures were further developed into models that include secondary and ...
... intron’s catalytic activity (Davies et al., 1982; Michel et al., 1982). Figure 2a shows one of the two originally proposed secondary structures from phylogenetic covariation (Michel and Dujon, 1983). Below we describe how these structures were further developed into models that include secondary and ...
Chapter 11 Lecture PowerPoint - McGraw Hill Higher Education
... denote transcription of class II genes) • 13 TAFs have been identified and associated with class II preinitiation complexes • The core TAFs were first named according to their molecular mass but have now been renamed according to their sizes, from largest to smallest • Several functions discovered: ...
... denote transcription of class II genes) • 13 TAFs have been identified and associated with class II preinitiation complexes • The core TAFs were first named according to their molecular mass but have now been renamed according to their sizes, from largest to smallest • Several functions discovered: ...
Descriptions of Banbury Conference
... binding protein and upon the likelihood that the phenotype of FXS reflects where and if the mRNAs that FMRP are translated within the neuron (or other cell). Of particular interest were the first reports, independently discovered by Herve Moine and Jennifer Darnell, of a motif in mRNA to which FMRP ...
... binding protein and upon the likelihood that the phenotype of FXS reflects where and if the mRNAs that FMRP are translated within the neuron (or other cell). Of particular interest were the first reports, independently discovered by Herve Moine and Jennifer Darnell, of a motif in mRNA to which FMRP ...